Advance of circRNA in the research on orthopedic diseases

doi:10.16352/j.issn.1001-6325.2025.03.0399

Abstract

Abstract: Circular RNAs(circRNAs) are a class of closed-loop non-coding RNAs(ncRNAs) formed by head-to-tail covalent bonds. CircRNAs participate in the development of osteoarthritis by regulating extracellular matrix degradation, inflammatory response and homeostasis of chondrocytes; It regulates the osteogenic differentiation of bone marrow mesenchymal stem cells, the activity of bone microvascular endothelial cells and angiogenesis to affect femoral head necrosis; It regulates the differentiation of bone marrow stem cells, osteoblasts and osteoclasts to cause osteoporosis and it affects the invasion, metastasis and drug resistance of osteosarcoma through different pathogenesis. CircRNAs are expected to become a new clinical diagnostic marker in the future.

Key words: circular RNA, orthopedics diseases, miRNA sponge

CLC Number:

R681

LYU Shugang, ZHAO Tong, CHEN Guoliang, DU Xin, WANG Wenji. Advance of circRNA in the research on orthopedic diseases[J]. Basic & Clinical Medicine, 2025, 45(3): 399-403.

References

[1] Zhu J, Luo YM, Zhao Y, et al. CircEHBP1 promotes lymphangiogenesis and lymphatic metastasis of bladder cancer via miR-130a-3p/TGFβR1/VEGF-D signaling[J]. Mol Ther, 2021, 29: 1838-1852.
[2] Wang ZW, Dai QG, Song JM, et al. Predicting RBP binding sites of RNA with high-order encoding features and CNN-BLSTM hybrid model[J]. IEEE/ACM Trans Comput Biol Bioinform, 2022, 19: 2409-2419.
[3] Zhang CP, He WJ. Circ_0020014 mediates CTSB expression and participates in IL-1β-prompted chondrocyte injury via interacting with miR-24-3p[J]. J Orthop Surg Res, 2023, 18: 877. doi: 10.1186/s13018-023-04370-8.
[4] Ouyang X, Ding YZ, Yu L, et al. Circular RNA CircDHRS3 aggravates IL-1β-induced ECM degradation, apoptosis, and inflammatory response via mediating MECP2 expression[J]. Inflammation, 2023, 46: 1670-1683.
[5] Cheng S, Nie Z, Cao J, et al. Circ_0136474 promotes the progression of osteoarthritis by sponging mir-140-3p and upregulating MECP2[J]. J Mol Histol, 2023, 54: 1-12.
[6] Chang Q, Li C, Hu JZ, et al. Protective effects of hsa_circ_0072568 on interleukin-1β-stimulated human chondrocytes are mediated via the miR-382-5p/TOP1 axis[J]. Exp Ther Med, 2023, 26: 383. doi: 10.3892/etm.2023.12082.
[7] He AX, Liu YR, Zhang RB, et al. CircSFMBT2-OA alleviates chondrocyte apoptosis and extracellular matrix degradation through repressing NF-κB/NLRP3 inflammasome activation[J]. Heliyon, 2023, 9: e17312. doi: 10.1016/j.heliyon.2023.e17312.
[8] Wen XZ, Fang GB, Li HY, et al. CircIRAK3 exerts negative feedback regulation on inflammation by binding to HNRNP U and destabilizing proinflammatory cytokine mRNA in osteoarthritis and chondrogenesis[J]. Int J Biol Macromol, 2024, 256(Pt 2): doi:10.1016/j.ijbiomac.2023.128453.
[9] Feng XB, Xiang Q, Jia J, et al. CircHGF suppressed cell proliferation and osteogenic differentiation of BMSCs in ONFH via inhibiting miR-25-3p binding to SMAD7[J]. Mol Ther Nucleic Acids, 2022, 28: 99-113.
[10] Han N, Qian F, Niu XP, et al. Circ_0058792 regulates osteogenic differentiation through miR-181a-5p/Smad7 axis in steroid-induced osteonecrosis of the femoral head[J]. Bioengineered, 2022, 13: 12807-12822.
[11] Hao Y, Lu C, Zhang B, et al. CircPVT1 up-regulation attenuates steroid-induced osteonecrosis of the femoral head through regulating miR-21-5p-mediated Smad7/TGFβ signalling pathway[J]. J Cell Mol Med, 2021, 25: 4608-4622.
