桔梗皂苷D增强人直肠癌耐药细胞株SW1463/Oxa对奥沙利铂的敏感性

doi:10.16352/j.issn.1001-6325.2023.04.0603

基础医学与临床 ›› 2023, Vol. 43 ›› Issue (4): 603-607.doi: 10.16352/j.issn.1001-6325.2023.04.0603

桔梗皂苷D增强人直肠癌耐药细胞株SW1463/Oxa对奥沙利铂的敏感性

闫军¹, 冯永安², 石永奎¹, 赵志浩¹, 倪怀亮¹, 杨维桢^1*

1.宝鸡市中心医院普外科,陕西宝鸡 721008;
2.西安医学院附属宝鸡医院肝胆胰脾外科,陕西宝鸡 721006

收稿日期:2022-03-16 修回日期:2022-10-25 出版日期:2023-04-05 发布日期:2023-04-03
通讯作者: ^*oicqyj_sx@163.com
基金资助:
宝鸡市卫生和计划生育局资助项目(2017-12)

Platycodon grandiflorum saponin D enhances the sensitivity of human rectal cancer cell strain SW1463/Oxa with drug resistance to oxaliplatin

YAN Jun¹, FENG Yong'an², SHI Yongkui¹, ZHAO Zhihao¹, NI Huailiang¹, YANG Weizhen^1*

1. Department of General Surgery, Baoji Central Hospital, Baoji 721008;
2. Department of Hepatobiliary Pancreatic Splenic Surgery, Baoji Hospital, Xi'an Medical College, Baoji 721006, China

Received:2022-03-16 Revised:2022-10-25 Online:2023-04-05 Published:2023-04-03
Contact: ^*oicqyj_sx@163.com

摘要/Abstract

摘要： 目的探讨桔梗皂苷D对人直肠癌耐药细胞株SW1463/奥沙利铂(Oxa)化学治疗敏感性的增强作用。方法取人直肠癌细胞系SW1463,建立稳定的耐奥沙利铂细胞株SW1463/Oxa。将构建成功的人直肠癌耐药细胞株SW1463/Oxa随机分为桔梗皂苷D组、Oxa组、桔梗皂苷D+Oxa组和对照组。MTT法检测细胞增殖曲线;RT-qPCR检测脱氧核糖核酸甲基转移酶3a(DNMT3a)、磷酸化组蛋白γ-H2AX、DNA双链修复蛋白RAD51、信号传导子和转录激活子3(STAT3)信使核糖核酸(mRNA)表达;Western blot检测细胞DNMT3a、γ-H2AX、RAD51、STAT3蛋白表达及磷酸化STAT3(p-STAT3)水平。结果成功构建人直肠癌耐药细胞株SW1463/Oxa;与对照组和Oxa组比,桔梗皂苷D组和桔梗皂苷D+Oxa组细胞增殖能力、DNMT3a、RAD51、STAT3 mRNA和蛋白表达以及p-STAT3水平降低(P＜0.05),γ-H2AX mRNA和蛋白表达升高(P＜0.05)。结论桔梗皂苷D能增强人直肠癌耐药细胞株SW1463/Oxa对Oxa的化疗敏感性,推测是通过抑制DNMT3a、RAD51、STAT3的表达,促进γ-H2AX的表达实现此作用的。

关键词: 桔梗皂苷D, 脱氧核糖核酸甲基转移酶3a, 直肠癌, 敏感性

Abstract: Objective To investigate the enhancing effect of platycodon grandiflorum saponin D on the sensitivity of human rectal cancer drug-resistant cell line SW1463 to oxaliplatin (Oxa). Methods Human rectal cancer cell line SW1463 was collected to establish a stable oxaliplatin-resistant cell strain SW1463/Oxa. The constructed cell strain SW1463/Oxa was randomly divided into platycodon grandiflorum saponin D group, Oxa group, platycodon saponin D+Oxa group and control group. The cell proliferation curve was detected by MTT. Deoxyribonucleic acid methyltransferase 3a (DNMT3a), phosphorylated histone γ-H2AX, DNA double strand repair protein RAD51 and signal transducer and activator of transcription 3(STAT3) messenger RNA (mRNA) expression were detected by RT-qPCR. DNMT3a, γ-H2AX, RAD51, STAT3 protein expression and phosphorylated STAT3(p-STAT3) level were detected by Western blot. Results Human rectal cancer drug resistant cell strain SW1463/Oxa was successfully constructed. Compared with the control group and Oxa group, the cell proliferation, DNMT3a, RAD51, STAT3 mRNA and protein expression and p-STAT3 level of platycodon saponin D group and platycodon saponin D+Oxa group decreased(P＜0.05), and the expression of γ-H2AX mRNA and protein increased(P＜0.05). Conclusions Platycodon grandiflorum saponin D may enhance the chemo-sensitivity of human rectal cancer drug-resistant cell strain SW1463/Oxa to Oxa, and the potential mechanism is inhibition of expression of DNMT3a, RAD51, STAT3 and promotion of expression of γ-H2AX.

Key words: platycodon grandiflorum saponin D, deoxyribonucleic acid methyltransferase 3a, rectal cancer, susceptibility

中图分类号:

R735.3

闫军, 冯永安, 石永奎, 赵志浩, 倪怀亮, 杨维桢. 桔梗皂苷D增强人直肠癌耐药细胞株SW1463/Oxa对奥沙利铂的敏感性[J]. 基础医学与临床, 2023, 43(4): 603-607.

YAN Jun, FENG Yong'an, SHI Yongkui, ZHAO Zhihao, NI Huailiang, YANG Weizhen. Platycodon grandiflorum saponin D enhances the sensitivity of human rectal cancer cell strain SW1463/Oxa with drug resistance to oxaliplatin[J]. Basic & Clinical Medicine, 2023, 43(4): 603-607.

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桔梗皂苷D增强人直肠癌耐药细胞株SW1463/Oxa对奥沙利铂的敏感性

Platycodon grandiflorum saponin D enhances the sensitivity of human rectal cancer cell strain SW1463/Oxa with drug resistance to oxaliplatin

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