Table of Content

05 November 2014, Volume 34 Issue 11

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Up-expression of cold-sensitive receptor and its clinical significance in subjects with chronic obstructive pulmonary disease

2014, 34(11):  1453-1457. 

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Objective To explore the different expression of transient receptor potential protein -8 (TRPM8) and related signal transduction mechanism in airway epithelial tissue between the patients with COPD and healthy subjects. Methods The distribution and different levels of TRPM8 protein in human bronchial epithelial was viewed by immunohistochemistry. TRPM8 protein and mRNA were further detected by PCR and Western blot, respectively. The 16HBE human airway epithelial cells were stimulated with cold temperature (18℃). Cells were divided into 5 groups：a control group (incubated at 37℃)，a cold stimulation group, a cold stimulation +BCTC group, a cold stimulation+TRPM8 shRNA group, a cold stimulation+control shRNA group. The relative concentration of intracellular Ca2+ were measured by laser confocal. Results TRPM8 protein was mainly distributed in the membranes of basal cells or small particle cells within the bronchial epithelium. The ratio of integrated optical to area and the levels of TRPM8 mRNA and protein within the bronchial epithelium were significant higher in COPD group when compared with control group（P<0.05）.The relative concentration of intracellular calcium in cold stimulation group was higher than that of control group(P<0.05). BCTC and TRPM8 shRNAreduced intracellular calcium, compared with single cold stimulation group (P<0.05). Conclusion It is possible that up-expression of TRPM8 in subjects with COPD will be coupled with a greater sensitivity to cold than normal subjects.

Effect of retinoic acid in inducement of nuclear transfer embryonic stem cells to differentiate into male germ cells

2014, 34(11):  1458-1462. 

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Objective To set up a feasible method of differentiation of male germ cells from mouse nuclear transfer embryonic stem cell in vitro. Methods We constructed mouse reconstructed embryos. The NT-ESCs like colonies were identified. The NT-ESCs were digested and divided into 4 groups : group ⑴ were induced to form embryoid bodies and cultured in 2μmol/L retinoic acid( RA) inducing them to proliferate and differentiate into male germ cells; Then passage 1 NT-ESCs as group ⑵; Group ⑶ underwent EB culture; Group ⑷ underwent EB culture and were primary cultured. And the mouse embryonic fibroblast cell as group ⑸. Differences in mRNA expression (Oct-4、VASA、Scp3、Sry、Tex14 and β-actin) were detected by RT-PCR. The proteins of VASA and Scp3 were analyzed by immunofluorescence assay. Results Most of NT-ESCs had typical characteristics of mouse embryonic stem cells . The genes related to male germ cells development were expressed in the NT-EBs cells, and the mRNA expression of Oct-4、VASA、Scp3、Sry、Tex14 and β-actin in the induced NT-EBs were positive. Those in control groups were negative. The fluorescence intensity of Scp3 in the induced NT-EBs was high(+);The fluorescence intensity of VASA in the induced NT-EBs was higher(+) than that in the control group(±). Conclusion NT-ESCs could be differentiated into male germ cells in vitro under induction of retinoic acid.

Preparation of the ovarian cancer targeted MR molecular probe

2014, 34(11):  1463-1467. 

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Objective To prepare an ovarian cancer targeted MR molecular probe, and evaluate the feasibility on SKOV3 cells transfection. Methods The optimal weight proportion of superparamagnetic iron oxide modified with Poly-L-lysine（SPIO-PLL） and plasmid pGenesil1-shRNA in probe was determined through agarose gel retardation experiments. Cells transfection with probes was evaluated with fluorescence microscope for green flourescent protein expression detecting. The intracellular distribution of iron particals contained in probes was observed by transmission electron microscope .The cellular toxicity of probes was explored with CCK-8 method. The changes of cells in MR T2* signal intensities were detected after transfection. Result Agarose gel retardation experiments resulted that SPIO-PLL could effectively bind plasmid when the weight ratio of SPIO-PLL and plasmid reached 6:1. After cells transfection with the probes, green fluorescene could be observed, and iron particals could be detected in the cytoplasm. The cell survival rate was higher than 80% when the concentration was less than 50 mg/L. There were significant T2* signal intensities reduction after cells transfected with probes（P<0.01）. Conclusion An ovarian cancer targeted MR molecular probe can be successfully prepared, which can succeed in transfecting ovarian cancer SKOV3 cells with low cellular toxicity, and be detected by MR scanning sensitively.

