大鼠睾丸组织发育过程中Slitrk6的表达

doi:10.16352/j.issn.1001-6325.2025.07.0912

基础医学与临床 ›› 2025, Vol. 45 ›› Issue (7): 912-917.doi: 10.16352/j.issn.1001-6325.2025.07.0912

大鼠睾丸组织发育过程中Slitrk6的表达

刘珊珊^1#, 白杰^1#, 王雪颖¹, 高林¹, 张岩¹, 刘陶迪^1*, 张信来^2*

1.内蒙古医科大学基础医学院医学神经生物学实验室,内蒙古呼和浩特 010110;
2.内蒙古医科大学附属医院普外科,内蒙古呼和浩特 010110

收稿日期:2025-02-13 修回日期:2025-04-23 出版日期:2025-07-05 发布日期:2025-06-24
通讯作者: ^*taodiliu@163.com;sdxinlaizhang@163.com
作者简介:^#对本文有相同贡献
基金资助:
内蒙古自治区自然科学基金(2022LHMS03002);内蒙古医科大学重点项目(YKD2022ZD012);内蒙古医科大学实验室开放基金指南性项目(2023ZN19)

Expression of Slitrk6 during testicular tissue development in rats

LIU Shanshan^1#, BAI Jie^1#, WANG Xueying¹, GAO Lin¹, ZHANG Yan¹, LIU Taodi^1*, ZHANG Xinlai^2*

1. Laboratory of Medical Neurobiology, School of Basic Medicine, Inner Mongolia Medical University, Hohhot 010110;
2. Department of General Surgery, Affiliated Hospital of Inner Mongolia Medical University; Hohhot 010110, China

Received:2025-02-13 Revised:2025-04-23 Online:2025-07-05 Published:2025-06-24
Contact: ^*taodiliu@163.com;sdxinlaizhang@163.com

摘要/Abstract

摘要： 目的探讨Slitrk6在大鼠精子发生中转录与翻译水平的表达情况。方法摘取2~65日龄大鼠双侧睾丸,RT-qPCR、Western blot分别检测Slitrk6在大鼠睾丸组织中mRNA转录水平及翻译水平相关表达量的变化(每日龄均做3次重复实验)。结果 Slitrk6 mRNA在第4天与第10天相对表达最高。SLITRK6蛋白在大鼠睾丸发育过程中持续表达。通过PPI网络发现Slitrk6的潜在关联靶点。结论 Slitrk6有可能参与精子发生中的有丝分裂和减数分裂。Slitrk6可能是精子发生的关键基因之一,SLITRK6蛋白可能参与有丝分裂和圆形精子变态发育过程。这些结果为后续男性不育的相关研究奠定了一些基础。

关键词: 男性不育, 睾丸组织, Slitrk6, 精子发生

Abstract: Objective To investigate the transcriptional and translational expression of Slitrk6 in rat spermatogenesis. Methods The mRNA transcription level and translation level of Slitrk6 were detected by RT-qPCR and Western blot, respectively in testis of rats aged 2-65 days (repeat three times at each time point). Results The mRNA expression of Slitrk6 reached the highest on day 4 and day 10. SLITRK6 protein was continuously expressed during testis development in rats. Slitrk6 was found in human testis through PPI network. Conclusions Slitrk6 is potentially involved in mitosis and meiosis in spermatogenesis; The Slitrk6 may be one of the key genes in spermatogenesis. SLITRK6 protein is involved in mitosis and the transformation of round spermatids.The results lay a foundation for subsequent research on male infertility.

Key words: male infertility, testicular tissue, Slitrk6, spermogenesis

中图分类号:

Q492

刘珊珊, 白杰, 王雪颖, 高林, 张岩, 刘陶迪, 张信来. 大鼠睾丸组织发育过程中Slitrk6的表达[J]. 基础医学与临床, 2025, 45(7): 912-917.

LIU Shanshan, BAI Jie, WANG Xueying, GAO Lin, ZHANG Yan, LIU Taodi, ZHANG Xinlai. Expression of Slitrk6 during testicular tissue development in rats[J]. Basic & Clinical Medicine, 2025, 45(7): 912-917.

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大鼠睾丸组织发育过程中Slitrk6的表达

Expression of Slitrk6 during testicular tissue development in rats

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