榆栀止血颗粒减轻宫腔粘连大鼠子宫内膜纤维化

doi:10.16352/j.issn.1001-6325.2022.08.1220

基础医学与临床 ›› 2022, Vol. 42 ›› Issue (8): 1220-1224.doi: 10.16352/j.issn.1001-6325.2022.08.1220

榆栀止血颗粒减轻宫腔粘连大鼠子宫内膜纤维化

姜艳玲¹, 丁丽², 李发余¹, 解世雷¹, 张一琼^3*

1.中药制药共性技术国家重点实验室, 山东临沂 276006;
2.山东省日照市人民医院妇科, 山东日照 276826;
3.南昌大学第一附属医院妇产科, 江西南昌 330006

收稿日期:2021-04-15 修回日期:2021-10-15 出版日期:2022-08-05 发布日期:2022-08-01
通讯作者: ^*ddnm56@163.com
基金资助:
江西省卫生计生委科技计划(20185126)

Yuzhi Zhixue granules reduce endometrial fibrosis in rats with intrauterine adhesion

JIANG Yan-ling¹, DING Li², LI Fa-yu¹, JIE Shi-lei¹, ZHANG Yi-qiong^3*

1. State Key Laboratory of Generic Technology of Traditional Chinese Medicine, Linyi 276006;
2. Department of Gynecology, Rizhao People's Hospital, Rizhao 276826;
3. Department of Obstetrics and Gynecology, the First Affiliated Hospital of Nanchang University 330006, China

Received:2021-04-15 Revised:2021-10-15 Online:2022-08-05 Published:2022-08-01
Contact: ^*ddnm56@163.com

摘要/Abstract

摘要： 目的探讨榆栀止血颗粒对宫腔粘连大鼠基质细胞衍生因子-1(SDF-1)/CXC趋化因子受体4(CXCR4)轴及内膜纤维化的影响。方法将大鼠随机分成假手术组、模型组(建立宫腔粘连大鼠模型)、榆栀止血颗粒低、中、高剂量组,分别灌胃给予30、60和120 mg/kg,1次/d,连续21 d。末次灌胃24 h后,试剂盒检测血清中白介素6(IL-6),肿瘤坏死因子α(TNF-α),转化生长因子β1(TGF-β1)水平;HE染色和Masson染色检测大鼠子宫内膜腺体数量和间质纤维化程度;蛋白免疫印迹法检测SDF-1、CXCR4蛋白表达。结果与假手术组相比,模型组大鼠子宫组织结构破坏,腺体数量减少,纤维化增生,可见大量炎性细胞浸润,血清IL-6、TNF-α、TGF-β1水平显著升高(P＜0.05),子宫组织中SDF-1蛋白表达显著升高(P＜0.05)。与模型组相比,榆栀止血颗粒低、中、高剂量组的腺体数量依次增多,纤维化程度减弱,炎性细胞减少,血清中IL-6、TNF-α、TGF-β1水平显著降低(P＜0.05),子宫中SDF-1蛋白表达显著降低(P＜0.05),且均呈剂量依赖性。结论榆栀止血颗粒可能通过抑制SDF-1/CXCR4轴,修复宫腔粘连大鼠子宫结构,缓解组织纤维化,减轻炎性反应。

关键词: 榆栀止血颗粒, 宫腔粘连, 基质细胞衍生因子-1, CXC趋化因子受体4, 内膜纤维化

Abstract: Objective To investigate the effects of Yuzhi Zhixue granules on stromal cell-derived factor-1 (SDF-1)/CXC chemokine receptor 4 (CXCR4) axis and endometrial fibrosis in rats with intrauterine adhesions. Methods The rats were randomly divided into sham operation group, model group and Yuzhi Zhixue granules low, medium and high dose groups(to establish a rat model of intrauterine adhesions), and were given 30 mg/kg, 60 mg/kg and 120 mg/kg by gavage respectively, once a day for 21 consecutive days. The levels of serum interleukin-6(IL-6), tumor necrosis factor-α (TNF-α) and transforming growth factor-β1 (TGF-β1) were detected by kits; HE staining and Masson staining were used to detect the number of endometrial glands and the degree of interstitial fibrosis; the protein expressions of SDF-1 and CXCR4 were detected by Western blot. Results Compared with those in the sham operation group, the uterine tissue structure of the model group was destroyed, the number of glands decreased, fibrotic hyperplasia was found and a large number of inflammatory cells infiltration was found. The levels of serum IL-6, TNF-α and TGF-β1 were significantly higher (P＜0.05) and the expression of SDF-1 protein in uterine tissue was significantly lower (P＜0.05). Compared with those in the model group, the number of glands in the low, medium and high dose groups of Yuzhi Zhixue Granules increased in turn, the degree of fibrosis was weakened, and the number of inflammatory cells was decreased, the levels of IL-6, TNF-α and TGF-β1 in serum were significantly lower (P＜0.05). The expression of SDF-1 protein in uterus was significantly lower (P＜0.05), and all of them were dose-dependent. Conclusions Yuzhi Zhixue granules can repair the uterine structure of rats with intrauterine adhesions, alleviate tissue fibrosis and reduce inflammatory reaction by inhibition of SDF-1/CXCR4 axis.

Key words: Yuzhi Zhixue granule, intrauterine adhesion, stromal cell-derived factor-1, CXC chemokine receptor 4, endometrial fibrosis

中图分类号:

R711.7

姜艳玲, 丁丽, 李发余, 解世雷, 张一琼. 榆栀止血颗粒减轻宫腔粘连大鼠子宫内膜纤维化[J]. 基础医学与临床, 2022, 42(8): 1220-1224.

JIANG Yan-ling, DING Li, LI Fa-yu, JIE Shi-lei, ZHANG Yi-qiong. Yuzhi Zhixue granules reduce endometrial fibrosis in rats with intrauterine adhesion[J]. Basic & Clinical Medicine, 2022, 42(8): 1220-1224.

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榆栀止血颗粒减轻宫腔粘连大鼠子宫内膜纤维化

Yuzhi Zhixue granules reduce endometrial fibrosis in rats with intrauterine adhesion

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