重组新城疫病毒 IL29 抑制人胃癌细胞系BGC的增殖与迁移

基础医学与临床 ›› 2020, Vol. 40 ›› Issue (3): 368-373.

重组新城疫病毒 IL29 抑制人胃癌细胞系BGC的增殖与迁移

尹超云¹, 张垚², 陈振威², 张安伟², 张旋风², 张日亭², 步雪峰^3*

1.江苏大学附属医院血管外科, 江苏镇江 212000;
2.江苏大学医学院,江苏镇江 212013;
3.江苏大学附属人民医院普外科, 江苏镇江 212002

收稿日期:2019-03-08 修回日期:2019-09-25 出版日期:2020-03-05 发布日期:2020-03-02
通讯作者: ^*xuefengbu05@163.com

rL-IL29 inhibits proliferation and migration of human gastric cancer cell line BGC

YIN Chao-yun¹, ZHANG Yao², CHEN Zhen-wei², ZHANG An-wei², ZHANG Xuan-feng², ZHANG Ri-ting², BU Xue-feng^3*

1. Department of Vascular Surgery,the Affiliated Hospital of Jiangsu University,Zhenjiang 212000;
2. School of Medicine,Jiangsu University, Zhenjiang 212013;
3. Department of General Surgery,the Affiliated People's Hospital of Jiangsu University,Zhenjiang 212002,China

Received:2019-03-08 Revised:2019-09-25 Online:2020-03-05 Published:2020-03-02
Contact: ^*xuefengbu05@163.com

摘要/Abstract

摘要： 目的探讨表达IL-29的重组新城疫病毒LoSota株(rL-IL29)对人胃癌BGC细胞增殖及迁移的影响及其机制。方法将体外培养的人胃癌BGC细胞分为对照组、rL-IL29组和LoSota株新城疫病毒(NDV)组,分别经PBS、rL-IL29及NDV感染处理后进行下一步实验。蛋白质印迹检测BGC细胞中IL-29、NDV、P-ERK、MMP2、p-AKT蛋白的表达;CCK8法、划痕试验及Transwell小室法侵袭实验检测胃癌BGC细胞的增殖、迁移能力。结果 IL-29蛋白仅在rL-IL29组表达,NDV蛋白在IL-29组及NDV组均稳定表达,且明显高于PBS组(P＜0.05);rL-IL29感染BGC细胞后BGC细胞的增殖及迁移能力明显减弱,且rL-IL29的抑制较NDV组作用更强;BGC细胞经rL-IL29感染后P-ERK、MMP2(66 ku/72 ku)、p-AKT蛋白表达较对照组及NDV组明显减弱(P＜0.05)。结论 rL-IL29抑制胃癌BGC细胞的增殖、侵袭及迁移可能与ERK,AKT等信号通路相关。

关键词: 重组新城疫病毒, 白细胞介素-29, 胃癌BGC细胞, 增殖, 迁移

Abstract: Objective To investigate the effect of recombinant Newcastle disease virus expressing IL-29(rL-IL29) on the proliferation and migration with invasion of gastric cancer cells and to explore the potential mechanism. Methods Gastric cancer cell lines BGC were cultured and divided into the PBS, rL-IL29 and newcastle disease virus(NDV)groups infected by the PBS, rL-IL29 or NDV.Western blot was used to detecte the expression of NDV, IL-29,p-ERK,MMP2 and p-AKT protein in cells.The proliferation,migration and invasion of gastric cancer cell lines BGC were detected by CCK8、cell clone forming experiments、scratch test and Transwell invasion test. Results IL-29 protein was only expressed in rL-IL29 group,NDV protein were expressed both in rL-IL29 group and NDV group and was significantly higher than those of PBS group (P＜0.05). The proliferation and migration of BGC cells infected with rL-IL29 were inhibited more seriously than those infected with NDV or PBS group.The expression of p-ERK,MMP2 (66 ku/72 ku),p-AKT and P65 protein was all significantly decreased(P＜0.05). Conclusions The proliferation and migration of gastric cancer cell lines BGC were inhibited by rL-IL29 and the mechanism may be related with ERK,AKT signaling pathways.

Key words: recombinant Newcastle disease virus, IL-29, gastric cancer cell line BGC, proliferation, migration

中图分类号:

R735.2

尹超云, 张垚, 陈振威, 张安伟, 张旋风, 张日亭, 步雪峰. 重组新城疫病毒 IL29 抑制人胃癌细胞系BGC的增殖与迁移[J]. 基础医学与临床, 2020, 40(3): 368-373.

YIN Chao-yun, ZHANG Yao, CHEN Zhen-wei, ZHANG An-wei, ZHANG Xuan-feng, ZHANG Ri-ting, BU Xue-feng. rL-IL29 inhibits proliferation and migration of human gastric cancer cell line BGC[J]. Basic & Clinical Medicine, 2020, 40(3): 368-373.

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