Table of Content

25 October 2008, Volume 28 Issue 10

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研究论文

Mesenteric lymph duct ligation against hepatic injury subjected to two-hit in rats

Geng ZHANG; Jun-Xu REN; Zi-gang ZHAO; Yu-ping ZHANG; Chun-Yu NIU; Jing ZHANG

2008, 28(10):  0-0. 

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Objective To explore the mechanism of mesenteric lymph duct ligation against hepatic injury in rats by two-hit of hemorrhage and LPS. Methods 45 Wistar rats were divided into three groups: the ligation group, the non-ligation group and sham group, and the two-hit model was established by hemorrhage and LPS, mesenteric lymph was blocked by ligating mesenteric lymph duct in ligation group. After 24 hours of operation trauma, taken out the liver making for pathological section, and the hepatocellular apoptosis rate was determined by method of TUNEL, the expression of BCL-2 and BAX protein were determined by immunohistochemical test. At the same time, taken out liver making for homogenate of 10 percent, the activity of MPO and ATPase and the contents of TNFα and IL-6 were determined in hepatic homogenate. Results After two-hit, the hepatocellular apoptosis rate and expression of BAX protein in non-ligation group were significantly increased compared with sham group and ligation group, and expression of BCL-2 protein was significantly lower respectively. The contents of MPO, TNFα and IL-6 in hepatic homogenate of non-ligation group were significantly increased than that of sham group, and the activity of ATPase in hepatic homogenate was significantly lower. But the ATPase in hepatic homogenate of ligation group were significantly increased than that of non-ligation group, and the MPO, TNFα and IL-6 in hepatic homogenate of ligation group were significantly lower compared with non-ligation group. Conclusion The results demonstrated that the mechanism of mesenteric lymph duct ligation lightens the hepatic injury of rats was relating to the mesenteric lymph blockage reduces the TNFα and IL-6 and improves the expression of BCL-2 protein and the activity of ATPase in liver.

Alternariol enhanced DNA Polymerase β Expression in NIH3T3 Cells

Ji-min ZHAO; Ge JIN; Pei LI; Ming-yao ZHAO; Hong-yan YANG; Zhi-min ZHENG; Zi-ming DONG

2008, 28(10):  0-0. 

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Objective To study the effects of Alternariol (AOH) on DNA polymerase β (DNA polβ)expression in NIH3T3 cells. Methords RT-PCR and Immunocytochemistry and Western blot were used to detected mRNA and the protein levels of DNA polβ in NIH3T3 cell line induced by AOH. Results The expression of DNA polβ in NIH3T3 cells contaminated by AOH was significantly higher than that in the control group(P<0.05). Conclusion AOH induces overexpression of DNA polβ in the NIH3T3 Cells, which could be responsible to the DNA damage in cells caused by AOH.

Knockdown of the NF-κB signaling pathway by siRNA inhibits the proliferation and invasiveness of HeLa229 cell line

Wei-hong TIAN; Fang TIAN; Pei-rong XU; Hong-tao LIU; Le-xun XUE

2008, 28(10):  0-0. 

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Aim: To investigate cell proliferation and invasiveness of cervical cancer HeLa229 cell after inhibition of the NF-κB signaling pathway by p65siRNA. Methods: RNA interference was employed for specific inhibition of the expression of p65. The HeLa229 cell was divided into transfected group and untransfected group. Cell viability was detected by MTT after the HeLa229 cells were transfected with or without p65siRNA for 24, 48, 72h. The sensitivity to 5-Fu of the HeLa229 cell, transfected with or without p65siRNA, was evaluated also by MTT. Boyden chamber experiment in vitro was used to detect the invasion ability of HeLa229 cell. Results: p65 siRNA inhibited the cell proliferation as compared with the untransfected cells. Proliferations of both cells transfected with and without p65 siRNA were inhibited in a concentration-dependent manner, while at the same concentration of 5-Fu the viability of transfected HeLa229 cells was significantly suppressed (P <0.05). Compared with the untransfected cells, the number of cells which traversed through Matrigel was decreased obviously. Conclusion: RNAi targeting of p65 has anti-proliferative effects, inhibits the invasiveness and increases the 5-Fu sensitivity of the ESCC cells, suggesting that NF-κB might be a good target for cancer treatment.

