Abstract: Objective Investigated the antitumor effects of IL-18 gene transfected T cells on pancreatic cancer SW-1990. Methods: Construction of IL-18 contained recombinant lentivirus using PCR method and packaged at HEK293T cell, then transfected the T cells and evaluated the antitumor effects when cocultured with SW-1990. Results the IL-18 gene contained recombinant lentivirus was successfully constructed and packaged, and could transfected the T cells, the LDH secretion and IL-2 and IFN-γ content were all increased significantly (P < 0.01) when cocultured with SW-1990 cell. Conclusions T cells transfected with IL-18 possessed more potent antitumor effects to pancreatic cancer SW-1990 as compared to the regular T cells.

Key words: pancreatic cancer, IL-18, T cell