氯喹对人宫颈癌细胞系HeLa增殖、凋亡及自噬的影响

doi:10.16352/j.issn.1001-6325.2023.10.1512

基础医学与临床 ›› 2023, Vol. 43 ›› Issue (10): 1512-1517.doi: 10.16352/j.issn.1001-6325.2023.10.1512

氯喹对人宫颈癌细胞系HeLa增殖、凋亡及自噬的影响

高朋¹, 徐丹丹², 张斌³, 刘训涛³, 舒丽莎^4*

1.河北北方学院研究生院,河北张家口 075000;
河北北方学院附属第一医院 2.中心实验室;3.检验科;
4.妇科,河北张家口 075000

收稿日期:2022-10-26 修回日期:2023-04-26 出版日期:2023-10-05 发布日期:2023-10-05
通讯作者: *Lisha.shu@163.com
基金资助:
河北省政府资助临床医学优秀人才项目(361009)

Effects of chloroquine on proliferation, apoptosis and autophagy of human cervical cancer cell line HeLa

GAO Peng¹, XU Dandan², ZHANG Bin³, LIU Xuntao³, SHU Lisha^4*

1. Graduate School, Hebei North University,Zhangjikou 075000;
2. Central Laboratory;
3. Department of Clinical Laboratory;
4. Department of Gynecology, the First Affiliated Hospital of Hebei North University, Zhangjiakou 075000,China

Received:2022-10-26 Revised:2023-04-26 Online:2023-10-05 Published:2023-10-05
Contact: *Lisha.shu@163.com

摘要/Abstract

摘要： 目的探讨氯喹(CQ)对人宫颈癌细胞系HeLa增殖、凋亡及自噬的影响,并初步探讨其作用机制。方法将HeLa细胞分为对照组(不加药物)和实验组(氯喹-1组至氯喹-5组,分别用25、50、75、100、150 μmol/L CQ处理)。细胞均培养24和48 h;CCK-8法和集落形成实验检测细胞增殖;活性氧检测试剂盒检测细胞内ROS产生;流式细胞测量术检测细胞凋亡率。蛋白免疫印迹检测自噬蛋白p62、LC3和Beclin1,凋亡蛋白Bax、Bcl-2和PARP以及PI3K/AKT/MDM2通路蛋白的表达水平。结果 CQ呈时间和浓度依赖性抑制细胞增殖(P＜0.05);CQ显著抑制细胞自噬活性,诱导胞内ROS的产生,诱导细胞凋亡(P＜0.05)。与对照组相比,CQ可明显抑制PI3K/AKT/MDM2信号通路的激活(P＜0.05)。结论氯喹可能通过PI3K/AKT/MDM2通路抑制人宫颈癌HeLa细胞的增殖,诱导细胞凋亡。

关键词: 宫颈癌, 氯喹, 增殖, 凋亡, 自噬

Abstract: Objective To investigate the effect of chloroquine (CQ) on proliferation, apoptosis and autophagy of human cervical cancer cell line HeLa and to preliminarily explore its mechanism of action. Methods HeLa cells were divided into control group and experimental groups: chloroquine-1 to chloroquine-5 groups (25,50,75,100, 150 μmol/L CQ respectively). The cells all were cultured for 24 and 48 h; CCK-8 and colony formation assays were used to detect cell proliferation; reactive oxygen species detection kit was used to detect intracellular ROS production; flow cytometry was used to detect apoptosis rate. The expression level of autophagy proteins p62, LC3 and Beclin1, apoptosis proteins Bax, Bcl-2 and PARP and PI3K/AKT/MDM2 pathway proteins was detected by protein immunoblotting. Results CQ inhibited cell proliferation in a time- and concentration-dependent manner (P＜0.01); CQ significantly inhibited cell autophagy, induced intracellular ROS production and induced apoptosis (P＜0.01). Compared with control group, CQ significantly inhibited the activation of PI3K/AKT/MDM2 signaling pathway (P＜0.05). Conclusions Chloroquine may inhibit the proliferation of HeLa cells and induce apoptosis through the PI3K/AKT/MDM2 pathway.

Key words: cervical cancer, chloroquine, proliferation, apoptosis, autophagy

中图分类号:

R737.3

高朋, 徐丹丹, 张斌, 刘训涛, 舒丽莎. 氯喹对人宫颈癌细胞系HeLa增殖、凋亡及自噬的影响[J]. 基础医学与临床, 2023, 43(10): 1512-1517.

GAO Peng, XU Dandan, ZHANG Bin, LIU Xuntao, SHU Lisha. Effects of chloroquine on proliferation, apoptosis and autophagy of human cervical cancer cell line HeLa[J]. Basic & Clinical Medicine, 2023, 43(10): 1512-1517.

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氯喹对人宫颈癌细胞系HeLa增殖、凋亡及自噬的影响

Effects of chloroquine on proliferation, apoptosis and autophagy of human cervical cancer cell line HeLa

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