Acteoside attenuate MPP+-induced PC12 cell injury via induction of heme oxygenase-1 expression

2014, 34(11):  1468-1471. 

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Objective To investigate whether acteoside (AS) protect PC12 cells against MPP+ -induced neurotoxicity through induction of HO-1 expression. Methods PC12 cells were stimulated with AS(1、20 and 30 μmol/L) for 12 h, RT-PCR was used to analyze HO-1 mRNA expression, and western blot and confocal microscopic analysis was applied to determine the expression of HO-1 protein. Cells were pre-incubated with vehicle or AS for 2h, followed by incubation with 1 mmol/L MPP+ for another 24 h，or cells were pretreated with Zinc Protoporphyrin (ZnPP) for 1 h in the absence or presence of AS and were exposed to MPP+ for 24 h，cell viability was measured by MTT assay. Results AS significantly reduced MPP+-induced the loss of cell viability. AS induced the expression of HO-1 in PC12 cells at mRNA and protein level. Acteoside-induced HO-1 which was predominantly localized to the cytoplasm. The HO-1 inhibitor ZnPP markedly abolished the neuroprotective effect of AS against MPP+-induced neurotoxicity. Conclusions Acteoside induce HO-1 expression, thereby attenuating MPP+-induced PC12 cell injury.

The analysis of serum lactoferrin levels in patients with systemic lupus erythematosus

2014, 34(11):  1472-1476. 

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Objective To compare serum levels of lactoferrin (LF) in healthy subjects and patients with systemic lupus erythematosus (SLE). To analyze the cause for reduced LF levels in SLE patients. Methods Serum concentrations of lactoferrin in healthy children (n = 38), healthy adults (n = 46), pediatric SLE patients (n = 38), adult SLE patients (n = 46), healthy people with ages from 1 to 80 (n = 80), IgA nephropathy patients (n = 29) and other kidney patients (n = 19) were measured by ELISA. Total protein concentrations in serum were measured by BCA. An unpaired t test was performed to analyze the differences between two groups and Spearman's correlation analysis was performed to analyze the relationship between the levels of LF and total protein. Results Serum concentrations of LF in both pediatric and adult patients were lower than that in healthy control subjects, patients with IgA nephropathy and other kidney patients (P<0.001). The levels of LF were reduced in people aged over 50. Conclusion LF levels were significantly reduced in SLE patients, which was not caused by the changes of total protein in serum.

Ano2 is the molecular identity of calcium-activated chloride channel

2014, 34(11):  1477-1481. 

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Objective To investigate that Ano2 is the molecular identity of calcium-activated chloride channels. Methods The full length coding sequence of Ano2 was amplified by RT-PCR，Ano2 and eukaryotic expression vector pEGFP-N3 were ligated；Then recombinant plasmids were transfected into FRT cells using liposome，and the stable transfection FRT cells were selected with antibiotic; the expression and location of Ano2 was observed by the inverted fluorescence microscope and its expression was detected by western blot；the electrophysiological properties of Ano2 were researched by whole-cell patch-clamp technique. Results pEGFP-Ano2 was constructed successfully，FRT cell membrane expressed Ano2；The electrophysiological properties of Ano2 was Ca2+-、time- and voltage-dependent，and an outward-rectifying I/V relationship was observed. Conclusion Ano2 is the molecular identity of calcium-activated chloride channels.

Effects of rHSG on small airway resistance of COPD rats and the expression of TGF-β1 in fibroblasts

2014, 34(11):  1482-1485. 

