Table of Content

05 February 2010, Volume 30 Issue 2

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研究论文

Increased Expression of Autoantibodies and Soluble IL-6 Receptor in Serum of Patients with Tourette's Syndrome

Yu-hang CHENG; Yi ZHENG; Li-fang WANG; Rui-mei LIU; Yang GAO

2010, 30(2):  113-116. 

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Objective: To explore the mechanism underlying the immunologic injury in TS patients. Methods: The serum levels of anti-brain antibody（ABAb）, antinuclear antibody（ANAb）, soluble IL-6 receptor (sIL-6R), and soluble gp130 (sgp130) in patients with TS were analyzed by commercialized ELISA kits,and the titer of anti-streptolysin O (ASO) in TS and control was examined with latex enhanced immunoturbidimetry. Results: The levels of sIL-6R and sgp130 were significantly elevated in TS compared with control group (44.1 15.8 vs 30.3 9.0, P<0.01；69.0 24.6 vs 47.3 14.1，P<0.01, respectively) . ASO titer ( 250IU/ml) were found higher in TS than that in control（P 0.01）. The positive rates of anti-brain antibody and antinuclear antibody in TS were higher than that in control group as well (66% vs 4% p 0.01; 53% vs 25%, p 0.01). The level of ABAb negatively correlated with sgp130 concentration (r= 0.375, p<0.05). Conclusion: IL-6 signal transduction might be involved in Tourette's syndrome to lead the function upward and start the network mechanism of feedback inhibition, because elevated levels of sIL-6R, sgp130，ANAb and ABAb in serum. Autoimmune-related injuries may participate in the pathogenesis of the disease.

Prokaryotic expression, purification and immunoreactivity of Mycobacterium tuberculosis Ag85A protein

Mao-zi DENG; Chun-wei SHI; Fang WANG; Rui-ling FU; Chun WANG; Zheng-ming FANG; Xiong-lin FAN

2010, 30(2):  117-121. 

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Objective In order to obtain the large amount of M. tuberculosis Ag85A protein by prokaryotic expression. Methods The fbpA gene encoding M. tuberculosis Ag85A protein was amplified by using polymerase chain reaction (PCR) from M. tuberculosis H37RV strain. The PCR product was cloned into prokaryotic expression vector pProEXHTb to generate the recombinant plasmid pProfbpA, which was then transformed into the competence Escherichia coli BL21 cells. The recombinant Ag85A protein was successfully expressed by being induced with isopropyl thio-β-D-galactoside (IPTG) and purified by the Ni-Purification System. The distribution of fbpA gene in different environmental mycobacterial strains was screened by PCR and ELISA was performed to determine the immunoreactivity of the recombinant Ag85A protein with sera from different mycobacterial infections. Results 32 kd Ag85A protein was successfully expressed and purified. It was confirmed by PCR and ELISA that fbpA gene presented in the genomes of M.tuberculosis H37Rv, H37Ra, BCG, M. smegmatis, M. terra, M. trivial and M. phlei, but being absent in the genomes of M. vaccae. There were the highest Ag85A antibody titers in serum of TB patients and mice which being infected by M. tuberculosis H37Rv. Conclusion The recombinant Ag85A protein was successfully expressed and purified.

Serum hydrogen sulfide reduced in idiopathic pulmonary fibrosis patients

Dan SUN; Li-ping FANG; Bin GENG; Xin-min LIU; Chao-shu TANG

2010, 30(2):  122-124. 

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Objective To explore the effect of endogenous H2S in Idiopathic Pulmonary Fibrosis(IPF) patients, and its relationship with the severity of IPF. Methods Plasma H2S in 27 patients with IPF（IPF group）and 28 healthy physical examination people (control group) were tested. PaO2，ESR，CRP，LDH，HRCT and lung function in IPF group were tested to evaluate the severity of the disease at the same time. Then the concentrations of H2S in aggravating and palliative period in IPF group were observed. Results ⑴ Compared with the control group, the concentration of plasma H2S in IPF group was reduced significantly (P﹤0.01). ⑵The concentration of H2S in aggravating period was higher than palliative period (P﹤0.01). ⑶With the progress of HRCT，there was a descending trend of plasma H2S (P>0.05). Conclusion Endogenous H2S was probably involved in the course of IPF, and was associated with the progress of the disease.