HDAC7 gene is involves in atherosclerosis induced by intermittent hypoxia and hyperlipemia in rabbits

Yue-tao WU; Rui-hong LIU; Gu-xiang HUANG; Xiang-yu ZHANG; Yu YANG

2008, 28(10):  0-0. 

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Objective To investigate gene expression patterns of atherosclerosis (As) induced by intermittent hypoxia and hyperlipemia. Methods Isolation total mRNA of liver tissue from atherosclerosis rabbit model in hyperlipemia group and hyperlipemia with intermittent hypoxia group. Suppression subtractive hybridization was adopted to construct cDNA lib, and to identify differential expression by opposite Northern blot, after insertion element was sequenced blast analysis by quantitate RT-PCR. The expression of histone deacetylase 7 was obviously difference in two groups. Results MYL6 and HDAC7 were screened in cDNA lib. Conclusion The process of atherosclerosis induced by intermittent hypoxia is correlated to many genes. HDAC7 is a gene play new important role in AS.

Correlation between unexplained oligospermatism and azoospermatism and micro-deletion in AZF gene

Xiang-gai ZHANG; Hai-yan HUANG; Bang-rong ZHAO; Xiu-juan DONG; Jie LIU; Jun-rong YU; Feng-qin TAN

2008, 28(10):  0-0. 

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Objective To investigate the association between oligospermatism and azoospermatism in male sterility patients and micro-deletion in AZF gene of Y chromosome and to establish a integrate clinical diagnostic method for screening micro-deletion in AZF gene in Chinese. Methods PCR method was used to detect micro-deletion in AZF gene in 62 oligospermatism and azoospermatism patients and 20 normal male controls. Results 13.64%(6/44) of oligospermatism patients and 22.22%(4/18) of azoospermatism patients presented micro-deletion. However, micro-deletion was not found in 20 normal male controls. Furthermore, micro-deletion occur mostly in AZFc region. Conclusion Micro-deletion in AZF gene of Y chromosome is one of the major risks for oligospermatism and azoospermatism.

Phenytoin promotes Collagen Deposition and Activity of Matrix Metalloproteinases in Ventricle in rat after Experimental Myocardial Infarction

Yong ZHU; Yu-ming LI; Xin ZHOU

2008, 28(10):  0-0. 

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Object The purpose of this work is to investigate the possible mechanisms underling this phenomenon that phenytoin can accelerate the healing process by dynamic measurement of matrix metalloproteinase-2 and -9 activity and collagen changes in heart tissue in the early period after myocardial infarction in rat. Methods Adult male Wistar rats that survived ligatation of the left coronary artery were randomized to phenytoin group or operation control and compared to sham-operated rats. The time-dependent proteolytic activity of MMP-2 and MMP-9 were detected by gelatin zymography serially. Picrosirius red staining plus polarized light micrscopy was used for qualitative and quantitative analysis of collagen include collagen volume fraction (CVF) and ratio of typeⅠ/Ⅲ collagen. In addition, Infarct thickness and myocyte cross-sectional area were also evaluated by image analysis. Results 14 days after myocardial infartion (MI), phenytoin could reduce infarct wall thinning. Compared with control group, the rats received phenytoin had less myocyte cross-sectional area. 2 weeks after MI, CVF in two groups both had significantly dynamic increase and phenytoin could accelerate the beneficial change. In contrast to control group, ratio of typeⅠto type Ⅲ collagen in phenytoin increased more quickly. Apart from these results, phenytoin did little to CVF in non-infarcted region. Analysis of MMPs activity in myocardial extracts by zymography demonstrated that infarction-induced expression of proMMPs and active MMPs was both upregulated in phenytoin group and operation control. We found that after MI, MMP-9 activity increased as early as 1 day and reached a maximum then gradually descended, whereas MMP-2 started to increase rapidly and remain elevated for up to 14 days thereafter. Phenytoin seemed to enhance expression of MMP-2 and MMP-9. 1 day after MI, active MMP-9 in phenytoin group expressed an increasing trendency compared to MI control. Conclusion Phenytoin can attenuate the degree of post-infarction left ventricular dilation and expansion of the infarct during the early phase of MI healing. MMP-2 and MMP-9 enhanced by phenytoin probably played a prominent role.