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Objective By testing the normal rat, rat COPD model the expression of small airway fibroblasts rHSG, to look for the correlation of small airway resistance、TGF-β1 and rHSG , to explore rHSG regulation mechanism of airway remodeling. Methods Rats were randomly divided into normal group and module, to test lung function and TGF - beta 1 contenting in alveolar lavage fluid respectively. Training by applying the method of tissue mass attached airway fibroblasts, using RT-PCR and Western blot detection rHSGmRNA and protein expression. To analyze rHSG gene quantity and small airway resistance and TGF - beta 1 correlation. Results Build module rats have a cough, wheeze, stRidor and phlegm syndrome accompanied by depression, diet reduce, hair yellow, after weight loss, lung volume increases slightly, lung compliance decreased obviously, and airway resistance was significantly higher than the normal group( P < 0.01); TGF-β1 contented in alveolar lavage fluid was significantly higher than that of normal group (P < 0.01); rHSG genes expressed in normal group is higher than building group (P < 0.05), rHSGmRNA and protein are negatively correlated with RI, TGF-β1, and were positively correlated with Cdyn. Conclusions rHSG may through regulate the TGF-β1 level to intervent airway remodeling process.

Inhibition of selective mitochondrial fission inhibitor on apoptosis after acute spinal cord injury in rats

2014, 34(11):  1486-1490. 

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Objective To detect the effects of the pretreatment of Mdivi-1on mitochondrial membrane potential, the release of AIF, and apoptosis after acute spinal cord injury (ASCI) in rats. Methods Rats were randomly divided into sham operation group (Sham group), single spinal cord injury group (SCI group) , DMSO pretreatment group（DMSO group），Mdivi-1 pretreatment group (Mdivi-1 group). Allen's weight drop method was used to establish ASCI rat model at T10 section. The content of AIF protein in the mitochondria and nucleus in spinal cord of rats was detected by western blot. Mitochondrial membrane potential was deteced by JC-1 labeling. The number of apoptotic cells were assessed by TUNEL staining. Results Compared with Sham group , AIF in mitochondria of SCI group reduced first, then increased ,and the lowest point is 8h(P<0.01); while AIF in the nucleus showed the opposite trend(P<0.01); The mitochondrial membrane potential decreased significantly, while the number of apoptotic cells increased significantly at 8h after ASCI(P<0.01). Compared with SCI group,In Mdivi-1 group, The mitochondrial membrane potential, AIF in mitochondria increased significantly (P<0.01), however, AIF in nucleus (P<0.01) and the number of apoptotic cells reduced significantly at 8h after ASCI (P<0.01). Conclusion Mdivi-1 can protect mitochondrial membrane potential, inhibit the release of AIF in mitochondria and the apoptosis after acute spinal cord injury.

Effects of β-catenin on cell migration、invasion and apoptosis of TE85 osteosarcoma cells

2014, 34(11):  1491-1496. 

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Objective To investigate the effect of β-catenin on the migration, invasion and apoptosis of osteosarcoma cell line TE85. Methods The mRNA and protein expression of total β-catenin in different osteosarcoma cell lines were detected by RT-PCR and Western blot, and the osteosarcoma cell line TE85 was infected with Adβ-catenin, then RT-PCR and the reporter TOP-Luc were used to determine the expression of β-catenin and the the β-catenin/TCF4 activity of TE85 cells.The cell migration and invasion were detected by scratch wound assay, and Transwell, respectively. DAPI staining and FCM were performed to detect cell apoptosis. Results The endogenous expression of β-catenin of TE85 cells was relatively lower in osteosarcoma cell line compared with other osteosarcoma cells. The mRNA expression level of β-catenin was increased after infection, as well as the activity of β-catenin/TCF4. Scratch test and Transwell results showed that the migration and invasion capacity of cells significantly decreased after Adβ-catenin infection (P<0.05). However, the overexpression of β-catenin resulted in no significant changes in cell apoptosis. Conclusion β-catenin can effectively inhibit the migration, invasion ability of the TE85 cells, but does not affect on its apoptosis. Our findings suggest that β-catenin may be providing a new target for the treatment of osteosarcoma.