Effects of Transplantation of mesenchymal stem cells combining with ADM，HGF gene treat myocardial infarction in rats

Jin-zi SU; Mao-hai LIN; Mei-ping LIN

2010, 30(2):  125-132. 

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Objective To investigate Effects of Transplantation of mesenchymal stem cells combining with two different gene transfection on the treatment of myocardial infarction in rats. Methods MSCs were isolated and expanded using the preplating method. Ad-ADM or Ad-HGF expression in MSCs and its secretion in culture medium were measured by ELISA. The left anterior descending branch of rats was ligated to establish a myocardial infarction model. Then the pretreated MSCs were labeled by DAPI, and were injected directly into the infarcted myocardium at open thorax surgery. Four weeks later, cardiac function was evaluated using echocardiography. Hearts were harvested. Immunohistochemistry staining of factor Ⅷ was used to evaluate the density of capillary vessels in infarction area. Immunohistochemistry staining of Troponin I (TNI) and connexin 43 were used to evaluate the differentiation and expression of implanted MSCs. Fluorescence microscope was used to identify the DAPI-labeled cells. Results The expression of ADM or HGF was traced in culture medium and has the time-effect relationship. DAPI-labeled transplantion MSCs were founded in the hearts of the recipients and expressed TNI in all MSCs transplantation groups. Immunohistochemical studies demonstrated that intense immunostaining for connexin 43 was higher in Ad-ADM plus MSCs group and Ad-HGF plus MSCs group, compared with MSCs group. A combination of Ad-ADM or Ad-HGF trensfection and MSCs transplantation demonstrated a further increase in capillary density and further improved LVEF compared with MSCs alone. There is no statistics significance between Ad-ADM plus MSCs group or Ad-HGF plus MSCs group in all index. Conclusion Ad-ADM or Ad-HGF transfection enhanced the angiogenic potency of MSCs transplantation and improved cardiac function. The treatment of myocardial infarction in rats in both Ad-ADM plus MSCs group and Ad-HGF plus MSCs group is no statistics significance.

Ischemic postconditioning attenuates myocardial cell injury by ischemia-reperfusion injury in rabbits

Yan HE; Zhi-yu ZENG; Jin-yi LI; Guo-qiang ZHONG; Wei LI; Wei-ke LI; Hong-hong KE

2010, 30(2):  133-138. 

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Objective To investigate the effects of ischemic postconditioning on apoptosis and structural and functional changes of mitochondria induced by myocardial isehemia/reperfusion(I/R)injury of rabbits in vivo and potential mechanism. Methods Eighty healthy rabbits were divided randomly into five groups：sham operation group(Group Sham)，ischemic reperfusion group (Group IR)，ischemic preconditioning group (Group IP), ischemic postconditioning group (Group PC)and 5-HD plus ischemic postconditioning group (Group PC+5-HD)，with ten rabbits in each．All rabbits in the five groups were killed 4h after reperfusion．The heart was quickly removed to be used for observing the structure of mitoehondria and measurement of the apoptosis rate by TUNEL. We observed ultrastructural changes of myocardium under electron microscope and examined mitochondrial membrane potential and Ca2+ concentration, MDA content and SOD activity of myocardial mitochondria. Results Compared with group IR,the damage of mitoehondrial ultrastrueture were milder and the apoptosis rate were decreased and Ca2+ concentration and MDA content were much lower in group IP and group PC (P< 0.05)．Mitochondrial membrane potential and SOD activity of myocardial mitochondria in group IP and group PC was significantly higher than that in group IR(P

Relationship between human membrane associated sialidase(Neu3) and multidrug resistance (MDR) in K562 cell line

Li XU; Lian-kun SUN; Feng LI; Bin YU; Xiao-jie LI; Xue-jian ZHAO; Yang LI

2010, 30(2):  139-143. 

