β-榄香酮酸对结肠癌细胞系增殖和侵袭的抑制作用

doi:10.16352/j.issn.1001-6325.2025.06.0748

基础医学与临床 ›› 2025, Vol. 45 ›› Issue (6): 748-754.doi: 10.16352/j.issn.1001-6325.2025.06.0748

β-榄香酮酸对结肠癌细胞系增殖和侵袭的抑制作用

孙婷¹, 邹腾¹, 杨轶嵩¹, 刘双萍¹, 任欣^1*, 王丹^2*

1.大连大学医学院检验系,辽宁大连 116622;
2.保定市第一中心医院检验科,河北保定 071000

收稿日期:2024-11-11 修回日期:2025-02-26 出版日期:2025-06-05 发布日期:2025-05-26
通讯作者: ^*renxin@dlu.edu.cn; 1872458731@qq.com
基金资助:
辽宁省创新人才支持计划(辽教函2020389)

Inhibitory effect of β-elemonic acid on proliferation and invasion of colon cancer cell lines

SUN Ting¹, ZOU Teng¹, YANG Yisong¹, LIU Shuangping¹, REN Xin^1*, WANG Dan^2*

1. Department of Laboratory Medicine, Medical College, Dalian University, Dalian 116622;
2. Clinical Laboratory, Baoding No.1 Central Hospital, Baoding 071000, China

Received:2024-11-11 Revised:2025-02-26 Online:2025-06-05 Published:2025-05-26

摘要/Abstract

摘要： 目的探讨β-榄香酮酸(β-EA)对结肠癌细胞增殖和侵袭的抑制作用及其是否通过PI3K/AKT/mTOR信号通路实现。方法使用MTT比色法和集落形成实验验证β-榄香酮酸对结肠癌细胞增殖的影响;Transwell小室侵袭实验检测β-榄香酮酸对细胞侵袭能力的影响;Western blot检测处理后细胞中PI3K/AKT/mTOR通路蛋白变化。结果 MTT实验表明β-榄香酮酸有效抑制结肠癌细胞系HCT8和HCT116的增殖,呈剂量依赖性。集落形成实验显示其对细胞增殖有抑制作用。Transwell实验显示β-榄香酮酸抑制细胞侵袭能力。Western blot显示β-榄香酮酸处理后,凋亡相关蛋cleaved-caspase 3、cleaved-caspase 9、Bax表达增加,Bcl-2表达减少;侵袭相关蛋白vimentin、Snail、MMP2、MMP9表达下调。同时,β-榄香酮酸减少p-PI3K、p-Akt、p-mTOR表达,加入PI3K抑制剂LY294002后,下调作用更显著。结论 β-榄香酮酸可能通过PI3K/AKT/mTOR信号通路抑制结肠癌细胞系HCT8和HCT116增殖及侵袭能力,可能成为治疗结肠癌的新型药物。

关键词: β-榄香酮酸, 结肠癌, 凋亡, MMP2, PI3K/Akt/mTOR

Abstract: Objective To investigate the inhibitory effect of β-elemonic acid(β-EA)on the proliferation and invasion of colon cancer cells and the underlying mechanisms through PI3K/AKT/mTOR signaling pathway. Methods The effects of β-EA on colon cancer cell proliferation were evaluated using the MTT assay and colony formation assay. Transwell invasion assay were used to assess the impact of β-EA on invasion. Western blot analysis was conducted to detect changes in PI3K/AKT/mTOR pathway proteins after treatment. Results MTT assay showed that β-EA effectively inhibited the proliferation of colon cancer HCT8 and HCT116 cells in a dose-dependent manner. The colony formation assay confirmed its inhibitory effect on cell proliferation. Transwell invasion assays demonstrated that β-elemonic acid reduced the invasion abilities of the cells. Western blot analysis revealed increased expression of apoptosis-related proteins cleaved-caspase 3, cleaved-caspase 9, and Bax, while Bcl-2 expression was decreased. Invasion-related proteins vimentin, snail, MMP2, and MMP9 were downregulated after treatment. Additionally, β-EA reduced the levels of p-PI3K, p-Akt, and p-mTOR, and these reductions were more pronounced after the addition of the PI3K inhibitor LY294002. Conclusions β-EA may inhibit proliferation and invasion in colon cancer cell lines HCT8 and HCT116 through PI3K/AKT/mTOR signaling pathway, and potentially be transformed as a novel therapeutic agent for colon cancer.

Key words: β-elemonic acid, colon cancer, apoptosis, MMP2, PI3K/Akt/mTOR pathway

中图分类号:

R285.5

孙婷, 邹腾, 杨轶嵩, 刘双萍, 任欣, 王丹. β-榄香酮酸对结肠癌细胞系增殖和侵袭的抑制作用[J]. 基础医学与临床, 2025, 45(6): 748-754.

SUN Ting, ZOU Teng, YANG Yisong, LIU Shuangping, REN Xin, WANG Dan. Inhibitory effect of β-elemonic acid on proliferation and invasion of colon cancer cell lines[J]. Basic & Clinical Medicine, 2025, 45(6): 748-754.

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β-榄香酮酸对结肠癌细胞系增殖和侵袭的抑制作用

Inhibitory effect of β-elemonic acid on proliferation and invasion of colon cancer cell lines

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