Abstract: Objective To explore whether single nucleotide polymorphism (SNP) of has-miR-27a gene rs895819 and has-miR-124a gene rs531564 were associated with development of gestational diabetes mellitus (GDM). Methods A total of 1719 pregnant women including 840 with GDM and 880 controls with normal glucose tolerance (NGT) and negative result of 50g glucose challenge test [GCT(-)] were recruited. SNP were genotyped using TaqMan allelic discrimination assays. The genotype and allele distributions of each SNP between GDM and control groups and the biochemical data among different genotypes were analyzed. Results 1) The CC, CT and TT genotype frequencies of SNP rs895819 differed significantly between GDM group and control group (3.1%、39.3%、57.6% and 7.1%、37.6%、55.3%) (P<0.05), and the effect remained in a recessive model（CC vs CT+TT）[P=0.001; OR 0.435(0.270, 0.702)]. Moreover, the allelic frequencies of C was significantly lower in GDM group than in controls (P<0.05). 2) The levels of fasting plasma glucose in pregnant with rs895819 CC genotype were significantly lower than those with CG+GG genotype. Conclusions The C allele of rs895819 SNP in has-miR-27a gene reduced the risk of GDM.

Key words: gestational diabetes mellitus, single nucleotide Polymorphism, miRNA