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Objective To investigate the role of human membrane associated sialidase (Neu3) in multidrug resistance (MDR) of K562 cells. Method K562 cells and K562/ADM cells were treated with DNR alone or the combination with DNR and NeuAC2en.The cells survival rate was measured by MTT assay; the expression of MDR related factors and apoptosis related factors, such as MDR, MRP, MGMT, GST, Bcl-xl, Bcl-2 and Bax were determined by western blot and RT-PCR; Neu3 activity was detected by TBA reaction. Result The survival rate of K562/ADM cells was higher than that of K562 cells; NeuAC2en showed synergistic effect with DNR(P<0.01); Neu3 activity of both K562 and K562/ADM cells decreased after DNR or NeuAC2en induction and the decrease degree was the most statistically significant in the combination treatment group(P<0.01). Under the identical condition, the protein expression of P-gp and Neu3 and the mRNA expression of MDR1, Neu3, Bcl-xl and Bcl-2 increased with comparison to K562 cells. The mRNA level of Bax in K562 and K562/ADM groups was not changed significantly. MDR1, Neu3,Bcl-xl and Bcl-2 decreased after DNR induction and down-regulated most obviously in the groups treated by DNR combined with NeuAC2en. Conclusion Neu3 may be related with multidrug resistance of K562/ADM cell through regulating apoptosis and the expression of MDR1.

Detection and analysis of genomic copy number variations in a 46,X0,+der(?) fetus by array-based comparative genomic hybridization

Yan-liang ZHANG; Yong DAI; Zhi-guang TU; Qi-yun LI; Jing-hui REN; Li ZHANG; Lin-qian WANG

2010, 30(2):  144-150. 

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Objective To understand genomic copy number variations (CNVs) and ascertain karyotype for a 46,X0,+der(?) fetus, and investigate possibility and superiority of array-based comparative genomic hybridization (array-CGH) in clinical cytogenetic diagnosis. Methods G-banded chromosome analysis was carried out. The whole genome of the fetus was scanned and analysed by array-CGH. The results of array-CGH were confirmed by RT-qPCR. Results G-banded chromosome analysis showed the fetal karyotype was 46,X0,+der(?). Array-CGH revealed the derivative chromosome was Y chromosome without CNVs. A total number of 118 submicroscopic CNVs were identified. Comparable results between array-CGH and RT-qPCR were obtained for 9 novel CNVs. Conclusion Comparing with conventional cytogenetic analysis, array-CGH is of high resolution, high-throughput and high accuracy, which provides a powerful plateform for accurate detection of submicroscopic chromosomal aberrations.

Anatomy of tensor fascia latae perforator flap and its clinical significance in reconstruction of head and neck defects

Yun FENG; Wen-ting LI; Nai-li WANG; Ping-zhang TANG; Zhen-gang XU; Bin ZHANG

2010, 30(2):  151-154. 

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Objective　 To study anatomy of the tensor fascia latae perforator flap (TFLP flap) and explore its clinical application in reconstruction of head and neck defects. Material and methods Five fresh cadavers were prepared, and morphosis and blood supply of TFLP flap were examined by microsurgery anatomy. During dissections, the following parameters were recorded: number and type of perforators vessels, diameter of perforators, pedicle length, diameter of the original vessels, course(infra fascia and supra fascia),and its position were located by anatomical landmark. Results There were 41 TFLP flap perforators in all specimen with 35 musculocutaneous perforator and 6 septocutaneous perforator. Original vessel was ascend branch of lateral circumflex femoral artery/vein with average diameter of 3.01±0.49mm/3.28±0.57mm. The mean pedicle length was 9.1±0.79cm. The surface location was 4.22±1.37cm laterally and 8.73±2.72cm beneath to anterosuperior iliac spine. Conclusion With the characteristics of constant position、large caliber and convenient preparation, TFLP flap is useful for operation and option in reconstruction of head neck defects and considered as backup of anterolateral thigh flap. The disadvantage of this flap is its short vascular pedicle.