Correlation of NF-κB and malignant transformation of Rat BMSCs in C6 glioma microenvironment

2014, 34(11):  1497-1502. 

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Objective To investigate whether the activation and over expression of NF-κB and STAT3 play an important role in the malignant transformation of BMSCs in C6 glioma microenvironment. Methods To simulate the abnormal microenvironment，the conditioned medium of C6 glioma cells was used to culture BMSCs to build a co-cultured system .Cell surface markers were assessed by flow cytometry .The cells’ morphological features were observed by phase contrast Microscope. The IL-6 levels of cells’ supernatant were measured by ELISA assay. The protein and mRNA expression of STAT3 were tested by Real-time PCR and western blot. The mRNA expressions of NF-κBp65 and C-MYC were also measured by Real-time PCR. Results BMSCs were negative for CD45, but positive for CD29, CD90 .The experimental group cells showed typical morphology features of tumor cell. The IL-6 level in the experimental group(16.7±2.9ng/L)was significantly higher than that in the negative group（12.62±3.20 ng/L）.NF-κBp65,P-STAT3,C-MYC protein were significantly higher than the negative control group and blank group(P<0.05),and they were mainly situated in the nuclei, presenting in an activation state. Conclusion The malignant transformation of BMSCs was significantly related to the over expression and activation of NF-κBp65,STAT3.

Unsaturated fatty acids improves the lipid composition of myocardium and the mitochondrial function of myocardium after ischemia/reperfusion in mice

2014, 34(11):  1503-1507. 

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Objective To investigate the influence of increasing the proportion of plant oil in the diet on the ω-6 /ω-3 PUFAs ratio of myocardium and the effect of those on the infection status and mitochondrial function in the at risk area after ischemia /reperfusion. Method 80 C57BL/6 mice, randomly divided into ordinary diet group and plant oil group(n=40/group). One month later, myocardial ischemia/reperfusion model was built, mitochondria were isolated by differential centrifugation. Oxygen consumption was determined by oxygen electrode. 2,3,5 triphenyltetrazolium chloride (TTC) staining was used to measure infarction size. real time reverse transcription-polymerase chain reaction(RT-PCR) was used to detect the mRNA expression of TNF-a, amount of TNF-a and IL-1 were measured by ELISA. Results: compared with the ordinary diet group, in the plant oil group, ω-6 /ω-3 PUFAs ratio of myocardium was lower , after ischemia/reperfusion, the mRNA expression of TNF-a, amount of TNF-a and IL-1 in the area at risk was lower , the respiration control rate of myocardium in the area at risk improved apparently and the Infarction size were diminished. Conclusion: unsaturated fatty acids improves the lipid composition of myocardium , improved the mitochondrial function after ischemia/reperfusion in mice.

SULT2B1 interference inhibits migration and invasion ability of mouse hepatocellular carcinoma Hepa1-6 cells in vitro by down-regulating MMP-2 expression

2014, 34(11):  1508-1513. 

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Objective To investigate the effect and molecular mechanism of hydroxysteroid sulfotransferase 2B1 (SULT2B1) on the migration and invasion ability of mouse hepatocellular carcinoma Hepa1-6 cells in vitro. Methods Hepa1-6 cells were divided into SULT2B1 interference group and conrol group, which infected with lentivirus-mediated SULT2B1 vector and GFP-LV vector respectively. The invasion and migration ability of treated Hepa1-6 cells were examined by Transwell assay. The mRNA levels of matrix metalloproteinase-2 (MMP-2), MMP-9 and tissue inhibitor of metalloproteinase-2 (TIMP-2) were determined by Real-time PCR analysis. MMP-2 and MMP-9 activities in tumor conditioned medium (TCM) of Hepa1-6 cells were detected by gelatin zymography. Results The number of Hepa1-6 cells with SULT2B1 interference invaded or migrated through the membrane without and with matrigel decreased significantly as compared with the control group (P< 0.05). MMP-2 mRNA level was down regulated in Hepa1-6 cells with SULT2B1 interference, while TIMP-2 was up-regulated in the opposite way (P< 0.05). The MMP-2 activity in TCM of Hepa1-6 cells with SULT2B1 interference was decreased obviously (P<0.05). Conclusion Knock-down of SULT2B1 can inhibit the migration and invasion ability of Hepa1-6 cells, which role is mainly associated with down-regulating MMP-2 and up-regulating TIMP-2 levels.

