血浆hsa_circ_005230在原发性肝细胞癌诊断和预后评估中的临床价值

doi:10.16352/j.issn.1001-6325.2023.09.1417

基础医学与临床 ›› 2023, Vol. 43 ›› Issue (9): 1417-1422.doi: 10.16352/j.issn.1001-6325.2023.09.1417

血浆hsa_circ_005230在原发性肝细胞癌诊断和预后评估中的临床价值

依马木买买提江·阿布拉, 帕哈尔丁·白克热, 易超, 佟庆^*

新疆医科大学第三临床医学院肝胆胰外科,新疆乌鲁木齐 830000

收稿日期:2022-04-02 修回日期:2022-11-13 出版日期:2023-09-05 发布日期:2023-09-01
通讯作者: ^*tongqing2001@126.com
基金资助:
新疆自治区自然科学基金(2020D01C216)

Clinical value of plasma hsa_circ_005230 in the diagnosis and prognosis evaluation of primary hepatocellular carcinoma

Yimamaimaitijiang·ABRA, Pakhardin·BAIKERE, YI Chao, TONG Qing^*

Department of Hepatopancreatobiliary Surgery, the Third Clinical Medical College of Xinjiang Medical University, Urumqi 830000, China

Received:2022-04-02 Revised:2022-11-13 Online:2023-09-05 Published:2023-09-01
Contact: ^*tongqing2001@126.com

摘要/Abstract

摘要： 目的检测原发性肝细胞癌(HCC)血浆中环状RNA(circRNA) hsa_circ_005230表达水平,探讨其在HCC诊断和预后评估的临床价值。方法利用GEO数据库中的非编码RNA高通量测序数据筛选HCC(癌组织/癌旁组织)的circRNA表达情况。RT-qPCR检测60例HCC、30例乙型肝炎和30名健康人血浆中hsa_circ_005230表达水平,并分析其与HCC患者临床病理特征的关系。应用受试者工作特征(ROC)曲线评估hsa_circ_005230诊断HCC的临床价值。应用Kaplan-Meier法进行生存分析,Cox回归进行预后多因素分析。结果从GEO数据库中筛选出上调表达最明显的为hsa_circ_005230, HCC患者血浆中hsa_circ_005230表达水平显著高于乙型肝炎组和健康人组(P＜0.01);Hsa_circ_005230表达的增加与肿瘤大小(P＜0.05)和肿瘤淋巴结转移阶段密切相关(P＜0.01);ROC分析显示,血浆hsa_circ_005230鉴别HCC与健康人的曲线下面积(AUC)为0.69,敏感度为70%,特异度为82%。高hsa_circ_005230水平患者的总生存率显著低于低hsa_circ_005230水平患者。单因素分析显示,甲胎蛋白(AFP)(P＜0.01)和hsa_circ_005230(P＜0.01)为影响HCC预后的相关因素。Cox多变量分析显示hsa_circ_005230是 HCC 患者的独立预后指标。结论 Hsa_circ_005230可能作为诊断HCC和预后评估的新的生物学标志物。

关键词: 肝细胞癌, 环状RNA, hsa_circ_005230, 诊断, 预后

Abstract: Objective To detect the expression level of circular RNA (circRNA) hsa_circ_005230 in the plasma of primary hepatocellular carcinoma (HCC), and to explore its clinical value in the diagnosis and prognosis of HCC. Methods The non-coding RNA high-throughput sequencing data in the GEO database was used to screen the circRNA expression in HCC (cancer tissue/paracancerous tissue). RT-qPCR was used to detect hsa_circ_005230 in the plasma of 60 HCC patients, 30 hepatitis B patients and 30 healthy people. Expression levels and their relationship with clinicopathological characteristics of HCC patients were evaluated. The receiver operating characteristic(ROC) curve to evaluate the clinical value of hsa_circ_005230 in the diagnosis of HCC. Survival analysis was performed by Kaplan-Meier method, and multivariate prognostic analysis was performed by Cox regression. Results The most significant up-regulated expression was found in hsa_circ_005230 from the GEO database. The expression level of hsa_circ_005230 in plasma of HCC patients was significantly higher than that of hepatitis B group and healthy control group (P＜0.01); The increase of hsa_circ_005230 expression was related to tumor size (P＜0.05) and to the stage of tumor lymph node metastasis(P＜0.01); ROC analysis showed that the area under the ROC curve (AUC) of plasma hsa_circ_005230 in differentiating HCC from healthy people was 0.69, the sensitivity was 70%,and the specificity was 82 %.The overall survival rate of patients with high hsa_circ_005230 level was significantly lower than that of patients with low hsa_circ_005230 level. Univariate analysis showed that alpa-fetoprotein(AFP)(P＜0.01) and hsa_circ_005230 (P＜0.01) were related factors affecting the prognosis of HCC. Cox multivariate analysis revealed that hsa_circ_005230 was an independent prognostic indicator in HCC patients. Conclusions The hsa_circ_005230 may be used as a new biological marker for the diagnosis and prognostic evaluation of HCC.