Effects of Rapamycin on growth and mTOR/4EBP1 Signalling Pathway in Xenograft of Esophageal Squamous Cell Carcinoma

Gui-qin HOU; Shu-man LIU; Li-li WANG; Xin JIA; Li WANG; Le-xun XUE

2010, 30(2):  155-158. 

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Objective The purpose of this study was to investigate the effects of rapamycin on tumor growth and mTOR/4EBP1 signaling pathway in xenograft of esophageal squamous cell carcinoma (ESCC) EC9706 cells. The expressions of factors in mTOR/4EBP1 signaling pathway of the tumor tissues mentioned above were analyzed by RT-PCR and Western blotting, respectively. Results Both rapamycin and cisplatin significantly inhibited the growth of transplantable tumors compared to control group (P<0.05) and their combination had a stronger effect than that of them alone (P<0.01). The results of RT-PCR and Western blotting revealed that rapamycin significantly decreased the expression of mTOR and p-4EBP1 but increased the expression of 4EBP1. Conclusion Rapamycin inhibits the mTOR/4EBP1 signalling pathway, resulting in the growth inhibition of xenograft of EC9706 cells.

PLOD2 is Involved in Human Skin Fibrosis

Chun-hui ZHU; Zhong-jin LUO

2010, 30(2):  159-164. 

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Objective To discuss the relationship between the expression of PLOD2 and the formation of collagen I in the scar. Methods To detect the mRNA expression of PLOD2 and COLIα1 in the scar and normal skin tissue , as well as the content of COLIα1 by RT-PCR and western blot; design and synthesis three kinds of PLOD2 ASODN targeted against the translational initiation , the joint of the first and the second exons and termination codon respectively，transfected into NIH/3T3 mouse fibroblast cell and detect the mRNA expression of COLIα1 and formation of collagen I by RT-PCR and western blot .Results : the expression of PLOD2 in the scar tissue is significantly higher than that in the normal tissue（P<0.05）, three kinds of ASODNs exert inhibitory effect on the mRNA expression of COLIα1 and formation of collagen I in fibroblast（P<0.05）, during which the repressive efficiency of ASODN targeted against the translational initiation is the highest（P<0.001）; while the mRNA expression level of COLIα1 has no statistical difference in the scar tissue , normal tissue and the groups treated with three kinds of ASODN. Discussions the high expression of PLOD2 is tightly related to the formation of collagen I, PLOD2 ASODN can suppress the formation of collagen I post-transcriptionally.

Slienced TROP2 gene to inhibit the migration and invasion of gastric cancer BGC-823 cells

Dong-fang DAI; De-yu CHEN; Shi-he SHAO

2010, 30(2):  165-169. 

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Objective To investigate the role of TROP2 in migration and invasion of human gastric cancer cells. Methods Small interfering RNA（siRNA）targeting TROP2 gene was constructed by gene clone technology and transfected into gastric cancer cell line BGC-823. The expressions of mRNA and protein were detected by Real-time quantitative PCR and Western blot assay after RNA interference. The proliferation capability was determined by MTT assay. Transwell assay was performed to assess the effect of TROP2 targeted RNA interference on the migratory and invasive properties of gastric cancer in vit ro. Results 　Enzyme digestion analysis and DNA sequencing showed that TROP2 targeted RNA interference recombinant plasmids were constructed successfully. The most effective recombinant plasmid was selected. After transfection , knockdown of TROP2 significantly impaired the proliferation ，migration and invasion capability of BGC-823 cells in vit ro（P < 0. 05）.Conclusion 　Interfering and down-regulating TROP2 gene can inhibit migration and invasion of gastric cancer cell line BGC-823，indicating that TROP2 gene is probably a target for gastric cancer gene therapy.

Decreased expression of adiponectin and receptor in rats with alcoholic liver disease

Wei WANG; Jun-ying ZHOU; Cai-yan ZHAO; Ya-dong WANG

2010, 30(2):  170-174. 

