Abstract: Objective To study the effects of Alternariol (AOH) on DNA polymerase β (DNA polβ)expression in NIH3T3 cells. Methords RT-PCR and Immunocytochemistry and Western blot were used to detected mRNA and the protein levels of DNA polβ in NIH3T3 cell line induced by AOH. Results The expression of DNA polβ in NIH3T3 cells contaminated by AOH was significantly higher than that in the control group(P<0.05). Conclusion AOH induces overexpression of DNA polβ in the NIH3T3 Cells, which could be responsible to the DNA damage in cells caused by AOH.

Key words: DNA polβ, alternariol, NIH3T3 cells