A clinical trail of probiotics in treatment of IBS-D

Li-ming ZHU; Mei-yun KE; Li-ya ZHOU; Yao-zong YUAN; Jin-yan LUO; Xiao-hua HOU; Min-hu CHEN

2008, 28(10):  0-0. 

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Objective: To evaluate efficacy and safety of Medilac-S (Bacillus subtilis and Enterococcus faecium) and BIFICO (Bifidobacterium longum, lactobacillusacidophilus, Enterococcus fecalis) and to assess the therapy influence on quality of life (QQL) in patients with IBS-D. Methods: 158 patients with IBS-D fulfilling Rome II criteria were enrolled the study. Following one week screening, patients were randomly allocated to receive either Medilac-S or Bifico 2 tab. tid for two weeks and then followed up one more week. Symptoms were recorded, including abdominal pain and bloating, frequency of defecation, form of stools, and the sensation of urgency evacuation. QQL was surveyed by questionnaire. Results: 158 patients (101 male and 57 female, mean age 44±12 years) concluded the study. The courses of disease and symtom severity in two groups were comparable at baseline. Each symptom score and total symptoms score were decreased significantly after treatment in both groups (P<0.05). The total effective rate was 41.8% and 49.6% at the end of 1-week and 51.9% and 65.8% at the end of 2-week. The score of QQL reduced from 33.12±23.11 to 18.06±18.73 and 31.88±20.17 to 19.93±17.43, respectively (P<0.01). No No severe adverse events occurred except (3/79) and 4/79) mild adverse events have been recorded but nobody excluded from the study. Conclusion: Our study shows both Medilac-S and Bifico alleviated the systoms of IBS-D and improved the QQL of patients. They will be an effective and safety therapy choice for IBS-D.

Arsenic trioxide inhibition of the phosphorylation of P27kip threonine residue 187 in human hepatic carcinoma cell

You WANG; Mu-dan LU; Peng LI; Xiao-peng CUI; Ai-guo SHEN

2008, 28(10):  1009-1014. 

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Objective To investigate the relationship between growth inhibitory effect of arsenic trioxide(As2O3) and phosphorylation of P27kip threonine residue 187(P27T187) in human hepatocellular carcinoma(HCC) cell line SMMC-7721. Methods Cultured in vitro, SMMC-7721 were treated for 72h with 2μmol/L As2O3. The cell growth inhibition was detected by cell number counting and the cell cycle was detected by flow cytometry(FCM).The expression and localization of P27, T187 phosphorylated P27(p-P27T187) were detected by Subcellular Fractionation , Western blot and immunoflurescence. Results As2O3 significantly inhibited the proliferation of SMMC-7721 cell and cell cycle was arrested in G2/M. A striking decrease in p-P27T187 expression and a reciprocal increase in P27 expression were found in 2μmol/L As2O3-treated SMMC-7721 cell. Meanwhile, As2O3 decreased the protein levels of Cdk2 and cyclinE .The location of P27 was transferred from cytoplasm to nuclei and the expression of p-P27T187 was decreased in nuclei. Conclusion As2O3 inhibit the phosphorylation of P27T187, thereby promoting P27 accumulation in SMMC-7721 cell nuclei, inducing cel1 cycle arrest and growth inhibition.

Regulations of APOE on TLR signal pathways in RAW264.7 cells

Shu-guang YANG; Qian PENG; Yin-yan YU; Bin ZENG; Guo-hui JIAO; Yuan ZHANG; Rong-cun YNAG

2008, 28(10):  1015-1020. 

