基础医学与临床 ›› 2018, Vol. 38 ›› Issue (12): 1737-1742.

• 研究论文 • 上一篇    下一篇

miR-548p表达上调抑制卵巢癌细胞系SKOV-3的增殖和迁移

古力米热·布然江1,热孜亚?库尔班2,艾力克木?阿不都玩克3,李小文1,古丽娜?库尔班2   

  1. 1. 新疆医科大学附属肿瘤医院
    2. 新疆医科大学第三临床医学院
    3. 新疆维吾尔自治区人民医院
  • 收稿日期:2018-03-12 修回日期:2018-05-30 出版日期:2018-12-05 发布日期:2018-11-23
  • 通讯作者: 古力米热·布然江 E-mail:glmr659@163.com

Up-regulated mir-548p expression inhibits the proliferation and migration of ovarian cancer cell line SKOV-3

  • Received:2018-03-12 Revised:2018-05-30 Online:2018-12-05 Published:2018-11-23

摘要: 目的 探讨miR-548p对卵巢癌细胞系SKOV-3细胞增殖和迁移能力的影响,阐明miR-548p发挥功能的相关作用机制。方法 实时荧光定量PCR分析卵巢癌组织和癌旁组织以及卵巢癌细胞系中miR-548p的表达;用miR-548p模拟物作用于SKOV-3细胞, MTT和Transwell方法检测SKOV-3细胞增殖及迁移;Western blot检测细胞中基质金属蛋白酶、线粒体凋亡蛋白以及NF-κB。结果 相对于癌旁卵巢组织及人卵巢上皮细胞系,miR-548p在卵巢癌组织以及卵巢癌细胞系中低表达(P<0.05);增加miR-548p的表达可以抑制SKOV-3细胞的增殖及迁移,伴随相关MMP-2、MMP-9以及Bcl-2蛋白的表达减少,Bax蛋白的表达增加(P<0.05);miR-548p的表达增加可显著抑制SKOV-3细胞p-p65含量(P<0.05)。结论 miR-548p通过降低p65磷酸化水平调控卵巢癌细胞的增殖和迁移,为卵巢癌的治疗提供了一个新的靶点。

关键词: miR-548p, NF-κB, 卵巢癌细胞, 增殖, 迁移

Abstract: Objective To study the effects of miR-548p on the proliferation and migration of SKOV-3 cells in ovarian cancer cell lines in vitro, and its relevant mechanism. Methods The expression of miR-548p in ovarian cancer tissues,paracancerous tissue and ovarian cancer cell lines were analyzed by Real-time fluorescence quantitative PCR. Moreover, the effects of miR-548p on SKOV-3 cell proliferation and migration were explored by MTT and Transwell assays using miR-548p mimics in SKOV-3 cells. Western blot method was used to detect the effects of miR-548p on matrix metalloproteinases, mitochondrial apoptosis related proteins and NF-κB signaling pathway in SKOV-3 cells. Results MiR-548p was poorly expressed in ovarian tissues and ovarian cancer cell lines compared to adjacent normal ovary tissues and cell lines. The increase of miR-548p expression inhibited the proliferation and migration of SKOV-3 cells, decreased the related MMP-2, MMP-9 and Bcl-2 expression, and increased Bax expression. Conclusion MiR-548p regulates the proliferation and migration of ovarian cancer cells through reducing the phosphorylation level of p65, and provides a new target for the treatment of ovarian cancer.

Key words: miR-548p, NF-κB signaling pathways, Ovarian cancer cells, Proliferation, Migration