下调BECN1抑制人白血病细胞系THP-1增殖、侵袭和迁移

doi:10.16352/j.issn.1001-6325.2023.07.1104

基础医学与临床 ›› 2023, Vol. 43 ›› Issue (7): 1104-1109.doi: 10.16352/j.issn.1001-6325.2023.07.1104

下调BECN1抑制人白血病细胞系THP-1增殖、侵袭和迁移

曾燕坤^1,*, 洪小颖², 陈清林³

1.泉州医学高等专科学校健康学院,福建泉州 362000;
2.福建医科大学医学技术与工程学院, 福建福州 350004;
3.福建医科大学附属泉州第一医院检验科,福建泉州 362000

收稿日期:2022-11-24 修回日期:2023-04-03 出版日期:2023-07-05 发布日期:2023-07-05
通讯作者: ^*amy85983917@163.com
基金资助:
泉州医学高等专科学校校级科技项目(XJK2007B)

Down-regulation of BECN1 inhibits the proliferation, invasion and migration of human leukemia cell line THP-1

ZENG Yankun^1,*, HONG Xiaoying², CHEN Qinglin³

1. Department of Health,Quanzhou Medical College,Quanzhou 362000;
2. School of Medical Technology and Engineering,Fujian Medical University,Fuzhou 350004;
3. Department of Clinical Laboratory, the Affiliated Quanzhou First Hospital of Fujian Medical University,Quanzhou 362000,China

Received:2022-11-24 Revised:2023-04-03 Online:2023-07-05 Published:2023-07-05

摘要/Abstract

摘要： 目的分析下调BECN1对人白血病细胞系THP-1增殖、侵袭和迁移的影响,初步探讨其潜在的分子机制。方法采用RNA干扰技术构建靶向BECN1表达特异性shRNA的慢病毒及阴性对照。将THP-1细胞分为对照组(control)、阴性对照组(NC)、BECN1干扰组(BECN1-shRNA)。分别采用对应慢病毒感染后,经嘌呤霉素筛选稳转的细胞株。RT-qPCR和Western blot鉴定BECN1下调效果。MTT法检测THP-1细胞增殖能力;Transwell小室法分析THP-1细胞侵袭和迁移能力。Western blot检测THP-1细胞中基质金属蛋白酶MMP 2、MMP 9蛋白表达。结果经嘌呤霉素筛选获得了表达GFP的THP-1稳转细胞株。与对照组、阴性对照组相比,BECN1-shRNA组THP-1细胞中BECN1 mRNA和蛋白表达均显著下调(P<0.05);其细胞增殖、侵袭和迁移能力均明显减弱(P<0.05);且MMP 2、MMP 9蛋白表达水平均下降(P<0.05)。结论下调BECN1可明显抑制THP-1细胞的增殖、侵袭和迁移能力,其分子机制与MMP 2、MMP 9的下调有关。

关键词: BECN1, 白血病, 增殖, 侵袭, 迁移

Abstract: Objective To analyze the effect of down-regulation of BECN1 on the proliferation,invasion and migration of human leukemia cell line THP-1,and to explore its potential molecular mechanism. Methods The lentivirus expressing shRNA targeting at BECN1 and the negative control were constructed by RNA interference. THP-1 cells were divided into control group(control),negative control group(NC) and BECN1 interference group (BECN1-shRNA). Stable cell strains were selected by puromycin after infection by lentivirus. The down-regulation effect of BECN1 was identified by RT-qPCR and Western blot. MTT and Transwell assays were used respectively to detect the proliferation,invasion and migration of THP-1 cells. The expression of MMP 2 and MMP 9 were detected by Western blot. Results THP-1 stable cell strain expressing GFP was obtained by puromycin selection. Compared with the control and negative control group, the BECN1 mRNA and protein expression in the BECN1-shRNA group were significantly decreased (P<0.05); The proliferation,invasion and migration of cells also decreased significantly(P<0.05);The expression of MMP 2 and MMP 9 decreased (P<0.05). Conclusions Down-regulation of BECN1 may inhibit the proliferation,invasion and migration of THP-1 cells,and its molecular mechanism is related to the down-regulation of MMP 2 and MMP 9.

Key words: BECN1, leukemia, proliferation, invasion, migration

中图分类号:

R733.71

曾燕坤, 洪小颖, 陈清林. 下调BECN1抑制人白血病细胞系THP-1增殖、侵袭和迁移[J]. 基础医学与临床, 2023, 43(7): 1104-1109.

ZENG Yankun, HONG Xiaoying, CHEN Qinglin. Down-regulation of BECN1 inhibits the proliferation, invasion and migration of human leukemia cell line THP-1[J]. Basic & Clinical Medicine, 2023, 43(7): 1104-1109.

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下调BECN1抑制人白血病细胞系THP-1增殖、侵袭和迁移

Down-regulation of BECN1 inhibits the proliferation, invasion and migration of human leukemia cell line THP-1

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