Key words: hepatocellular carcinoma, circular RNA, hsa_circ_005230, diagnosis, prognosis

中图分类号:

R622
R73

依马木买买提江·阿布拉, 帕哈尔丁·白克热, 易超, 佟庆. 血浆hsa_circ_005230在原发性肝细胞癌诊断和预后评估中的临床价值[J]. 基础医学与临床, 2023, 43(9): 1417-1422.

Yimamaimaitijiang·ABRA, Pakhardin·BAIKERE, YI Chao, TONG Qing. Clinical value of plasma hsa_circ_005230 in the diagnosis and prognosis evaluation of primary hepatocellular carcinoma[J]. Basic & Clinical Medicine, 2023, 43(9): 1417-1422.

参考文献 15

[1]	Yang L, Song C, Chen Y, et al. Circular RNA circ_0103552 forecasts dismal prognosis and promotes breast cancer cell proliferation and invasion by sponging miR-1236[J]. J Cell Biochem, 2019, 120:15553-15560.
[2]	Zhao M, Dong G, Meng Q, et al. Circ_OMER1 enhances the inhibition of miR22 on CXCL6 to regulate the growth and aggressiveness of hepatocellular carcinoma cells[J]. J Cell Biochem, 2020, 121:4440-4449.
[3]	Afrin R, Arumugam S, Rahman A, et al. Curcumin ameliorates liver damage and progression of NASH in NASH-HCC mouse model possibly by modulating HMGB1-NF-κB translocation[J]. Int Immunopharmacol, 2017, 44:174-182.
[4]	Zhang HD, Jiang LH, Sun DW, et al. CircRNA: a novel type of biomarker for cancer[J]. Breast Cancer, 2018, 25:1-7.
[5]	赵钰珊,冀梦蝶,董昱诚,等.肝细胞癌中EZH2基因相关的表观遗传调控机制[J].基础医学与临床,2022,42:864-870.
[6]	Yao T, Chen Q, Fu L, et al. Circular RNAs: biogenesis, properties, roles, and their relationships with liver diseases[J]. Hepatol Res, 2017, 47:497-504.
[7]	Meng S, Zhou H, Feng Z, et al. CircRNA: functions and properties of a novel potential biomarker for cancer[J]. Mol Cancer, 2017, 16:94-101.
[8]	黄海良,丁伟伟,李冬雪. CRNDE过表达促进人肝癌细胞系HepG2增殖和迁移[J].基础医学与临床, 2020, 40:219-223.
[9]	Qi H, Sun Y, Jiang Y, et al. Upregulation of circular RNA circ_0000502 predicts unfavorable prognosis in osteosarcoma and facilitates cell progression via sponging miR-1238[J]. J Cell Biochem, 2019, 5:11-19.
[10]	Zhang LW, Wang B, Yang JX, et al. Circ-PRMT5 stimulates migration in esophageal cancer by binding miR-203[J]. Eur Rev Med Pharmacol Sci, 2020, 24:9965-9972.
[11]	Yang L, Song C, Chen Y, et al. Circular RNA circ_0103552 forecasts dismal prognosis and promotes breast cancer cell proliferation and invasion by sponging miR-1236[J]. J Cell Biochem, 2019, 120:15553-15560.
[12]	Shen GQ, Girelli D, Li L, et al. A novel molecular diagnostic marker for familial and early-onset coronary artery disease and myocardial infarction in the LRP8 gene.[J]. Circ Cardiovasc Genet, 2014, 7:514-520.
[13]	Shaohang, Cai, Zejin, et al. Risk factors associated with liver steatosis and fibrosis in chronic hepatitis B patient with component of metabolic syndrome:[J]. United European Gastroenterol J, 2018, 6:558-566.
[14]	Frisk P, Aggefors K, Cars T, et al. Introduction of the second-generation direct-acting antivirals (DAAs) in chronic hepatitis C: a register-based study in Sweden[J]. Eur J Clin Pharmacol, 2018, 74:971-978.
[15]	Mónica G, Sandra G, Miguel HK, et al. Differential roles, crosstalk and response to the antifungal protein AfpB in the three mitogen-activated protein kinases (MAPK) pathways of the citrus postharvest pathogen penicillium digitatum.[J]. Fungal Genet Biol, 2018, 124:17-28.

血浆hsa_circ_005230在原发性肝细胞癌诊断和预后评估中的临床价值

Clinical value of plasma hsa_circ_005230 in the diagnosis and prognosis evaluation of primary hepatocellular carcinoma

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