关键词: 弱精症, 精子, HES1, hsa-miR-487a, hsa-miR-193b

Abstract: Objective: To analyze the expression and regulation of HES1 in sperm with low motility. Methods: Thirty semen samples from asthenospermia patients and 20 semen samples from healthy and fertile adult men were collected, and total RNAs were extracted to produce cDNAs probes. Hybridization with Phalanx OneArrayTM contained 30 968 probes was carried out after the labeled cDNAs were purified by PCR Product Purification Kit. Realtime RT-PCR was used to analyze the expression of hsa-miR-487a and hsa-miR-193b; the expression of the target genes of hsa-miR-487a and hsa-miR-193b were searched from gene-expression profiles in asthenospermia patients' sperm. Results: The expression level of HES1 in low motility sperm was up-regulated. The expression level of hsa-miR-193b in low motility sperm was 2.19 fold of that in high motility sperm, hsa-miR-487a was 0.43 fold. Conclusion: The expression level of HES1 in low motility sperm was up-regulated. Hsa-miR-487a and hsa-miR-193b might affect on the expression of HES1 and regulat sperm motility.

Key words: Asthenospermia, sperm, HES1, hsa-miR-487a hsa-miR-193b