睾丸受体4蛋白和CD36蛋白在不同时期气管支气管结核黏膜中的表达

doi:10.16352/j.issn.1001-6325.2026.03.0411

基础医学与临床 ›› 2026, Vol. 46 ›› Issue (3): 411-416.doi: 10.16352/j.issn.1001-6325.2026.03.0411

睾丸受体4蛋白和CD36蛋白在不同时期气管支气管结核黏膜中的表达

罗林紫, 罗莉, 周磊, 卢志斌, 丁衍, 肖阳宝^*

湖南省结核病防治所(湖南省胸科医院) 内镜中心,湖南长沙 410013

收稿日期:2025-06-19 修回日期:2025-09-24 出版日期:2026-03-05 发布日期:2026-02-25
通讯作者: ^*xybsnow@163.com
基金资助:
湖南省卫生健康委科研计划(202203084170);湖南省科学技术厅自然科学基金(2025JJ80680)

Expressions of testicular receptor 4 protein and cluster of differentiation 36 (CD36) in the mucosa of tracheobronchial tuberculosis at different stages

LUO Linzi, LUO Li, ZHOU Lei, LU Zhibin, DING Yan, XIAO Yangbao^*

Endoscopy Center, Hunan Institute for Tuberculosis Control(Hunan Chest Hospital), Changsha 410013, China

Received:2025-06-19 Revised:2025-09-24 Online:2026-03-05 Published:2026-02-25
Contact: ^*xybsnow@163.com

摘要/Abstract

摘要： 目的检测睾丸受体4(TR4)蛋白和分化簇36(CD36)蛋白在活动期、稳定期气管支气管结核(TBTB)病变支气管黏膜中的表达,探讨其是否参与了TBTB黏膜病变的形成。方法收集TBTB患者的病变黏膜,分为活动期、稳定期、正常对照组(正常支气管黏膜),用免疫组织化学、蛋白质印记法检测TR4及CD36的表达、用流式细胞测量术检测巨噬细胞及泡沫巨噬细胞百分数,并对上述数据进行统计学分析。结果 TR4的表达水平为:活动期＞稳定期及对照组(P＜0.01)。CD36的表达水平为:活动期＞对照组＞稳定期(P＜0.05)。巨噬细胞百分数及泡沫巨噬细胞百分数:活动期＞稳定期及对照组(P＜0.05)。结论 TR4及CD36可能通过促进巨噬细胞向泡沫巨噬细胞转变,参与了活动期TBTB黏膜病变的形成。

关键词: 气管支气管结核, 睾丸受体4, 分化簇36, 巨噬细胞, 泡沫巨噬细胞

Abstract: Objective To clarify whether testicular receptor 4(TR4) protein and cluster of differentiation 36(CD36) protein are involved in the formation of tracheobronchial mucosal lesions of tracheobronchial tuberculosis (TBTB). Methods Tissues of tracheobronchial mucosal lesions of TBTB patients were collected and divided into 2 groups: active stage group and inactive stage group. The normal bronchial mucosa tissues were collected as control group. The expressions of TR4 and CD36 in 3 groups were detected by immune histochemistry and Western blot. The percentages of macrophage and foamy macrophage was detected by flow cytometry. Results The expression level of TR4 was as follows: active stage ＞ inactive stage and control group(P＜0.01). The expression level of CD36 was as follows: active stage ＞ control group ＞ inactive stage(P＜0.05). The percentages of macrophage and foamy macrophage were as follows: active stage ＞ inactive stage and control group(P＜0.05). Conclusions TR4 and CD36 seemed to play some roles in the formation of tracheobronchial mucosal lesions in active TBTB trachea the formation of foamy macrophage.

Key words: tracheobronchial tuberculosis, testicular receptor 4, cluster of differentiation 36, macrophage, foamy macrophage

中图分类号:

R523

罗林紫, 罗莉, 周磊, 卢志斌, 丁衍, 肖阳宝. 睾丸受体4蛋白和CD36蛋白在不同时期气管支气管结核黏膜中的表达[J]. 基础医学与临床, 2026, 46(3): 411-416.

LUO Linzi, LUO Li, ZHOU Lei, LU Zhibin, DING Yan, XIAO Yangbao. Expressions of testicular receptor 4 protein and cluster of differentiation 36 (CD36) in the mucosa of tracheobronchial tuberculosis at different stages[J]. Basic & Clinical Medicine, 2026, 46(3): 411-416.

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睾丸受体4蛋白和CD36蛋白在不同时期气管支气管结核黏膜中的表达

Expressions of testicular receptor 4 protein and cluster of differentiation 36 (CD36) in the mucosa of tracheobronchial tuberculosis at different stages

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