IL-6可增强猪卵巢颗粒细胞雄激素受体的表达

基础医学与临床 ›› 2010, Vol. 30 ›› Issue (3): 303-305.

IL-6可增强猪卵巢颗粒细胞雄激素受体的表达

洪岭滕晓明李昆明黄文强张燕张博童晓文

同济大学附属第一妇婴保健院

收稿日期:2009-05-31 修回日期:2009-10-26 出版日期:2010-03-05 发布日期:2011-05-04

IL-6 can upregulate porcine ovarian granulosa cells androgen receptor expression

Ling HONG, Xiao-ming TENG, Kun-ming LI, Wen-qiang HUANG, Yan ZHANG, Bo ZHANG, Xiao-wen TONG

Shanghai First Maternity and Infant Hospital,Tongji University

Received:2009-05-31 Revised:2009-10-26 Online:2010-03-05 Published:2011-05-04

摘要/Abstract

摘要： 目的研究白细胞介素6（IL-6）对卵泡颗粒细胞雄激素受体（AR）表达的影响，探讨IL-6在多囊卵巢综合征（PCOS）发病机制中的作用。方法在体外培养的猪卵泡颗粒细胞培养液中分别加入10、100和1000ng/L浓度的IL-6，采用实时荧光定量PCR和Western blot法观察IL-6对卵巢颗粒细胞 AR mRNA和蛋白的表达。结果 IL-6可上调卵巢颗粒细胞AR-mRNA和蛋白的表达。结论 IL-6增强雄激素的活性，可能参与了慢性亚临床炎症引发的PCOS。

关键词: IL-6, PCOS, 睾酮, 雄激素受体

Abstract: Objective To study the effect of IL-6 on the expression of androgen receptor (AR) in porcine ovarian granulosa cells(GC) and to explore the role of interleukin 6 (IL-6) in the polycystic ovary syndrome pathogenesis. Methods Different concentrations of IL-6（10、100 and 1000ng/L） were treated with GC. Expression of androgen receptor (AR) mRNA and protein in GC were detected by Real-time Quantitative RT-PCR and Western blot. Results The mRNA and protein expressions of AR in GC were upregulated in groups with 100 and 1000ng/L IL-6 at 72h. Conclusion IL-6 can improve androgen activity. This process may explain the function of chronic subclinical inflammation in PCOS pathogenesis.

Key words: interleukin 6, PCOS, testosterone, androgen receptor

洪岭滕晓明李昆明黄文强张燕张博童晓文. IL-6可增强猪卵巢颗粒细胞雄激素受体的表达[J]. 基础医学与临床, 2010, 30(3): 303-305.

Ling HONG; Xiao-ming TENG; Kun-ming LI; Wen-qiang HUANG; Yan ZHANG; Bo ZHANG; Xiao-wen TONG. IL-6 can upregulate porcine ovarian granulosa cells androgen receptor expression[J]. Basic & Clinical Medicine, 2010, 30(3): 303-305.

[1]	徐志琴, 谭晓. Asprosin在生殖细胞发育中作用的研究进展[J]. 基础医学与临床, 2024, 44(4): 558-561.
[2]	秦玉春, 邝向东, 蔡林再, 谢景臣, 陈山. 双氢青蒿素减轻慢性阻塞性肺疾病(COPD)模型大鼠肺损伤[J]. 基础医学与临床, 2023, 43(3): 427-432.
[3]	石丹丹, 蒋素芳, 王茜, 赵鹏博, 刘雅. IL-6反式信号传导通路抑制剂sgp130Fc抑制脓毒症小鼠炎性反应及调节免疫细胞[J]. 基础医学与临床, 2022, 42(12): 1895-1899.
[4]	邹威凤, 王晓茜, 盛青, 祝涛, 周晓婷. Wnt-5a/JNK信号通路参与PM2.5诱导的人支气管上皮细胞系16HBE分泌IL-6和TNF-α[J]. 基础医学与临床, 2021, 41(8): 1121-1126.
[5]	竹梅侯倩伶狄贯腾冯敏王莎莉. 微波硫灯、热辐射灯、日光灯和LED灯对雄性抑郁大鼠生殖功能的影响[J]. 基础医学与临床, 2017, 37(6): 821-827.
[6]	周智恩李汉忠严维刚. 雄激素受体蛋白表达及其基因获得/缺失与前列腺癌预后的相关性[J]. 基础医学与临床, 2010, 30(8): 857-861.
[7]	王庭槐许研刘海梅崔雨红徐进文蒋萍付晓东. 睾酮诱导大鼠心肌细胞肥大反应并上调ERK1/2蛋白表达[J]. 基础医学与临床, 2010, 30(5): 449-453.