金黄色葡萄球菌分泌趋化抑制蛋白与C5aR,C5L2的相互作用

基础医学与临床 ›› 2010, Vol. 30 ›› Issue (5): 496-499.

金黄色葡萄球菌分泌趋化抑制蛋白与C5aR,C5L2的相互作用

刘振嘉杜冠华宫丽丽高金明

中国医学科学院北京协和医学院北京协和医院中国医学科学院北京协和医学院药物研究所中国医学科学院北京协和医学院北京协和医院呼吸科

收稿日期:2009-11-05 修回日期:2010-02-01 出版日期:2010-05-05 发布日期:2010-05-05
通讯作者: 高金明

The interaction between C5aR,C5L2 and chemotaxis inhibitory protein secreted by Staphylococcus aureus

Zhen-jia LIU, Guan-hua DU, Li-li GONG, Jin-ming GAO

PUMC Hospital, CAMS & PUMC Institute of Materia Medical, CAMS & PUMC Department of Respiratory Diseases, PUMC Hospital, CAMS & PUMC

Received:2009-11-05 Revised:2010-02-01 Online:2010-05-05 Published:2010-05-05
Contact: Jin-ming GAO

摘要/Abstract

摘要： 目的探讨金黄色葡萄球菌分泌的趋化抑制蛋白（CHIPs）与G蛋白偶联受体(GPCR) C5a受体（C5aR）或促酰化蛋白受体(C5L2))的相互作用。方法将纯化的CHIPs蛋白分别与表达C5aR, C5L2, CXC 趋化因子受体(CXCR3)的人肾胚胎上皮细胞293T分别孵育，用Western blot检测结合情况。结果 CHIPs蛋白与C5aR相结合，与C5L2和CXCR3无明显结合。结论 C5aR是CHIPs蛋白的受体，提示C5L2与C5aR在和CHIPs的结合作用上存在明显差异。

关键词: CHIPs, C5aR, C5L2, 结构关系

Abstract: Objective To investigate the physical association between G protein-coupled receptors (GPCR) including C5a receptor (C5aR), C5a Like Receptor 2 (C5L2) and chemotaxis inhibitory protein (CHIPs) secreted by Staphylococcus aureus. Methods The purified CHIPs was incubated with HEK 293T cells overexpressing C5aR, C5L2 and C-X-C chemokine receptor type 3 (CXCR3). Binding signal was detected by Western-blotting. Results HEK 293T cells overexpressing C5aR can efficiently bind to CHIPs. No apparent association between CHIPs and C5L2 or CXCR3 was detected. Conclusion C5aR not C5L2 is a membrane receptor for binding CHIPs, It suggesting a difference between C5aR and C5L2 in association with binding CHIPs.

Key words: CHIPs, C5aR, C5L2, the relationship of the structures

中图分类号:

刘振嘉杜冠华宫丽丽高金明. 金黄色葡萄球菌分泌趋化抑制蛋白与C5aR,C5L2的相互作用[J]. 基础医学与临床, 2010, 30(5): 496-499.

Zhen-jia LIU; Guan-hua DU; Li-li GONG; Jin-ming GAO. The interaction between C5aR,C5L2 and chemotaxis inhibitory protein secreted by Staphylococcus aureus[J]. Basic & Clinical Medicine, 2010, 30(5): 496-499.