Effects of hsp70 on the glucose metabolism in C2C12 cells

2014, 34(11):  1514-1518. 

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Objective To examine the effects of over-expression Hsp70 on the glucose metabolism in C2C12 cells. Methods C2C12 cells were divided into two groups: transfected group and normal control. Hsp70 gene was amplified from pAT153 plasmids and then cloned into pTRE2hyg vector. After the transfection of recombinant plasmids of pTRE2hyg- Hsp70 into the C2C12 cells, the expression of Hsp70 was examined by Western blot and the C2C12 cell line of up-regulated Hsp70 was established. Glucose consumption and glucose uptake were measured by the glucose oxidase method and 3H-labeled glucose respectively at 3 and 7 days after cellular differentiation in two groups. Changes in lactate production were determined by biochemical analyzer. In addition, activities of enzymes involved in glycolysis (phosphofructokinase, PFK; lactate dehydrogenase, LDH; hexokinase, HK; pyruvate kinase, PK) and the intracellular ATP level were evaluated by colorimetry in C2C12 cells. The expression of plasma membrane Glut4 in protein level was measured by Western-blot. Results Compared with controls, the glucose consumption, glucose uptake and lactate excretion were significantly elevated in cells expressing up-regulated Hsp70 (p<0.01) at 3 and 7 days after cellular differentiation. The activities of enzymes involved in glycolysis ( PFK, LDH, HK, PK ) and the intracellular ATP level were significantly increased (p<0.01) in the transfected C2C12 cells. In addition, the expression of plasma membrane Glut4 in protein level was increased in transfected C2C12 cells (p<0.05) . Conclusion Hsp70 can promote glucose uptake by increasing the expression of plasma membrane Glut4 in C2C12 cells. And Hsp70 can also increase the glycolytic capacity by increasing the activities of glycolytic enzymes in C2C12 cells.

Effects of treadmill exercise on the rapidly activating IKr channel of rat sinoatrial node

Bing Bo

2014, 34(11):  1519-1523. 

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Objective This paper discusses the effects of two weeks exhaustive treadmill running on rapidly activating IKr channel of rat sinoatrial node. Methods SD rats were equally assigned into 5 groups，including 1 control groups（C group）, 2 groups undergoing single exhaustive treadmill running（O group）, and 2 groups undergoing two weeks repeated treadmill running（R group）,10 rats each group. After two weeks normal feeding，single exhaustive treadmill running was carried out for the O group. Exhaustive treadmill running was carried out for the R groups 6 days per week for total of two weeks. The samples were drawn from rats at 0 and 24 hours after exercise. The rats of O group were named O-0h、O-24h, respectively, the rats of R group were named R-0h、R-24h, respectively. The mRNA and protein expression of IKr channel subunit in sinoatrial node were analyzed by real-time fluorescent quantitative PCR and Western Blot，the current density of IKr channel were analyzed by whole-cell patch clamp analysis. Results 1) The ERG1b mRNA and protein expression of the repeated treadmill running R-0h and R-24h group were significantly lower than that of C and O-0h group（P<0.01）. 2) The current density of IKr of the repeated treadmill running R-0h and R-24h group were 0.89±0.03pA/pF、1.02±0.01pA/pF respectively, significantly lower than that of C and O-0h group（P<0.01, P<0.01）. Conclusion Exhaustive exercise downregulated the sinoatrial node ERG1b gene subunit expression, along with the corresponding current IKr, and may be one of the most important mechanism of exercise-induced sinoatrial node dysfunction and arrhythmia.

Let-7a targeted inhibition of Ewing's sarcoma CDK6 expression

2014, 34(11):  1524-1529. 