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Objective To determine the effects of APOE on the intracellular signal pathways mediated by different kinds of TLR. Methods We first cloned APOE gene, set up APOE tranfected stable cell line; and then detected the activity of both NF- B and AP-1 transcription factors via chemiluminescence method. Using flow cytometric analysis, we finally investigated the expression of surface molecules in the APOE transfected RAW264.7 upon exposing to LPS, PolyI:C, PGN and BDNA. Results We demonstrated that APOE not only affected the activity of NF- B and AP-1 but also regulated the expression of costimulatory molecules induced by different kinds of TLR ligands. Indeed, APOE transfected RAW264.7 showed the higher activity of NF- B and AP-1 upon exposure to LPS and polyI: C as compared with the control(p<0.05) although NF- B activity was little inhibited upon exposing to LPS（p<0.05）. While APOE transfected RAW264.6 were stimulated by PGN, PolyI:C, LPS and BDNA respectively, APOE could remarkably promote the expression of B7-H1 induced by PGN; whereas the expression of CD86, PD-1, CD11c and Gr1 were lower in these APOE transfected stable cells. However, APOE did not effectively affect the expression of these costimulatory molecules induced by LPS, BDNA and PolyI:C. Conclusion APOE could specifically affect the expression of costimulatory molecules mediated by PGN, and regulate the activity of NF- B and AP-1, implying that APOE might be involved in the regulation of TLR-mediated immune responses.

Effect of hydroxamate on blood pressure and vascular remodeling in rats

Xia ZHAO; Hong-fang JIN; Shu-xu DU; Yue CHEN; Chao-shu TANG; Jun-bao DU

2008, 28(10):  1025-1029. 

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Objective To investigate the effect of hydroxamate (HDX, inhibitor of endogenous sulfur dioxide production) on blood pressure and vascular structure in rat. Methods 4-week-old SHRs (spontaneously hypertensive rats) and WKY (Wistar-Kyoto) rats were divided into control and HDX-treated group (respectively, n = 8). Five weeks later, the blood pressure, SO2 content and GOT activity in serum and aorta, morphometric parameters, proliferative index (PI) and the expression of p-ERK were determined. Results Compared with WKY rats, the blood pressure, ratio of wall thickness to lumen radius, PI and the expression of p-ERK in aorta were increased, while the SO2 content and GOT activity in serum and aorta were decreased in WKY+HDX groups (P < 0.05 or P < 0.01). The SO2 content and GOT activity in serum and aorta of SHR were lower than WKY rat (P < 0.05 or P < 0.01). Conclusions Endogenous sulfur dioxide might play a pivotal role in maintaining normal pressure and vascular structure.

Nanometer transgenic technology on angiogenesis after myocardial infarction in rats

Hong-mei TANG; Rui-qing LIAN; Xiao-dong ZHANG

2008, 28(10):  1035-1039. 

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Objective To investigate the feasibility of the gene therapy of on angiogenesis after myocardial infarction in rats. Methods Thirty-six male SD rats, after the ligation of left anterior descending coronary artery, were divided into 2 groups at random, experimental group and control group. Expressions of VEGF were measured by RT-PCR and Immunohistochemistry (IHC). Angiogenesis and capillary density were evaluated by HE stain, and qualitative and quantitative analysis were carried out. The adverse effects were tested after treating pVEGF165-PLGA nanoparticle. Results Compared with control groups, ischemic myocardial cells persistently and stably expressed VEGF in experimental group; Vascular endothelial cells proliferated actively, and the effect of angiogenesis was significant; After treating 48 hours, nanoparticles were observed in myocardial cells. Conclusion Treating with pVEGF165-PLGA nanoparticle, it can stimulate effective host-derived angiogenesis, which results in the prevention of impaired cardiac muscle after myocardial infarction. It may be an effect way to transit gene to treat MI.

Clinical significance of antiendothelial cell antibody in systemic vasculitis and their autoantigens

Han-ping WANG; Wen-jie ZHENG; Fu-lin TANG; Yan ZHAO; Xin-ping TIAN; Hua CHEN

2008, 28(10):  1040-1043. 

