电刺激促进大鼠脊髓钝挫伤后运动功能恢复

基础医学与临床 ›› 2020, Vol. 40 ›› Issue (3): 363-367.

电刺激促进大鼠脊髓钝挫伤后运动功能恢复

曾茜¹, 詹琪¹, 高远¹, 崔乃浩¹, 冯鲁乾^2*

1.贵州医科大学, 贵州贵阳 550004;
2.贵州医科大学附属医院神经外科, 贵州贵阳 550004

收稿日期:2019-04-15 修回日期:2019-09-25 出版日期:2020-03-05 发布日期:2020-03-02
通讯作者: ^*fengluqian@126.com

Electric stimulation enhances recovery of motor function in spinal cord contused rats

ZENG Xi¹, ZHAN Qi¹, GAO Yuan¹, CUI Nai-hao¹, FENG Lu-qian^2*

1. Guizhou Medical University, Guiyang 550004;
2. Department of Neurosurgery, the Affiliated Hospital of Guizhou Medical University, Guiyang 550004, China

Received:2019-04-15 Revised:2019-09-25 Online:2020-03-05 Published:2020-03-02
Contact: ^*fengluqian@126.com

摘要/Abstract

摘要： 目的探究神经电刺激对大鼠脊髓顿挫伤(SCC)后的干预效果并探讨相应机制。方法将大鼠分为:假手术组、对照组和实验组。用Allen's法复制大鼠T9脊髓钝挫伤模型。实验组用神经电刺激干预。定期观察大鼠后肢运动功能恢复情况;用免疫组织化学染色技术以及蛋白质印记技术检测神经生长因子和巢蛋白的表达。结果脊髓损伤后,大鼠的运动功能迅速下降。随着时间的推移,实验组大鼠运动功能评分逐渐升高,第7天较对照组明显上调(P＜0.05)。与对照组相比,实验组神经生长因子和巢蛋白表达明显上调(P＜0.05)。结论电刺激可能创造有利于神经元存活和可塑性的微环境,有利于大鼠后肢运动功能的恢复。

关键词: 脊髓损伤, 神经电刺激, 神经生长因子, 巢蛋白

Abstract: Objective To investigate the effect of nerve electric stimulation on spinal cord contused rats and to explore the appropriate mechanism. Methods Rats were divided into sham group, control group and experimental group. T9 rat spinal cord contusion(SCC) model was developed by Allen's method. Experimental group was treated with nerve electric stimulation. After neurobehavioral score assessment, immunohistochemistry and Western blot were used to detect nerve growth factor and nestin. Results After spinal cord injury, score of rats gradually increased,and compared with control group, the experimental group significant increased(P＜0.05) at d 7. The nerve growth factor and nestin expression of experimental group, up-regulated significantly (P＜0.05). Conclusions Nerve electric stimulation may create a microenvironment conducive to neural survival and differentiation, which facilites the recovery of hindlimb motor function in rats.

Key words: spinal cord injury, nerve electrical stimulation, nerve growth factor, nestin

中图分类号:

R247.9

曾茜, 詹琪, 高远, 崔乃浩, 冯鲁乾. 电刺激促进大鼠脊髓钝挫伤后运动功能恢复[J]. 基础医学与临床, 2020, 40(3): 363-367.

ZENG Xi, ZHAN Qi, GAO Yuan, CUI Nai-hao, FENG Lu-qian. Electric stimulation enhances recovery of motor function in spinal cord contused rats[J]. Basic & Clinical Medicine, 2020, 40(3): 363-367.

参考文献

[1]Eckert MJ, Martin MJ. Trauma: Spinal cord injury[J]. Surg Clin North Am,2017,97:1031-1045.
[2]杨枭雄,于前进,秦江,等.脊髓损伤住院患者1027例流行病学分析[J].脊柱外科杂志,2016,14:301-305.
[3]Ahuja CS, Nori S, Tetreault L. Traumatic spinal cord injury-repair and regeneration[J]. Neurosurgery,2017,80: S9-S22.
[4]李向哲,王灿,吴勤峰,等.脊髓损伤后脑源性神经营养因子神经生物学效应的研究进展[J/OL].中国康复理论与实践,2018,2:160-164.
[5]Gilyarov AV. Nestin in central nervous system cells[J]. Neurosci Behav Physiol,2008,38:165-169.
[6]岗卫娟,武晓冬,王芳,等.统编教材中针灸适宜病症的研究[J].中国针灸,2017,37:339-342.
[7]Huang YG, Tao W, Yang SB, et al. Autophagy: novel insights into therapeutic target of electroacupuncture against cerebral ischemia/reperfusion injury[J]. Neural Regen Res, 2019, 14:36-43.
[8]Fu JP, Mo WC, Liu Y, et al. Elimination of the geomagnetic field stimulates the proliferation of mouse neural progenitor and stem cells[J]. Protein & Cell, 2016, 7:624-637.
[9]Zhang J, Li S, Wu Y. Recovery of spinal cord injury following electroacupuncture in rats through enhancement of Wnt/β-catenin signaling[J]. Mol Med Rep, 2017, 16:2185-2190.
[10]Park D, Xiang AP, Mao FF, et al. Nestin is required for the proper self-renewal of neural stem cells[J]. Stem Cells, 2011, 28:2162-2171.
[11]Barua S, Chung JI, Kim AY, et al. Jak kinase 3 signaling in microgliogenesis from the spinal nestin+ progenitors in both development and response to injury[J]. Neuroreport, 2017, 28:929-935.
[12]Kathleen K, Imran S, George S. Targeting neurotrophins to specific populations of neurons: NGF, BDNF, and NT-3 and their relevance for treatment of spinal cord injury[J]. Int J Mol Sci, 2017, 18:548-564.