关键词: 白藜芦醇, HUVEC细胞, 凋亡, 线粒体跨膜电位

Abstract: Objective: To investigate the effects of resveratrol on Bcl-2/Bax expression, mitochondrial membrane potential and the NF-κB nuclear translocation of the HUVEC cells induced by A?1-42.Methods: HUVEC cells were stimulatedby A?1-42 5×101μmol/L 24 hours, 160,80,40,20μg/L resveratrol were adminstrated,cell viability was observed by CCK-8; NF-κB nuclear translocation and mitochondrial membrane potential were tested by the fluorescence microscope,expression of NF-κB/Bcl-2/Bax protein were detected by Western blot.Results: Compared with A?1-42 group, resveratrol could increase the cell viability and the mitochondrial membrane potential,inhibited the NF-κB nuclear translocation , and reduced the expression of NF-κB in nucleus,reduced the expression of Bax,increased the expression of Bcl-2 ,increased the Bcl-2/Bax ratio significantly (P<0.05). Conclusion: Resveratrol could against the injure of HUVEC cell induced by A?1-42, and the mechanism may be associated with inhibitig the NF-κB nuclear translocation ,increasing the mitochondrial membrane potential and increased the Bcl-2/Bax ratio.

Key words: resveratrol, HUVEC cell , apopotosis, mitochondrial membrane potential