标准桃金娘油减轻PM2.5致大鼠气道黏液纤毛的损伤

基础医学与临床 ›› 2020, Vol. 40 ›› Issue (3): 356-362.

标准桃金娘油减轻PM2.5致大鼠气道黏液纤毛的损伤

杜宁¹, 刘小强², 段争^3*

1.邯郸市第一医院东区呼吸内科,河北邯郸 056001;
2.河北省环境监测中心,河北石家庄 050051;
3.河北医科大学第二医院呼吸二科,河北石家庄 050000

收稿日期:2018-12-24 修回日期:2019-09-24 出版日期:2020-03-05 发布日期:2020-03-02
通讯作者: ^*duanzheng1971@126.com

Myrtol standardized reduces the damage of PM2.5 exposure on mucosal cilia in rat airway

DU Ning¹, LU Xiao-qiang², DUAN Zheng^3*

1. Departmend of Respiratory,East District,the First Hospital of Handan,Handan 056001;
2. Hebei Province Environmental Monitoring Center, Shijiazhuang 050051;
3. the Second Departmend of Respiratory, the Second Hospital of Hebei Medical University, Shijiazhuang 050000, China

Received:2018-12-24 Revised:2019-09-24 Online:2020-03-05 Published:2020-03-02
Contact: ^*duanzheng1971@126.com

摘要/Abstract

摘要： 目的观察大气细颗粒物(PM2.5)致大鼠气道黏液纤毛清除系统的损伤作用及标准桃金娘油干预的影响。方法将大鼠分成对照组(N组)、PM2.5暴露组(PM组)、标准桃金娘油干预组(PM+CS组),分别予以吸入净化空气、暴露石家庄室外空气(PM2.5浓度的范围29.90 mg/m³~99.65 mg/m³)、标准桃金娘油混悬液300 mg/(kg·d)灌胃。于3和6个月各处死大鼠8只,气管及肺组织行HE和AB-PAS染色;扫描电镜观察纤毛超微结构;计数BALF中细胞及分类;RT-qPCR测定黏蛋白(MUC)5AC mRNA和水通道蛋白(AQP)5 mRNA表达;免疫组化、Western blot测定MUC5AC蛋白表达。结果 PM组肺及气管组织炎性损伤及黏液细胞化生明显;气管纤毛黏连、倒伏及缺失、排列稀疏、紊乱,BALF中白细胞总数、中性粒细胞计数和中性粒细胞百分比显著高于N组(P＜0.05);MUC5AC mRNA相对表达量显著高于N组(P＜0.05);AQP5 mRNA相对表达量显著低于N组(P＜0.05);MUC5AC蛋白表达显著高于N组(P＜0.05)。与PM组相比,标准桃金娘油可减轻上述损伤作用(P＜0.05)。结论标准桃金娘油可通过减轻PM2.5致大鼠的纤毛结构损伤、黏液高分泌和炎性损伤作用,对黏液纤毛清除系统起保护作用。

关键词: PM2.5, 标准桃金娘油, MCC, MUC5AC, AQP5

Abstract: Objective To investigate the damage effect of fine particulate matter(PM2.5)exposure on mucociliary clearance, and analyze the effect of myrtol standardized on it. Methods Rats were divided into control group(group N),PM2.5 exposure group(group PM) and myrtol standardized group(group PM+CS).Rats were respectively given inhalation to filtered clean air indoor, exposure to ambient air of Shijiazhuang city (the range of PM2.5 concentration from 29.90 mg/m³ to 99.65 mg/m³) and gavage of myrtol standardized suspension 300 mg/(kg·d).Eight rats in each group were sacrificed after 3 months and 6 months.The lung and tracheal tissues were stained with HE and AB-PAS.The ultrastructure of cilia was observed by scanning electron microscope.The number of total cells,neutrophil count and classification in the BALF were detected by microscopy.The gene expression level of MUC5AC and AQP5 in pulmonary tissue were measured by real-time PCR. The protein expressional level of MUC5AC in lung tissue was respectively measured by immunohistochemistry and Western blot. Results In the PM groups, inflammatory injury and myxocyte metaplasia of lung and tracheal tissues were obvious, and tracheal cilia were adhesive, lodging and absent, sparse and disordered.The total number of white blood cells and percentage of neutrophils in BALF were significantly higher than those in N groups(P＜0.05).The expression of MUC5AC mRNA and MUC5AC protein in PM groups was higher than that in N groups(P＜0.05),but the relative expression of AQP5 mRNA in PM groups was significantly lower than that in N groups(P＜0.05).Compared with PM group, myrtol standardized can reduce the above damage effect(P＜0.05). Conclusions Myrtol standardized can protect the mucociliary clearance system in rats by reducing the damage of cilia structure, hypersecretion of mucus and inflammatory damage caused by PM2.5.

