关键词: 突触核蛋白γ, 膀胱癌, 5637细胞系, RNA干扰

Abstract: Objective To explore the effects of γ-synuclein (SNCG) on the proliferation and invasiveness of 5637 bladder cancer cells. Methods Three pairs of SNCG-specific siRNAs were designed and transfected into the 5637 cell lines, then the SNCG expression of the three siRNAs were assessed using RT-PCR and Western blot analysis, while the cell proliferation and invasiveness of the 5637 cells were evaluated by cell counting kit-8 (CCK-8) assay and transwell assay, respectively. Results The bladder cancer cell line 5637 was chose to use in next step due to its highest SNCG expression than that in cell lines EJ, T24 and UMUC-3 (P＜0.05). Compared with NC and empty vector controls, all three SNCG siRNAs were observed to significantly inhibit SNCG expression (P＜0.05), among which the lowest SNCG expression was detected in SNCG-siRNA-244. The proliferation and invasiveness of 5637 cell lines were both suppressed (P＜0.05). Conclusions The proliferation and invasiveness of the bladder cancer cells could be decreased by specifically interferring the SNCG expression, SNCG siRNA may become as a novel target for biomedical therapy in bladder cancer.

Key words: Synuclein γ, bladder cancer, 5637 cell lines, RNA interference