Basic & Clinical Medicine ›› 2010, Vol. 30 ›› Issue (2): 175-178.
• 研究论文 • Previous Articles Next Articles
Qian-chuan HUANG, Jun-hao CAO, Meng JI
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Abstract: Objective To investigate the pathological significance of DEK-AP-2α interaction in HER2 overexpression and breast tumorigenesis. Methods The protein level of DEK,AP-2α and HER-2 in breast tumor tissues was detected by Western blot. The interaction of DEK and AP-2α in MDA-MB-453 mammary cancer cells was detected by immuno-coprecipitation. Furthermore the impact of DEK and AP-2α on HER2 expression was investigated by siRNA in MDA-MB-453 mammary cancer cells followed by semi-quantitive RT-PCR and Western blot. Resluts A correlation between DEK, AP-2α and HER2 levels in breast cancer tissues were observed. The interaction between DEK and AP-2α in MDA-MB-453 cells was verified by co-immunoprecipitation assay. Depletion of DEK and AP-2α in MDA-MB-453 cell by siRNA cooperatively repressed HER2 expression at both the mRNA and protein levels. Conclution This study provides oncogene DEK cooperates with AP-2α transcriptional factor to promote HER2 overexpression in breast cancer.
Key words: DEK, AP-2α, HER2, breast cancer
Qian-chuan HUANG; Jun-hao CAO; Meng JI. Oncogene DEK cooperates with AP-2α transcriptional factor to promote HER2 overexpression in breast cancer[J]. Basic & Clinical Medicine, 2010, 30(2): 175-178.
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http://journal11.magtechjournal.com/Jwk_jcyxylc/EN/Y2010/V30/I2/175