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Objective We amied to identify the direct target gene of microRNA(Let-7a), as a tumor suppressor, in Ewing's Sarcoma cell lines. Methods To detect the Let-7a expression of different cell lines through Northern Blot；Constructing pMIR-reporter-CDK6 wild-type (CDK6_WT) or mutant (CDK6_MUT) and positive control (Let-7a_PC) plasmid; And then SK-ES-1 cells were transfected with Let-7a scramble, mimic and mimic control, The target gene of Let-7a was identified by using bioinformatics analysis combined with Dual-luciferase reporter and Western blot analysis. Results Northern Blot results showed the low expression of Let-7a in Ewing sarcoma cell lines, Grey mean of Let-7a /U6 snRNA maximum point：SK-ES-1 0.193±0.024(P<0.05 compared with MSCs); Restrictive enzyme digestion and DNA sequencing analysis revealed that the plasmid was constructed successfully. Let-7a was successfully overexpressed in SK-ES-1 cells (P<0.01); Compared with mutant CDK6 (CDK6_MUT) and Let-7a_PC, Wild-type CDK6 (CDK6_WT) luciferase expression levels were significantly decreased(P<0.05); over-expression of Let-7a in SK-ES-1 cell lines inhibited CDK-6 mRNA expression and protein. Conclusion The target gene of the tumor suppressor microRNA (Let-7a) was identified in Ewing sarcoma cell lines and can lay the foundation for the further experiments.

SASH1 may Cross-talk ERK Signaling Pathways through MAP2K2 and MAP4K4

2014, 34(11):  1530-1536. 

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Objective To investigate the protein-protein interactions between the novel candidate tumor suppressor SAM and SH3 domain containing 1 (SASH1) with MAP2K2 and MAP4K4，respectively Methods Retrovirus vector of SBP-Flag- SASH1-pBABE-puro was generated by subcloning SASH1 gene into Xho? and Hpa? sites and transfected into HEK-293T cells for the construction of stable cells expressing exogenous SASH1 after puromycin selection. Exogenous fusion protein expression of Flag-SASH1 was identified by western blot. Pull-down assay, LC-MS/MS analysis and immunoprecipitation were performed to explore the key SASH1 binding proteins which might regulate the proliferation, apoptosis and metastasis of tumor cells. SASH1-siRNA1 and SASH1-siRNA2 was transfected into MDA-MB-231 cell lines, compared with blank group and Negativ - siRNA. Western blot detection the interference effect of SASH1 protein and P-ERK1/2 levels after72 hours.Results Recombinant plasmid of SBP-Flag-SASH1-pBABE-puro was successfully constructed and stably expressed in HEK-293T cells. SASH1 had binding with both MAP2K2 and MAP4K4. The SASH1-siRNA can efficiently block the expression of SASH1, and the expression of P-ERK1/2 was increased in group of SASH1-siRNA. Conclusion MAP2K2 and MAP4K4 is an important candidate binding proteins of SASH1 and SASH1 may cross-talk with ERK1/2 signaling pathways probably through the mediation of them to promote many cell processes including cell proliferation and migration.

Inhibitory effect of Recombinant NDV (rl-RVG) on nude mice model inoculating with Lung Adenocarcinoma A549

2014, 34(11):  1537-1542. 

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Objective To explore the effects of rl-RVG recombinant adenovirus on the proliferation of A549 lung adenocarcinoma tumor-bearing mice and its mechanism of apoptosis. Methods After having infected A549 lung adenocarcinoma tumor-bearing mice with rl-RVG, the tumor volumes were detected, RT-PCR were used to detect the expression of NDV and RVG gene,,Tunnel assay used to determine the rate of cell apoptosis in tumour paraffin section and western blot was applied to measure the expression of caspase、Bax and Bcl-2 proteins. Results After rl-RVG infection, the growth of tumor in rl-RVG group were inhibited effectively（P <0.05）； RT-PCR showed that RVG gene were expressed in rl-RVG group and NDV gene were expressed in both rl-RVG and NDV groups. Tunel showed that there were more tumor apoptosis in rl-RVG group（P<0.05）. Western blot showed that caspase-3,8,9 protein were highly expressed in both rl-RVG and NDV groups（P<0.01）. The grayscale ratio of bax/ bcl-2 was higher in rl-RVG group（P<0.01）. Conclusion The study demonstrated that recombinant rl-RVG successfully, and effectively inhibited the growth of A549 lung adenocarcinoma , which may work through inducing activating bax and caspase-depended apoptosis pathway.

