关键词: 白藜芦醇, 静脉移植物再狭窄, mTORC2, Rictor

Abstract: Objective: To investigate the effect of resveratrol on the proliferation, migration and angiogenic ability of HUVECs mediated by Rictor over-expression adenovirus. Methods: The Rictor was obtained through PCR and cloned into GV314 plasmid to construct recombinant plasmid, then co-transfected 293T cells with helper plasmids to obtain Rictor overexpressing adenoviral particles (Ad-Rictor), the vector without target gene Ad-Null was set as the negative control group. Ad-Rictor and Ad-Null were infected HUVECs respectively, we also set up blank control group and resveratrol-intervention group (Ad-Rictor + Res). The expression of recombinant protein was detected by fluorescence microscopy and Western blot. CCK-8 assay, Wound Healing and Matrigel assay were performed to assess the proliferation, migration and tube formation of HUVECs. Result: We constructed Ad-Rictor and Ad-Null successfully, they could infect HUVECs and express Rictor protein efficiently. Ad-Rictor could significantly improve the proliferation, migration and lumen formation ability of HUVECs in vitro compared with control (P<0.05), resveratrol intervention could significantly inhibit these abilities induced by Ad-Rictor (P<0.05). Conclusions: Resveratrol inhibits the proliferation, migration and angiopoietic ability of HUVECs through targeting mTORC2/Rictor.

Key words: resveratrol, vein graft reocclusion, mTORC2, Rictor