关键词: TRIM11, miR-24-3p, 乳腺癌, 增殖, 侵袭

Abstract: Objective To investigate the effect of TRIM11 (tripartite motif-containing protein 11) target regulating MicroRNA-24-3p (miR-24-3p) on the proliferation and invasion of breast cancer cells, and the relation between TRIM11 high expression and the prognosis of breast cancer. Methods Immunohistochemical method was used to detect the expression of TRIM11 in 31 cases of breast cancer and 31 cases of adjacent breast cancer normal tissues. The siRNA TRIM11 lentivirus and miR-24-3p lentivirus was transfected into human breast cancer MCF7 cell lines, observe the correlation expression of TRIM11 and miR-24-3p mRNA and protein by using real-time fluorescence quantitative PCR (RT-qPCR) and Western bolt, luciferase reporter experiments was used to verify that the miR-24-3p as a direct target of TRIM11, MTT and Transwell were used to investigate the cells proliferation, activity and invasion. Results The expression of TRIM11 in breast cancer tissues or MCF7 cells was significantly higher (P < 0.05), and we found TRIM11 high expression predicts poor prognosis in breast cancer patients (P < 0.05). siRNA TRIM11 significantly inhibited the expression of TRIM11 in MCF7 cells, moreover, which inhibited the MCF7 cells proliferation, viability and invasion (P < 0.05). miR-24-3p significantly reduced 3，-UTR TRIM11 luciferase activity in wild-type (P < 0.05), however, no effect was found in mutant type. The expression of miR-24-3p was decreased, miR-24-3p and TRIM11 mRNA expression was negatively correlated in MCF7 cells (P < 0.05), miR-24-3p inhibition protein and mRNA expression of TRIM11 in MCF7 cells, and inhibit MCF7 cells proliferation, viability and invasion (P<0.05). Conclusions The expression of TRIM11 is up-regulated in breast cancer and cells, promote the proliferation and invasion of breast cancer cells though regulating miR-24-3p expression, TRIM11 high expression predicts poor prognosis in breast cancer, TRIM11/ miR-24-3p axis is expected to become a new target for treatment of breast cancer.

Key words: TRIM11, miR-24-3p, breast cancer, proliferation, invasion