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Objective: To investigate the prevalence, their autoantigen and the clinical significants of antiendothelial cell antibodies (AECAs) in systemic vasculitis. Method: Western blotting was performed to detect specific AECA in sera of systemic vasculitis, SLE, RA, SS and healthy donors. Then analyse the relationships of AECA with the disease manifestation. Result: (1) The prevalence of AECA was 77.7% in systemic vasculitis, 87.5% in SLE, 66.7% in SS, 7.14% in RA, and 10% in normal group, respectively. (2) AECA reacted with a heterogenerous series of endothelial proteins which ranged in molecular size feom 16 to 120kD. Furthermore, AECA against a 47kD endothelial cell antigen were more frequently found in a variety of systemic vasculitis and SLE. (3) Compared with those in AECA-negative patients, the mean levels of ESR in AECA-positive patients with TA and the mean levels of BVAS in patients with WG, MPA and CSS were both significantly higher in AECA-positive patients. Patients with BD who have AECA aganist 47kD endothelial cell proteins were more frequently found to have neuropathy than those 47kD-AECA-negative patients, and the prevalence of inhanced CRP are also more frequent. Conclusion: AECA showed to be correlated with the disease manifestation, and the same molecular size antigen could be found in a variety of systemic vasculitis and SLE.

Change of Na+ channel in chronic human atrial fibrillation

Li CHEN; Zhong-wen LI; Yan YANG; Zhi-fei LIU; Qi-yong LI; Xiao-rong ZENG

2008, 28(10):  1048-1051. 

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AIM: Investigate the changes of Na+ current characteristics and SCN5A expression in the human chronic atrial fibrillation (AF). METHODS: Na+ currents were recorded with the whole-cell patch clamp technique in single atrial myocyte of AF group and normal sinus rhythm group (SR group)．The sodium channel α-subunit gene (SCN5A) mRNA was measured by reverse transcription-polymerase chain reaction (RT-PCR). RESULTS: (1) neither current density nor time-dependent recovery of INa was altered by AF in human, but voltage-dependent inactivation of INa was shifted to more positive voltages in AF cells. (2) AF didn't affect mRNA concentration of SCN5A. CONCLUSION: INa is not the reason for the decrease of atrial conduction velocity in AF.

Differentiation of rats bone marrow mesynchymal stem cells into cardiomyogenic cells with pacemaking function

Xin WEI; Lian-feng CHEN; Bo-jiang LIU; Quan FANG

2008, 28(10):  1060-1065. 

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Objectives To find the way of inducing the bone marrow mesynchymal stem cells（MSCs）into cardiac cells with pacemaking function in vitro. Methods Dissociate the rat MSCs and induce them with 5AZA, bFGF+EGF, HGF, SCF, lysate of the sinoatrial cells respectively. The morphological changes were observed，and the expressing of protein cTnT，connexin 43 and HCN2/4 were analyse by immunohistologic and flowcytometry techniques.The pacmaking current If were evaluted by patch clamp techniques. Results All the methods can induce the bone marrow MSCs to differentiate into cardiac cells, which expressing cardiac cell specific protein and HCN2. The results shows that cells induced by 5AZA, bFGF+EGF and SAN CMs show higher rate of HCN2 expressing (22.9%,22.3%,11%). The cells of these groups have the pacmaking current If. Conclusions Lysate of the sinoatrial cells are the ideal methods of inducing the bone marrow MSCs to differentiate into cardiac cells with pacemaking function in vitro.HCN is a promising marker protein to select pacmaking cells out of the differentiated cells.

Relation of IL-18, collagen fibre and plaque stability in aorta atherosclerotic lesion

Yun LIU; Ren-kuan TANG; Jian-bo LI; Shi-xiong DENG

2008, 28(10):  1075-1078. 

