X线对秀丽隐杆线虫DNA损伤修复基因表达的影响

基础医学与临床 ›› 2021, Vol. 41 ›› Issue (4): 479-483.

X线对秀丽隐杆线虫DNA损伤修复基因表达的影响

陈利娜^1,3, 刘志², 张超^1*

1.南方医科大学基础医学院生化与分子生物学教研室,广东广州 510515;
2.南方医院超声科,广东广州 510000;
3.湘南学院基础医学院,湖南郴州 423000

收稿日期:2020-05-12 修回日期:2020-08-12 出版日期:2021-04-05 发布日期:2021-04-05
通讯作者: ^*zhangchao1558@126.com
基金资助:
国家自然科学基金(81872557);湖南省自然科学基金(2019JJ50569)

Effects of X-ray radiation on expression of DNA damage repair related genes in C.elegans

CHEN Li-na^1,3, LIU Zhi², ZHANG Chao^1*

1. Department of Biochemistry and Molecular Biology, School of Basic Medical Science, Southern Medical University, Guangzhou 510515;
2. Department of Ultrasound, Nanfang Hospital, Guangzhou 510000;
3. Basic Medical College, Xiangnan University, Chenzhou 423000, China

Received:2020-05-12 Revised:2020-08-12 Online:2021-04-05 Published:2021-04-05
Contact: ^*zhangchao1558@126.com

摘要/Abstract

摘要： 目的研究不同高剂量X线对秀丽隐杆线虫DNA损伤修复相关基因的表达和死亡率、发育力的影响。方法分别用200、400Gy X线照射秀丽隐杆线虫后,RT-qPCR检测14个DNA损伤修复相关基因的表达及各基因间相互作用。在线虫辐射后继续培养2 d,计算秀丽隐杆线虫的存活率,以评估X线对线虫死亡率和生育力的影响。结果与对照组相比,X线使秀丽隐杆线虫多个DNA损伤修复相关基因表达异常,线虫死亡率增加(P＜0.05),繁殖力下降(P＜0.05)。结论 X线可能是通过改变秀丽隐杆线虫多个DNA损伤修复相关基因的表达,促进细胞凋亡,抑制其繁殖能力。age-1、cep-1、ced-4、rad-51和daf-2等基因对辐射敏感,是潜在的放疗靶点。

关键词: 电离辐射, 秀丽隐杆线虫, 基因表达, 凋亡, 生育力

Abstract: Objective To study the effects of different high-dose X-ray on the expression of DNA damage repair related genes, the mortality and fertility in C.elegans. Methods Wild-type C.elegans were irradiated with 200 Gray (Gy) or 400 Gy of X-ray, then the expression of 14 DNA damage-repair related genes were quantified by RT-qPCR. STRING was adopted to analyze the protein-protein interaction of the differential genes.C.elegans survival was counted to assess the effect of X-ray on nematodes mortality and fertility. Results X-ray radiation caused abnormal expression of multiple DNA damage repair related genes in C.elegans as compared to the control group. Mortality of C.elegans increased(P＜0.05)and fecundity decreased(P＜0.05)after radiation. Conclusions X-ray radiation promotes apoptosis and inhibits fertility of C.elegans by changing the expression of multiple DNA damage and oxidation damage related genes. The age-1,cep-1,ced-4,rad-51 and daf-2 are sensitive to radiation, which are potential targets for radiotherapy.

Key words: ionizing radiation, C.elegans, gene expression, apoptosis, fertility

中图分类号:

R339.5+7

陈利娜, 刘志, 张超. X线对秀丽隐杆线虫DNA损伤修复基因表达的影响[J]. 基础医学与临床, 2021, 41(4): 479-483.

CHEN Li-na, LIU Zhi, ZHANG Chao. Effects of X-ray radiation on expression of DNA damage repair related genes in C.elegans[J]. Basic & Clinical Medicine, 2021, 41(4): 479-483.

参考文献

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