关键词: 哺乳动物不育系20样激酶1, 宫颈癌, 增殖, 迁移, 侵袭

Abstract: Objective To discuss the influence of MST1 (mammalian sterile 20-like kinase 1，MST1) on the proliferation，migration and invasion of SiHa cervical cancer. Methods Western blot was used to detect the expression of MST1 in cervical epithelial cells H8 and cervical cancer cells SiHa; PJ3H-HA-MST1 was constructed and transfected it to SiHa cell by LipofectamineＴＭ3000; MST1、 HA、Ki-67 and MMP9 protein expression were evaluated by Western blot; While the proliferation，migration and invasion of SiHa cell were assessed by MTS、scratch adhesion test and Transwell assay respectively. Results Compared SiHa cells with H8 cells，MST1 expression in SiHa cells was significantly lower than that of H8 cells．The plasmid was successfully transfected into SiHa cells, MST1 expression was significantly higher, while the expression of Ki-67 and MMP9 were sharply lower, and the proliferation、migration and invasion ability were all suppressed significantly. Conclusions Overexpression of MST1 can inhibit the proliferation、migration and invasion of cervical cancer cell line SiHa.

Key words: mammalian sterile 20-like kinase 1, cervical cancer, proliferation, migration, invasion