关键词: [关键词] 神经干细胞, 纤维蛋白支架, 增殖, 分化, SHH

Abstract: Objective To study the effects of fibrin scaffold that can release recombinant SHH adenovirus slowly on the proliferation and differentiation of neural stem cells (NSCs) . Methods Experimental group: NSCs were planted in recombinant SHH adenovirus-scaffold group, culture plate, recombinant SHH adenovirus transfection group, fibrin scaffold group as control group(Controls). The neural stem cells were isolated, cultured and identified in vitro ; Construction of fibrin scaffold that can release recombinant SHH adenovirus slowly. The efficiency of transfection was determined by immunofluorescence and Western blotting. The cell proliferation was tested by MTT. Immunofluorescence and immunoblotting were used to detect the expression of β-tubulin III, and MBP and GFAP in NSCs after inducing in each group. Results NSCs cultured in vitro expressed nestin highly. Data shows, NSCs stably expressed SHH after transfection with recombinant SHH adenovirus; FG-SHH scaffold can promote the proliferation of NSCs, and the positive rates and protein expression.

Key words: [Key words] Neural stem cells, Fibrin glue scaffold, Proliferation, Differentiation, SHH