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Objective To investigate relation of IL-18, collagen fibre(CF) and plaque stability in aorta atherosclerotic lesion. Methods morphology and content of CF in rupture of aorta atherosclerotic group(A group), non-rupture of aorta atherosclerotic group(B group) and normal aorta group(C group) by Massion's staining. Expression of IL-18 by immunohistochemical staining among three groups. Results Increasing and arranging turbulence of the CF was found in tunica intima, middle layer and tunica adventitia in A and B groups. Around necrosis of atheromatous plaque, the CF significantly increased and arranged turbulence, but the CF decreased or lacked in necrosis area of atheromatous plaque in A group. The CF significantly increased, arranged turbulence and extended to SMCs in necrosis of atheromatous plaque in B group. Arranging orderliness and no disruption of the CF was found in C group. Percent of CF content were significant difference among three groups (P <0.01). It showed the IL-18 strong positive in endothelial cells, inflammatory cells and necrosis of atherosclerotic plaques in A group, positive in B group and quite lowness or negative in C group. There were significant difference among three groups (P <0.05). IL-18 and CF were significant dependability among groups, respectively(P <0.05). Conclusions It was indicated IL-18 overexpression caused a marked decrease in collagen content and led to vulnerable plaque in aorta atherosclerotic lesion.

Value of Preprocedural Serum Levels of C-reactive Protein in Predicting Cardiovascular Events and Prognosis

Ely MUHAMMAT Shu-yang ZHANG

2008, 28(10):  1079-1082. 

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Objective To investigate whether the cardiovascular events and prognosis on non-coronary artery disease and coronary artery disease in 1 month and 6 months could be predicted by baseline levels of C-reactive protein (CRP). Methods Preprocedural serum levels of CRP were measured in 327 patients perpormed with CAG or PCI . the patients were divided into two groups: CRP<0.3mg/dl and CRP≥0.3mg/dl. The cardiovascular events and stent resternosis, re-PCI, CABG, tachycardia arrhythmia were carefully observed and recorded intraprocedural and in 1month and 6 month after the procedures. Results The baseline levels of CRP were significantly higher in the patients with acute coronary syndrome than those with stable angina pectoris and non- coronary artery disease (5.15 ± 0.40 vs 0.27±0.05, 0.26±0.35, P＜0.01). The frequency of cardiovascular events was significantly higher in acute coronary syndrome and high baseline levels of CRP than those with stable angina pectoris and normal baseline levels of CRP in 1 month (4.86% vs 2.44%, P＜0.05; 16.36% vs 3.70%, P<0.01), The cardio vascular events and those in in-stentresternosis,re-PCI,CABG, tachycardia arrhythmia were significantly high in the acute syndrome than those with normal baseline levels of CRP and stable angina pectoris in 6 months too（12.50% vs 6.10%, P<0.05; 26.06% vs 13.58%, P<0.01）.Conclusion Preprocedural CRP level may be a predictor in 1 month and 6 months outcome of patients undergoing CAD after PCI or non-CAD. It indicated that the complications and clinical resternosis in 6 months were markedly influenced by the preprocedual degree of inflammatory cell activation .

技术与方法

Lentiviral Vectors Mediated GFP Transfection and Human Embryonic Stem Cell Culture

Ning LI; Bao-chang ZHU; Wan-wan ZHU; Shu-yan WANG; Ping REN; Yun-qian GUAN; Yu ZHANG

2008, 28(10):  1083-1087. 

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Objective To establish a culture system for human embryonic stem cell and label the hES cells with GFP by lentivirus. Methods We typically cultured the human ES cells on the feeders or matrigel. Furthermore, we characterized the undifferentiated hES by their ability to form compact clones positive for stage specific embryonic antigen-3 and surface alkaline phosphatase staining. Results Human ES cells cultured both on feeder cell and matirgel expressed typical surface markers like SSEA-3 and alkaline phosphatase. After transfected by lentivirus and hygromycin selection, green fluorescent protein was strongly expressed in human ES cells. Conclusion we succeed in human ES cell culture with feeder cells and matrigel.And got human ES cells expressing GFP continuously.

Method for DNA isolation from the blood stored

Jiang-chao LI; Wen-li SHENG; Wang-wei CAI; Zi-hong YANG

2008, 28(10):  1088-1089. 