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Objectives To investigate the expression and significance of adiponectin(Adip) and the it's receptor 1,2(AdipoR1, AdipoR2) in the pathogenesis of alcoholic liver disease(ALD). Methods 50 male Wistar rats were acclimatized for 7 days and then 10 rats were randomly assigned to the normal control group. Others were to develop the rats model of ALD by intragastric alcohol of increasing concentration gradually (30%-60%, 5-9g·kg-1·d-1), 8, 8, 8 and 9 rats were sacrificed randomly at the end of 4th, 8th , 12th and 16th week, liver samples and liver homogenate (10%) were collected respectively. The concentrations of triglyceride (TG) in the liver homogenate and the level of adiponectin and tumor necrosis alpha (TNFα) in the serum were measured by biochemical chromatometry and ELISA method respectively. The mRNA and protein expression of Adip, AdipoR1 and AdipoR2 in the hepatic tissue were performed by reverse transcription polymerase (RT-PCR) and western blot. Results The model of rats alchoholic liver disease was developed. With the progress of ALD，the level of TG and TNFα in serum increased and the contention of Adip decreased gradually. The expression of Adip and AdipoR2 mRNA and protein decreased and no influenced in the AdipoR1. There is negative correlation between the expression of serum Adip and AdipoR2 in hepatic tissue and the level of serum TNF-α and TG in the liver homogenate.（r=-0.98~-0.90, P<0.01）. And the expression level of Adip protein was positively correlated with the contents of Adip in the serum (r=0.90, P<0.01). Conclusions There is a close correlation between the decreased expression of Adip and AdipoR2 and adipose degenaration and inflammation in hepatic tissue.

Oncogene DEK cooperates with AP-2α transcriptional factor to promote HER2 overexpression in breast cancer

Qian-chuan HUANG; Jun-hao CAO; Meng JI

2010, 30(2):  175-178. 

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Objective To investigate the pathological significance of DEK-AP-2α interaction in HER2 overexpression and breast tumorigenesis. Methods The protein level of DEK，AP-2α and HER-2 in breast tumor tissues was detected by Western blot. The interaction of DEK and AP-2α in MDA-MB-453 mammary cancer cells was detected by immuno-coprecipitation. Furthermore the impact of DEK and AP-2α on HER2 expression was investigated by siRNA in MDA-MB-453 mammary cancer cells followed by semi-quantitive RT-PCR and Western blot. Resluts A correlation between DEK, AP-2α and HER2 levels in breast cancer tissues were observed. The interaction between DEK and AP-2α in MDA-MB-453 cells was verified by co-immunoprecipitation assay. Depletion of DEK and AP-2α in MDA-MB-453 cell by siRNA cooperatively repressed HER2 expression at both the mRNA and protein levels. Conclution This study provides oncogene DEK cooperates with AP-2α transcriptional factor to promote HER2 overexpression in breast cancer.

Effects of Monoclonal Antibodies Against Human Stathmin 1 Combined vinblastine on Proliferation of K562 Cell Lines

Shuang WANG; Xiao-li LIU; Shao-fei YUAN; Hong-yan DU

2010, 30(2):  179-184. 

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Objective To investigate the effects of monoclonal antibodies against stathmin 1 combined vinblastine on the proliferation of K562 cells. Methods K562 cells were treated with monoclonal antibodies against stathmin 1, vinblastine alone or their combination , with the untr- eated cells used as the control, 24,48, 72,96hours later, inhabitation rate was studied by MTT assay ; The apoptosis was analyzed by invert microscope and also by flow cytometry with Annexin V/PI.Results The quantity decreased and shape, size changed after treating with different concentration of experimental groups. Monoclonal antibodies against stathmin 1 and vinblastine used alone or in combination both inhibited the proliferation of K562 cells，the inhibition ratio of their combination is more higher(P<0.05),and a synergistic effect of the two agents was noted in their combined action (P<0.05). Combined treatment of the cells resulted in significantly higher apoptsis rate than that in the other groups (P<0.05). Conclusion Monoclonal antibodies against stathmin 1 and vinblastine used alone or in combination both can inhibite proliferation of K562 cells and induce apoptsis. A synergistic effect is observed between the monoclonal antibodies against stathmin 1 and vinblastine in their inhibition of K562 cell proliferation.