Tenascin-C induces the differentiation of human bone marrow stromal cells into osteoblasts

2014, 34(11):  1543-1547. 

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Objective: To investigate the induction effect of Tenascin-c (TN-C) on differentiation of human bone marrow stromal cells (hBMSCs) into osteoblasts. Methods: hBMSCs were obtained by the whole bone marrow culture method, and cells of passages 6~10 were used for all experiments. Human BMSCs were treated with different concentration of recombinant TN-C (0,1,10,50,100nmol/L) and osteogenic differentiation was detected by staining with Alizarin Red S. RT-PCR was performed to detect the mRNA levels of alkaline phosphatase (ALP) and osteocalcin (OCN), ALP detection kits were used for the detection of ALP activity, and OCN radioactive immune kit was used for the determination of OCN content. Results: 1. Alizarin red staining showed that direct application of exogenous rTN-C dose-dependently triggered the differentiation and mineralization of osteoblasts; 2. rTN-C treatment significantly increased the ALP avtivity and OCN content, compared with control group. Conclusion: TN-C could induce the differentiation of human bone marrow stromal cells (hBMSCs) into osteoblasts.

Associations of single nucleotide polymorphism in has-miR-27a and has-miR-124a gene with gestational diabetes mellitus

2014, 34(11):  1553-1557. 

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Objective To explore whether single nucleotide polymorphism (SNP) of has-miR-27a gene rs895819 and has-miR-124a gene rs531564 were associated with development of gestational diabetes mellitus (GDM). Methods A total of 1719 pregnant women including 840 with GDM and 880 controls with normal glucose tolerance (NGT) and negative result of 50g glucose challenge test [GCT(-)] were recruited. SNP were genotyped using TaqMan allelic discrimination assays. The genotype and allele distributions of each SNP between GDM and control groups and the biochemical data among different genotypes were analyzed. Results 1) The CC, CT and TT genotype frequencies of SNP rs895819 differed significantly between GDM group and control group (3.1%、39.3%、57.6% and 7.1%、37.6%、55.3%) (P<0.05), and the effect remained in a recessive model（CC vs CT+TT）[P=0.001; OR 0.435(0.270, 0.702)]. Moreover, the allelic frequencies of C was significantly lower in GDM group than in controls (P<0.05). 2) The levels of fasting plasma glucose in pregnant with rs895819 CC genotype were significantly lower than those with CG+GG genotype. Conclusions The C allele of rs895819 SNP in has-miR-27a gene reduced the risk of GDM.

Increased expression of CD1c and CD1d in peripheral blood of SLE with positive anticardiolipin antibodies

2014, 34(11):  1558-1559. 

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Specific targeted inhibition from RNA interference to TNF-α expression of rabbit synovial cells

2014, 34(11):  1560-1561. 

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Isolation, primary culture and identification of rat pancreatic islet pericytes

2014, 34(11):  1562-1563. 

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Distribution of gene polymorphism of methylenetetrahydrofolate reductase and methionine synthase among Han women in Langfang city

2014, 34(11):  1564-1565. 

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Advance on the mechanism of notch signaling pathway regulation on glioma stem cells

2014, 34(11):  1566-1569. 

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Glioma stem cells play a crucial role in glioma recurrence and resistance to therapies. Notch signaling pathway was involved in glioma stem cells' forming and maintaining. Moreover, Notch activation was contributed to chemotherapy and radiotherapy of resistance in glioma. Therefore, the study on Notch signaling pathway of glioma stem cells has positive significance for the development of targeted therapies.