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Human umbilical vein endothelial cells as a feeder layer promote the growth of embryonic stem cells

Zhi-hua WANG; Zhi-xu HE; Qiang MI; Hao-wen WANG

2008, 28(10):  1090-1094. 

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Objective To investigate whether human umbilical vein endothelial cells as a feeder layer was capable of supporting the growth of embryonic stem cells in vitro. Methods Human umbilical vein endothelial cells were isolated and cultured and then prepared as feeder cells after 3 passages. Alkaline posphatase activity (AKP) staining, stem cell surface marker test and karyotypes were conducted during different periods of cell culture. The suspension of stem cells cultured after 20 passages on endothelial cells were inoculated to the legs of severe combined immunodeficiency (SCID) mice subcutabeously to test the teratoma formation. Results E14.1 embryonic stem cells retained good colonies when they were cultured on endothelial cells for 3 passages and 8 passages. In addition, they expressed SSEA-1, Oct-4, and a membrane alkaline phosphatase to a high extent at passages 3 and 8. Embryonic stem cells cultured for 15 passages stably retaind a normal karyotype. Embryonic stem cells cultured on endothelial cells for 20 passages were inoculated into the hind leg of SCID mouse, teratomas containing three embryonic layers were recovered six weeks later. Conclusion Human umbilical vein endothelial cells would support effectively embryonic stem cells expansion, and provide a clinically safe method to expand ES cells for future clinical application.

研究短文

Acylation stimulating protein activated the expression of downstream proteins in adipocytes and preadipocytes

Yu WEN; Hong-wei WANG; Xiu-fen HU; Hui XU; Jing WU; Hui-ling LU; Jun WEI

2008, 28(10):  1095-1096. 

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Hyperglucose inhibition of the growth of cultured Müller cells

Min-li HUANG; Wei-ping CHEN; Shao-jian HE; Fang CHEN; Guo-rong LUO

2008, 28(10):  1097-1099. 

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Role of endothelin-1 and angiotension II in a rat model of Cyclosporine A- induced chronic nephrotoxicity

Hai-ping WANG; Qiao-ling SUN; Gang LIU; Xue-gang LI; Xiang-hua HOU; Bing CHEN; Guang-ju GUAN

2008, 28(10):  1100-1102. 

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短篇综述

Recent progress of myocardin and its relation with cardiovascular diseases

Ping XIE; Hui-hua LI

2008, 28(10):  1103-1106. 

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Myocardin is a remarkably potent transcriptional coactivator that specifically expresses in cardiovascular system and binds directly to serum response factor. It activates transcription of a subset of SRF-regulated genes encoding cardiomyocytes and smooth muscle cells contractile proteins. Abnormal expression of myocardin is associated with cardiovascular diseases, including atherosclerosis, myocardial hypertrophy, hypertension. Here, recent developments in the structure, expression and regulation of myocardin and its relation with cardiovascular diseases were reviewed．

Oncoproteomics and its application

Xiao-li WEI; Yue WANG

2008, 28(10):  1107-1110. 

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Proteomic research first came to the fore with the introduction of two-dimensional gel electrophoresis and proteomics has been increasingly applied to cancer research with the wide-spread introduction of mass spectrometry and protein chip. As important research tools, oncoproteomics that targets the entire cancer-specific protein network has already been applied in cancer research .In this article the development of the oncoproteomics and its applying in cancer is reviewed.

Expression of Caveolin-1 in tumor and Clinical significance

Wei-jia DONG; Jian-guo ZHANG; Jing-wu YANG; Bo ZHANG

2008, 28(10):  1111-1116. 

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Caveolin-1 (Cav-1) is a scaffold protein of caveolae that acts as a tumor modulator by interacting with cell adhesion molecules and signaling receptors. Evidence from a variety of studies indicate that caveolin-1 exhibits the heterogeneity of Cav-1 expression in different tumors. Current research has clearly established a role for Cav-1 as a a novel prognostic marker, Future studies will undoubtedly offer novel exciting opportunities to develop anti-cancer therapies.