[12] Mao Z, Liu G, Xiao GY, et al. CircCDR1as suppresses bone microvascular endothelial cell activity and angiogen-esis through targeting miR-135b/ FIH-1 axis[J]. Orthop Surg, 2021, 13: 573-582.
[13] Yin P, Xue Y. CircRNA hsa_circ_0006859 inhibits the osteogenic differentiation of BMSCs and aggravates osteoporosis by targeting miR-642b-5p/miR-483-3p and upregulating EFNA2/DOCK3[J]. Int Immunopharmacol, 2023, 116: 109844. doi:10.1016/j.intimp.2023.109844.
[14] Wu J, Li X, Nie HP, et al. Phytic acid promotes high glucose-mediated bone marrow mesenchymal stem cells osteogenesis via modulating circEIF4B that sponges miR-186-5p and complexes with IGF2BP3[J]. Biochem Pharmacol, 2024, 222: 116118. doi: 10.1016/j.bcp.2024.116118.
[15] Zhou JX, Sui MZ, Ji F, et al. Hsa_circ_0036872 has an important promotional effect in enhancing osteogenesis of dental pulp stem cells by regulating the miR-143-3p/IGF2 axis[J]. Int Immunopharmacol, 2024, 130: 111744. doi: 10.1016/j.intimp.2024.111744.
[16] Zhang P, Liu J, Chai ZJ, et al. CircZfp644-205 inhibits osteoblast differentiation and induces apoptosis of pre-osteoblasts via sponging miR-455-3p and promoting SMAD2 expression[J]. Eur J Med Res, 2024, 29: 315. doi: 10.1186/s40001-024-01903-7.
[17] Tang L, Yuan L, Yan JY, et al. Circ_0029463 promotes osteoclast differentiation by mediating miR-134-5p/Rab27a axis[J]. J Orthop Surg Res, 2024, 19: 128. doi: 10.1186/s13018-024-04610-5.
[18] Smeland S, Bielack SS, Whelan J, et al. Survival and prognosis with osteosarcoma: outcomes in more than 2000 patients in the EURAMOS-1(European and American Osteosarcoma Study) cohort[J]. Eur J Cancer, 2019, 109: 36-50.
[19] 依马木买买提江·阿布拉, 帕哈尔丁·白克热, 易超,等.血浆hsa＿circ＿005230在原发性肝细胞癌诊断和预后评估中的临床价值[J]. 基础医学与临床,2023,43: 1417-1422.
[20] 郑彬彬, 林淑莹, 李德隆,等.环状RNA ATP2B1靶向miR-452-5p降低胃癌细胞系的增殖和侵袭[J]. 基础医学与临床,2023,43: 882-888.
[21] Shi YB, Tian YY, Wu YQ, et al. CircTNPO1 promotes the tumorigenesis of osteosarcoma by sequestering miR-578 to upregulate WNT5A expression[J]. Cell Signal, 2023, 111: 110858. doi: 10.1016/j.cellsig.2023.110858.
[22] Cai PF, Fu X, Li XF, et al. Upregulation of circ_0076684 in osteosarcoma facilitates malignant processes by mediating miRNAs/CUX1[J]. J Orthop Surg Res, 2024, 19: 260. doi: 10.1186/s13018-024-04742-8.
[23] Liu Y, Jiang H, Hu K, et al. CircPRMT5 promotes progression of osteosarcoma by recruiting CNBP to regulate the translation and stability of CDK6 mRNA[J]. PLoS One, 2024, 19: e0298947. doi: 10.1371/journal.pone.0298947.
[24] Zhang Y, Zhan YZ, Liu ZY, et al. Circ_0002669 promotes osteosarcoma tumorigenesis through directly binding to MYCBP and sponging miR-889-3p[J]. Biol Direct, 2024, 19: 25. doi: 10.1186/s13062-024-00466-1.
[25] Jiang X, Guo S, Wang S, et al. EIF4A3-induced circ-ARHGAP29 promotes aerobic glycolysis in docetaxel-resistant prostate cancer through IGF2BP2/c-Myc/LDHA signaling[J]. Cancer Res, 2022, 82: 831-845.
[26] Shen S, Xu Y, Gong Z, et al. Positive feedback regulation of circular RNA hsa_circ_0000566 and HIF-1α promotes osteosarcoma progression and glycolysis metabolism[J]. Aging Dis, 2023, 14: 529-547.
[27] Zhang D, Guo Q, You K, et al. M(6)A-modified circARHGAP12 promotes the aerobic glycolysis of doxorubicin-resistance osteosarcoma by targeting c-Myc[J]. J Orthop Surg Res, 2024, 19: 33. doi: 10.1186/s13018-023-04502-0.