Betatrophin: a novel factor influences the glucose and lipid metabolism

2014, 34(11):  1570-1573. 

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Betatrophin is a novel factor which regulate the glucose and lipid metabolism. Human betatrophin is located at chromosomal position 19p13.2, and the murine gene is present on chromosome 9. This is a highly conserved gene. Human betatrophin protein shows 73% identity and 82% similarity to the murine protein. It is predominantly expressed in liver and fat, and plays a great role in glucose and lipid metaboslim.

Research progress in cell metabolism of glucose and lipid regulated by transcription factors via mTOR

2014, 34(11):  1574-1577. 

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The mammalian target of rapamycin(mTOR) is an important signal molecule in a variety of physiological mechanisms, including protein synthesis, cell proliferation, cell cycle, energy metabolism, autophagy, etc. It is increasingly apparent that mTOR deregulation occurs in cancer and metabolism disorders. The transcription factors of downstream of mTOR such as HIF1α, c-Myc, FoxO1, SREBPs, PPARγ/PPARα and TFEB play key roles in regulating the metabolism of glucose and lipid.

Research progress in mTOR-targeted therapy for hepatocelluar carcinoma

2014, 34(11):  1578-1581. 

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Aberrant mammalian target of rapamycin (mTOR) signaling has a critical role in the pathogenesis of hepatocellular carcinoma (HCC) and is a potential target. The effects of mTOR inhibitors used as single agent on HCC are limited due to immunosuppression and resistance induced by paradoxical activation of Akt. Combined use of mTOR inhibitors and other anticancer agents synergistically improves the therapy for HCC, and may be a promising therapeutic strategy.

Urate transporters are promising targets for hyperuricemic therapies

2014, 34(11):  1582-1585. 

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Renal proximal epithelial cells contain multiple urate transporters, which could be categorized into reabsorption related, excretion related and skeleton proteins by function. Urate transporter 1, glucose transporter 9 and ATP-binding cassette family G subfamily type 2 are the three most important transporters; while PDZK1 provides structural support for other transporters. These urate transporters are the linkage of hyperuricemia and other metabolic disorders, as well as the promising target of new urate lowering therapies.

The current status of robot-assisted partial nephrectomy for renal cell carcinoma

2014, 34(11):  1586-1589. 

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Robot-assisted partial nephrectomy (RAPN) and Laparoscopic partial nephrectomy (LPN) are major alternatives for the performance of nephron-sparing surgery (NSS) in renal cell carcinoma minimally invasive treatments. With the development of instrument and technology as well as accumulation of experience, numerous clinical trials have shown favorable outcomes for RAPN.

Different effects of basic medical knowledge and PBL on comprehensive abilities of undergraduate students

2014, 34(11):  1590-1593. 

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Objective To reveal the different effects of basic medical knowledge and PBL on comprehensive abilities of undergraduate student, and provide evidence for medical education reform. Methods Choose one case aimed at Hepatitis B virus (HBV), compare the scores of Microbiology, PBL scores and scores of final exam on case. Results Students with higher Microbiology scores got higher scores on scenario-related multiple choice question (MCQ-S) compared with students with lower Microbiology scores (P < 0.05). Students with higher PBL scores got higher scores on short answer question (SAQ) compared with lower PBL scores (P < 0.05). Conclusion Basic medical knowledge and PBL have different effects on comprehensive abilities of undergraduate students.

Exploration and thinking on the reform of pharmacological experiment teaching for eight-year program medical education

2014, 34(11):  1594-1596. 

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Some reforming measures are proposed for the pharmacological experiment teaching in eight-year program medical education which consists of strengthening the training of the basic pharmacological experiment skills and methods, reducing the traditional verification experiments, increasing the comprehensive experiments, carrying out the designing experiments and improving the assessment methods. These measures might stimulate students’ interest and initiative, improve their manipulative ability, innovative ability and research capability, and are also the explorations for the experimental teaching reform in order to cultivate high quality medical talents with creative thinking and enhance the teaching quality.