Key words: PM2.5, myrtol standardized, MCC, MUC5AC, AQP5

中图分类号:

R563.9

杜宁, 刘小强, 段争. 标准桃金娘油减轻PM2.5致大鼠气道黏液纤毛的损伤[J]. 基础医学与临床, 2020, 40(3): 356-362.

DU Ning, LU Xiao-qiang, DUAN Zheng. Myrtol standardized reduces the damage of PM2.5 exposure on mucosal cilia in rat airway[J]. Basic & Clinical Medicine, 2020, 40(3): 356-362.

参考文献

[1]Dominici F, Peng RD,Bell ML,et al.Fine particulate air pollution and hospital admission for cardiovascular and respiratory diseases[J]. JAMA,2006,295:1127-1134.
[2]Miao H,Takamichi I,Yahao R,et al.PM2.5-rich dust collected from the air in Fukuoka,Kyushu,Japan,can exacerbate murine lung eosinophilia[J].Inhal Toxicol,2015,27:287-299.
[3]段争,杜飞燕,袁雅冬,等.细颗粒物暴露对大鼠肺脏清除肺炎克雷伯菌的影响[J].中华结核和呼吸杂志,2013,36:836-840.
[4]鲍志坚,胡旭,沈晓强,等.PM2.5氧化应激对变应性哮喘气道上皮固有免疫调控因子表达的刺激作用[J].浙经医学,2018,40:142-144.
[5]Begrow F, Böckenholt C, Ehmen M,et al.Effect of myrtol standardized and other Substances on the respiratory tract:ciliary beat frequency and mucociliary clearance as parameters[J].Adv Ther,2012,29:350-358.
[6]Sengespeik HC,Zimmermann T,Peiske C,et al.Myrtol standardized in the treatment of acute and chronic respiratory infections in children.A multicenter post-marketing surveillance study[J].Arzneimittelforschung,1998,48:990-994.
[7]Ferreira-Ceccato AD,Ramos EM,det al.Short terms effects of air pollution from biomass burning in mucociliary clearance of Brazilian sugarcane cutters[J].Respir Med,2011,105:1766-1768.
[8]Miyata R, Eeden SFV.The innate and adaptive immune response induced by alveolar macrophages exposed to ambient particulate matter[J].Toxicol Appl Pharm, 2011,257:209-226.
[9]Wagner JG,Morishital M,Keeler GL,et al.Divergent effects of urban particulate air pollution on allergic airway responses in experimental asthma:acomparison of field exposure studies[J].Environ Health,2012,11:45.doi:10.1186/1476-069X-11-45.
[10]Saunders V,Breysse P,Clark J,et al.Particulate matter-induced airway hyperresponsiveness is lymphocyte depen-dent[J].Environ Health Perspect,2010,118:640-646.
[11]Val S,Belade E, George I,et al. Fine PM induce airway MUC5AC expression through the autocrine effect of amphiregulin[J].Arch Toxicol,2012,86:1851-1859.
[12]Wang K,Feng YL,Wen FQ,et al.Decreased expression of human aquaporin-5 correlated with mucus overproduction in airways of chronic obstructive pulmonarydisease[J].Acta Pharmacol Sin,2007,28:1166-1174.
[13]阮军,缪李丽.香烟烟雾暴露对支气管哮喘大鼠肺组织水通道蛋白5表达的影响[J].四川动物,2013,6:916-921.