雌激素促进星形胶质细胞系U-87增殖和侵袭

基础医学与临床 ›› 2020, Vol. 40 ›› Issue (3): 305-309.

雌激素促进星形胶质细胞系U-87增殖和侵袭

刘艳敏^1,2, 费以琳¹, 黎星淼¹, 王宁¹, 陈沫¹, 江秀秀^1*

1.浙江大学医学院附属妇产科医院妇科, 浙江杭州 310006;
2.海宁市中心医院妇产科, 浙江海宁 314408

收稿日期:2019-04-18 修回日期:2019-09-24 出版日期:2020-03-05 发布日期:2020-03-02
通讯作者: ^* jiangxiuxiu0418@zju.edu.cn
基金资助:
浙江省自然科学基金(LY17H040003)

Estrogen promotes proliferation and invasion of astrocyte cell line U-87

LIU Yan-min^1,2, FEI Yi-lin¹, LI Xing-miao¹, WANG Ning¹, CHEN Mo¹, JIANG Xiu-xiu^1*

1.Department of Gynecology, Women's Hospital, School of Medicine, Zhejiang University, Hangzhou 310006;
2. Department of Obstetrics and Gynecology, Central Hospital of Haining, Haining 314408, China

Received:2019-04-18 Revised:2019-09-24 Online:2020-03-05 Published:2020-03-02
Contact: ^* jiangxiuxiu0418@zju.edu.cn

摘要/Abstract

摘要： 目的探究雌激素与星形胶质细胞增殖和侵袭的相关性。方法体外培养星形胶质细胞系U-87,分别进行0、10^－12、10^－10、10^－8和10^－6 mol/L雌激素处理,通过qPCR、Western blot、ELISA、CCK8法以及Transwell小室法分析相关基因、蛋白质表达以及细胞增殖和侵袭功能。结果相比对照组,雌激素呈浓度依赖性降低细胞因子IL-1β、TNF-α、IL-6的表达,升高NGF、PGE2表达(P＜0.05),抑制水通道蛋白2(AQP2)的表达(P＜0.05);同时相比对照组,雌激素呈浓度依赖性促进星形胶质细胞的增殖和侵袭(P＜0.05),细胞活性显著提高。结论雌激素下调AQP2的表达,下调细胞因子的表达,升高NGF、PGE2表达,促进星形胶质细胞的增殖和侵袭。

关键词: 雌激素, 星形胶质细胞, 水通道蛋白2, 增殖

Abstract: Objective To explore the relationships between the estrogen and astrocyte cell proliferation as well as invasion. Methods Astrocyte cell line U-87 was cultured in vitro and treated with 0, 10^－12, 10^－10, 10^－8 and 10^－6 mol/L estrogen. The gene and protein expressions were analyzed by qPCR, Western blot, ELISA; and the cell proliferation and invasion was analyzed by CCK-8 kits and transwell experiments. Results Estrogen significantly inhibited IL-1, TNF-α, IL-6 expression and promoted the expression of NGF and PGE2 (P＜0.05). Furthermore, estrogen inhibited aquaporin 2(AQP2) expression (P＜0.05). The proliferation and invasion experiments showed that estrogen promoted the U-87 cell proliferation and invasion (P＜0.05). Conclusions Estrogen down-regulates the expression of inflammatory factors, inhibites the expression of AQP2, promotes the proliferation and invasion of U-87 cells.

Key words: estrogen, astrocyte, aquaporin 2, proliferation

中图分类号:

刘艳敏, 费以琳, 黎星淼, 王宁, 陈沫, 江秀秀. 雌激素促进星形胶质细胞系U-87增殖和侵袭[J]. 基础医学与临床, 2020, 40(3): 305-309.

LIU Yan-min, FEI Yi-lin, LI Xing-miao, WANG Ning, CHEN Mo, JIANG Xiu-xiu. Estrogen promotes proliferation and invasion of astrocyte cell line U-87[J]. Basic & Clinical Medicine, 2020, 40(3): 305-309.

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