基础医学与临床 ›› 2019, Vol. 39 ›› Issue (7): 995-1000.

• 研究论文 • 上一篇    下一篇

恒河猴增龄过程中miR-16-5p及靶基因在外周血白细胞中的表达变化

胡婧雯1,李艳艳1,刘伟2,焦丽3,杨晶3,杨云3,和占龙1,彭小忠4,鲁帅尧1   

  1. 1. 中国医学科学院医学生物学研究所
    2. 中国医学科学院 基础医学研究所 北京协和医学院 基础学院
    3. 中国医学科学院、北京协和医学院医学生物学研究所
    4. 中国医学科学院 基础医学研究所 北京协和医学院 基础学院 医学分子生物学国家重点实验室
  • 收稿日期:2019-05-07 修回日期:2019-05-21 出版日期:2019-07-05 发布日期:2019-07-02
  • 通讯作者: 鲁帅尧 E-mail:lushuaiyao@imbcams.com.cn
  • 基金资助:
    中国医学科学院医学与健康科技创新工程重大协同创新项目,衰老及衰老相关疾病发病机制与干预研究

Expression of miR-16-5p and its target mRNA in peripheral blood leukocytes of rhesus mulatta

  • Received:2019-05-07 Revised:2019-05-21 Online:2019-07-05 Published:2019-07-02

摘要: 目的 明确衰老相关microRNA与恒河猴增龄的相关性。方法 将猴群参照人类增龄划分为幼年、成年、老年和长寿,提取外周血白细胞的总RNA;用real-time PCR检测外周血白细胞中的microRNA及VEGFA、GHR、TBP、INSR、CLOCK、IKBKB、PIK3R1、LRP2、SSTR3、IGF1R、BCL2、PPM1D、IRS1、MAPK8、ADCY5、APP、NFE2L1、BDNF和PEX5的表达;利用microRNA靶基因预测网站miRwalk、TargetSan、miRecords 、miRDB预测miR-16-5p的靶基因,并与人类衰老基因库(http://genomics.senescence.info/)对接。结果 在增龄过程中恒河猴外周血白细胞中miR-16-5p的表达量随年龄增长而增加(P<0.001);多个网站对miR-16-5p靶基因预测得到539个潜在的靶基因,与人类衰老基因组库对接后有19个基因重合;有10个基因的mRNA在恒河猴增龄过程中存在差异表达,其中, IKBKB、PIK3R1、IRS1的表达随着年龄的增加逐渐下降。结论 在自然衰老恒河猴的外周血白细胞中,miR-16-5p及其靶基因IKBKB、PIK3R1、IRS1能作为外周血衰老候选标志物。

关键词: 衰老, 恒河猴, miR-16-5p, 标志物

Abstract: Objective To clarify the correlation between microRNA and aging in rhesus mulatta. Methods The monkeys were divided into four different age groups according to human aging, which were named the infant, the adult, the old and the longevity. Real-time PCR was used to detect the expression of microRNA and VEGFA, GHR, TBP, INSR, CLOCK, IKBKB, PIK3R1, LRP2, SSTR3, IGF1R, BCL2, PPM1D, IRS1, MAPK8, ADCY5, APP, NFE2L1, BDNF, PEX5 in peripheral blood leukocytes. Using microRNA target genes forecast website miRwalk, TargetSan, miRecords, miRDB to predicte miR-16-5p target genes. Next the predicted results were docked with Human Ageing Genomic Resources (http://genomics.senescence.info/). Results The expression of miR-16-5p in peripheral blood leukocytes of aging rhesus macaques tended to increase with age (P<0.001). The predicted target genes of miR-16-5p were 539, and 19 of these were overlapped with Human Ageing Genomic Resources. It was showed that the 10 mRNA of 19 genes were detected and their expression were differences in the aging process of rhesus monkeys. The expressions of IKBKB, PIK3R1 and IRS1 were decreased gradually with aging compared with the infant group. Conclusions The miR-16-5p can be used as a biomarker of peripheral blood leukocytes in rhesus monkeys. And IKBKB, PIK3R1, IRS1 may be the target genes of miR-16-5p in the aging process of rhesus monkeys.

Key words: Aging, rhesus mulatta, miR-16-5p, Maker

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