关键词: Ifi204 基因, 血管外膜成纤维细胞, 凋亡, 迁移

Abstract: Objective To investigate effects of Ifi204 gene over-exprssion and silencing on apoptosis and migration of rat vascular adventitial fibroblast cell(VAF). Method Rat VAF were cultured and transfected Ifi204 gene by lentivirus particles(ifi204lv group) or empty vector lentivirus particles(blanklv group),respectively,as the over-exprssion group and blank group. Instantaneous intervention VAF by ifi204 small interfering(ifi204siRNA group) or blank small interfering RNA (Control SiRNA group) ,as ifi204siRNA group and control SiRNA group. VAF was untreated as a negative group .Cell apoptosis was detected by flow cytometry;cell migration by cell scratch and transwell;the mRNA and protein expression of p204/p53 were detected by Real-Time PCR and Western blot. Result Compared with those in the blank lv group ,controlsiRNA group ,ifi204siRNA group or Negative group, in ifi204lv group ,P204/P53 mRNA and protein expression and rate of apoptosis significantly increased (P<0.05), and cell migration speed reduced (P<0.05). Transfection of ifi204siRNA inhibit P204 /P53 mRNA and protein expression(P<0.05), improves cell viability(P<0.05), inhibits cell apoptosis(P<0.05), accelerates the speed of cell migration(P<0.05). Conclusion Effects of ifi204 gene expression on apoptosis and migration of rat vascular adventitial fibroblast may be related to activation of p53.

Key words: Ifi204 genne, vascular adventitial fibroblast, apoptosis, migration