Culture Supernatant of Toxoplasma gondii Inhibits The Proliferation of Human Lung Cancer Cell Line A549

Pu GE; Chun-li LI; Ya-lin RAN; Bin YE

2010, 30(2):  185-188. 

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Objective: To investigate whether there is inhibition of the human lung cancer cell line A549 induced by the culture supernatant of Toxoplasma gondii in vitro and the mechanism of the inhibition. Methods A549 cells 5×104mL-1 were cultured and harvested in the different cell culture plates. The cells were treated for different hours with different concentrations of Toxoplasma gondii culture supernatant (the concentrations of tachyzoites were 4×107mL-1, 8×107mL-1, 16×107mL-1 respectively). Growth inhibition rates were measured with the MTT method；Cell cycle was checked with Flow Cytometer. Western blot was used to detect the leves of cyclinB1、cdc2 of cells. Results The culture supernatants of Toxoplasma gondii could inhibit the proliferation of A549 cells in a time-dose dependent manner. Cell cycle was significantly stopped at G2/M phase by the culture supernatants with FCM technology. The culture supernatant of Toxoplasma gondii reduced the expressions of gene cyclinB1and cdc2 of A549 cells. Conclusion The culture supernatant of Toxoplasma gondii may inhibit A549 cell and arrest the cell cycle of A549 cells mainly by regulating the expression of gene cyclinB1 and cdc2

临床园地

Clinical Analysis of 45 cases of Hemophagocytic Syndrome

Jie MA; Wen-jie ZHENG; Xuan ZHANG; Fu-lin TANG

2010, 30(2):  189-193. 

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Objective To summarize the clinical features of patients with hemophagocytic syndrome (HPS). Methods The underlying diseases, clinical characteristics, laboratory findings, responses to therapy as well as their outcomes of 45 patients with HPS were analyzed retrospectively. Results The underlying diseases included virus infection(n=9), tuberculosis infection(n=2), rheumatic diseases(n=4), malignancies(n=22), unknown origin(n=8). HPS was clinically characterized by high fever(93.3%), hepatomegaly (77.8%), splenomegaly(80%), lymphadenopathy(55.6%), skin rash(24.4%), gastrointestinal hemorrhage(22.2%), renal (35.6%) and central nervous systern involvement(15.6%), five patients presented with disseminated intravascular coagulation(DIC)(11.1%). Laboratory data mainly manifested with cytopenia(100%), liver dysfunction(77.8%), hypofibrinogenemia(62.8%), hypertriglyceridemia (71.1%) , serum ferritin>500μg/L(87.5%), low NK-cell activity(92.9%) and hemophagocytosis in bone marrow(100%). Based on treating underlying diseases and use of corticosteroids and immunosuppressive agents in combination with intravenous immunoglobulins(IVIG) therapy, 14 patients recovered, 19 patients died in the hospital, and other 12 cases give up treatment because of exacerbation of the disease. Conclusion　There are various underlying diseases and clinical manifestations for HPS. HPS is always extremely dangerous situation with high mortality. Underlying diseases treatments, corticosteroids, immunodepressant and IVIG may improve the prognosis of HPS.

Clinical analyses of 86 patients with Polymyalgia rheumatica

Wei LUO; Wen ZHANG; Xiao-feng ZENG; Feng-chun ZHANG

2010, 30(2):  194-197. 

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Objective To investigate the clinical characteristics of Polymyalgia rheumatica（PMR）and the difference between male and female patients. Methods The clinical features of 86 patients with PMR were retrospectively analyzed. Results The ratio of hip and shoulder girdle muscle pain in female patients are higher than in male (P＜0.05), whereas the ratio of hyperpyrexia, arthralgia, weight loss, average level of CRP, as well as percentage of elevated immunoglobulin and leukocyte count are all higher in male patients compared with female patients（P＜0.05）. In terms of therapy response, relapsing rate was higher in female patients than in male patients. Conclusion Partial symptoms and inflammatory signs were more predominant in male patients. However, relapsing rate was higher in female patients.

