关键词: 宫颈癌, HPV18 E6 siRNA, 基因表达, 细胞增殖活性, 化疗敏感性

Abstract: Objective To elucidates the effects of HPV18 E6 siRNA which targets human papillomavirus (HPV) 18 E6 gene on the proliferative activity of HeLa cells and chemotherapy sensitivity. Methods HPV18 E6 expressions of HeLa cells were inhibited by siRNA interference, the changes of P53 and P21 proteins expression level were measured by Western blot. MTT assay was used to detected proliferative activity and sensitivity to paclitaxel liposome in HeLa cells. Results After inhibition of E6 expression, P53 and P21 proteins level increased and the growth of HeLa cells decreased（P<0.01）. The inhibition rate of HeLa was markedly increased after transfection of HPV18 E6 siRNA and paclitaxel liposome. Conclusion HPV18 E6 siRNA can effectively silence gene expression of E6 and inhibit cell proliferation in HeLa cells. Cervical cancer HeLa cells are more sensitive to combine HPV18 E6 siRNA with paclitaxel liposome than that of control groups.

Key words: Cervical cancer, HPV 18 E6 siRNA, Gene expression, Cell Proliferation Activity, Chemotherapy Sensitivity