关键词: lncRNA-ATB, NF-κB信号通路, 增殖, 胶原合成

Abstract: Objective To investigate the effects of lncRNA-ATB on the proliferation and collagen synthesis of human keloid fibroblast (KFB) and its possible molecular mechanism. Methods Human KFB and normal fibroblasts (NFB) were isolated and cultured in vitro, KFB was divided into NC group, si-NC group, si-lncRNA-ATB group, si-lncRNA-ATB+TNF-α group. RT-qPCR was used to detect the expression of lncRNA-ATB in NFB and KFB. MTT was used to detect cell activity. Flow cytometry was used to detect cell cycle. Western blot was used to detect expression of cyclinD1, collagen synthesis and NF-κB signaling pathway related protein. Results The expression of lncRNA-ATB in KFB cells was significantly increased (P＜0.05). Knock-down lncRNA-ATB expression decreased the A value of KFB cells, the expression of cyclinD1 and the percentageof S phase cells,but increased the percent- age of G₀/G₁ phase cells (P＜0.05); the expression of Col-Ⅰ and Col-Ⅲ of collagen was decreased(P＜0.05); the expression of p-p65 and p-IκBα in NF-κB signaling pathway was inhibited(P＜0.05); NF-κB activation activator TNF-α alleviated the inhibitory effect of knock-down lncRNA-ATB expression on KFB cell proliferation and collagen synthesis (P＜0.05). Conclusions Knock-down of lncRNA-ATB expression may inhibit KFB proliferation and collagen synthesis by down-regulating NF-κB signaling pathway.

Key words: lncRNA-ATB, NF-κB signaling pathway, proliferation, collagen pathway