研究短文

The bradykinin B2 receptor mediated in part omapatrilat inhibition of endothelin-1-induced cardiac fibroblasts proliferation in neonatal rats

Ya-ping ZHENG; Zhi-hui CAI; Yan-feng ZHAO

2010, 30(2):  198-199. 

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Upragulation of Fractalkine and CX3CR1 protein in rabbit atherosclerosis plaque

Yong-mei NIE; Huai-qing CHEN; De-qiang ZHANG

2010, 30(2):  200-202. 

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Effects of vascular endothelial growth factor C on lymph nodes metastasis and apoptosis of lung cancer cells

Xu-feng GUO; Yong-bing CHEN; Zhong-heng XU; Zhong-hua XU; Wen-tao YANG; Yong-yue QIAN

2010, 30(2):  203-204. 

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Analysis of plasma levels and clinical observation for elderly patients with congestive heart failure in Qinghai area

Ying-lian LI; Wei-shan LI

2010, 30(2):  205-205. 

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短篇综述

Inducted of pluripotent stem cells from somatic cells by reprogramming with defined genes

Zhen LIN; Xiao-li SHEN

2010, 30(2):  206-208. 

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Differentiated cells can be induced to pluripotent stem cell by reprogramming with defined fators.The new method of stem cell technology marks a major breathrough.This paper reviews the establishment, identification and research progress about induced pluripotent stem cell.

Reprogramming the human skin fibroblast cell into stem cell

Bing-zheng ZHONG; Qiao-bing HUANG

2010, 30(2):  209-211. 

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In recent years, researchers have made some breakthroughs on human embryonic stem cells, in particular, on reprogramming differentiated cell (such as human skin fibroblast) into human embryonic stem cell. Induced pluripotent stem cells could be generated from human skin fibroblasts by inducting into four transcription factors known as OCT3/4, SOX2, c-Myc and KLF4. This approach will also enable us to better understand the pathogenesis of diseases from the perspective of genetic component and is also promising in the treatment of related diseases. This article introduces the latest advancement of those researches.

Research progresses on K-RAS mutation of non-small cell lung cancer

Xian-hua FU; Jian-jin HUANG

2010, 30(2):  212-214. 

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Mutated K-RAS can been observed among 15-30% NSCLC patients,the mutations of K-RAS and EGFR are mutually exclusive,K-RAS mutations have poor sensitivity to EGFR-TKIs,The response of C-225 is not restricted to mutated K-RAS , NSCLC patients with K-RAS mutations are associated with unfavorable prognosis.A number of different approaches such as Ftase inhibitors,RAF inhibitors,MEK inhibitors aimed at abrogating K-RAS activity to improve clinical response have been explored in clinical trials.

Progress in the study of biological characteristics of mesenchymal stem cells derived from human umbilical cord

Cun-gang FAN; Jing-ru ZHOU; Qing-jun ZHANG

2010, 30(2):  215-218. 

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Recent research indictates that human umbilical cord is rich in mesenchymal stem cells. These cells have the potencies of expressing many surface markers of mesenchymal stem cell, differentiating into a variety of cells of three germ layers, synthesizing and secreting a set of trophic factors and other cytokines, and supporting the expansion and function of other cells hematopoietic stem cells and other cells. In addition, immunogenicity of these cells is realatively low.

医学教育

Role of humanistic caring in the management of doctor-patient relationship

Qi-mei SHE; Shan-shan LIN; Ya-ran DONG; Guang-yan TIAN; Feng-lan ZHAI; Xia-lian WANG

2010, 30(2):  219-220. 

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A harmonious relationship between doctors and patients is an important aspect of the socialist harmonious society. The key of harmonious relationship is to perform effective humanistic caring for patients, to thoroughly change the service model, to perfect the attitude of service; the patients should seek scientific medical help and adopt a rational attitude towards possible medical accidents. Thus the kind of concordant ambience will come into being in medical treatment.