Abstract: Objective To investigate the relationship among the expressions of heme-oxygenase-1 (HO-1), glial fibrillary acidic protein (GFAP) and cyclinD1 in the perihematomal tissues in human intracerebral hemorrhage (ICH) and brain renovation following ICH. Methods Paraffin?embedded brain tissues of 32 human fatal cases of ICH from the perihematomal tissues. The tissues from the hemorrhagic lesion side, as well as tissues from the corresponding area at the opposite side as controls, were stained with HE and immunohistochemistry staining. The expressions of HO-1, GFAP and cyclinD1 in the perihematomal tissues were analysed with SPSS 11.5 software. Results Expression of HO-1 could be detected in the specimens of the 2 h group [(5.30 ± 1.00)/HP]. The GFAP and cyclinD1 positive astrocytes in the perihematomal tissues were also identified at 2 h group [(22.60 ± 1.40)/HP, (11.50 ± 1.20)/HP, respectively]. All of their levels decreased gradually after the peak. The positive astrocytes of hemorrhagic lesion side at different time points were higher than contralateral expression in normal brain tissues. The difference was statistically significant. Conclusion Expressions of HO-1, GFAP and cyclinD1 in the perihematomal tissues were "parabola". The increased expressions of HO-1 and cyclinD1 after ICH provide a potential protection or destruction mechanism after ICH in human.

Key words: Cerebral hemorrhage, Astrocytes, Heme oxygenase (decyclizing), Glial fibrillary acidic protein, Cyclins, Immunohistochemistry

摘要： 目的 探讨脑出血后血肿周围组织血红素氧合酶-1（HO-1）、胶质纤维酸性蛋白（GFAP）和细胞周期蛋白D1（cyclinD1）表达规律，及其与神经修复之间的关系。方法 HE染色观察脑出血后神经元和星形胶质细胞形态变化，免疫组织化学染色检测脑出血后不同时间点血肿周围组织HO-1、GFAP 和cyclinD1 表达水平。结果 脑出血后2 h 星形胶质细胞胞质内即开始表达HO-1［（5.30 ± 1.00）个/高倍视野］、GFAP［（22.60 ± 1.40）个/高倍视野］和cyclinD1［（11.50 ± 1.20）个/高倍视野］，达峰值水平后逐渐下降，脑出血后不同时间点表达水平均高于健侧正常脑组织，且差异具有统计学意义（均P = 0.000）。结论 人脑出血后血肿周围组织HO-1、GFAP 和cyclinD1 表达变化呈“抛物线”样，HO-1 和cyclinD1 共同参与了脑出血后星形胶质细胞的增生与活化，以及脑出血后的继发性损伤和修复。

关键词: 脑出血, 星形细胞, 血红素氧化酶（脱环）, 神经胶质原纤维酸性蛋白质, 细胞周期蛋白类, 免疫组织化学