Circ-MALAT1靶向miR-101抑制膀胱癌细胞系SW780侵袭和迁移

doi:10.16352/j.issn.1001-6325.2022.03.020

摘要/Abstract

摘要： 目的探讨circ-MALAT1靶向miR-101抑制膀胱癌细胞侵袭和迁移。方法将膀胱癌细胞系SW780分为si-NC组(转染si-NC)、si-circ-MALAT1组(转染si-circ-MALAT1)、miR-NC组(转染miR-NC)、miR-101组(转染miR-101 mimics)、(si-circ-MALAT1+anti-miR-NC)组(共转染si-circ-MALAT1和anti-miR-NC)、(si-circ-MALAT1+anti-miR-101)组(共转染si-circ-MALAT1和anti-miR-101)。RT-qPCR检测circ-MALAT1和miR-101表达;Transwell小室法检测细胞侵袭和迁移;Western blot检测基质金属蛋白酶2(MMP-2)、基质金属蛋白酶9(MMP-9)、磷酸化磷脂酰肌醇3激酶(p-PI3K)、磷酸化蛋白激酶B(p-AKT)蛋白表达;双荧光素酶报告基因实验检测circ-MALAT1和miR-101的靶向关系。结果与癌旁组织比较,膀胱癌组织中circ-MALAT1表达水平明显上调(P＜0.05),miR-101表达水平明显下调(P＜0.05)。与si-NC组比较,si-circ-MALAT1组中circ-MALAT1表达水平明显下调(P＜0.05),侵袭、迁移细胞数明显下调(P＜0.05),p-PI3K、p-AKT蛋白表达明显下调(P＜0.05)。与miR-NC组比较,miR-101组中miR-101表达水平明显上调(P＜0.05),侵袭、迁移细胞数明显下调(P＜0.05)。与(si-circ-MALAT1+anti-miR-NC)组比较,(si-circ-MALAT1+anti-miR-101)组细胞侵袭、迁移细胞数明显上调(P＜0.05),p-PI3K、p-AKT蛋白表达明显上调(P＜0.05)。结论降低circ-MALAT1表达并上调miR-101抑制膀胱癌细胞系SW780的侵袭和迁移,其机制可能与PI3K/AKT信号通路有关。

关键词: circ-MALAT1, miR-101, 膀胱癌, PI3K/AKT信号通路, 侵袭

Abstract: Objective To explore the function of circ-MALAT1 targeting miR-101 in the inhibition of the invasion and migration of bladder cancer cells. Methods Bladder cancer cell line SW780 was divided into si-NC group (transfection si-NC), si-circ-MALAT1 group (transfection si-circ-MALAT1), miR-NC group (transfection miR-NC), miR-101 group (transfection miR-101 mimics), si-circ-MALAT1+anti-miR-NC group(co-transfection si-circ-MALAT1 and anti-miR-NC)and si-circ-MALAT1+anti-miR-101 group(co-transfection si-circ-MALAT1 and anti-miR-101). RT-qPCR was used to examine the expression of circ-MALAT1 and miR-101; Transwell method was used to detect cell invasion and migration; The matrix metalloproteinase 2 (MMP-2) and matrix metalloproteinase 9 (MMP-9),phosphorylated phosphatidylinositol 3-kinase (p-PI3K), phosphorylated protein kinase B(p-AKT) protein expression were measured by Western blot; Dual luciferase reporter gene experiment was applied to detect the targeting relationship between circ-MALAT1 and miR-101. Results Compared with adjacent tissues, the expression of circ-MALAT1 in bladder cancer tissue was significantly increased (P＜0.05)and the expression of miR-101 was significantly decreased (P＜0.05). Compared with the si-NC group, the expression of circ-MALAT1 in the si-circ-MALAT1 group was significantly reduced(P＜0.05)and the counting number of invasion and migrating cells was significantly reduced (P＜0.05), the expression of p-PI3K and p-AKT protein was significantly reduced(P＜0.05). Compared with the miR-NC group, the expression of miR-101 in the miR-101 group was significantly increased(P＜0.05)and the number of invasion as well as migrating cells were significantly reduced(P＜0.05).Compared with the (si-circ-MALAT1+anti-miR-NC)group, the(si-circ-MALAT1+anti-miR-101)group showed more cell invasion and migration (P＜0.05), the expression of p-PI3K and p-AKT protein was significantly increased(P＜0.05). Conclusions Reduction of circ-MALAT1 expression and up-regulation miR-101 inhibit the invasion and migration of bladder cancer cells line SW780. The mechanism may be related to the PI3K/AKT signaling pathway.

Key words: circ-MALAT1, miR-101, bladder cancer, PI3K/AKT signaling pathway, invasion

中图分类号:

R737.1

杨芒庄, 杨旭东, 王晓龙. Circ-MALAT1靶向miR-101抑制膀胱癌细胞系SW780侵袭和迁移[J]. 基础医学与临床, 2022, 42(3): 472-478.

YANG Mang-zhuang, YANG Xu-dong, WANG Xiao-long. Circ-MALAT1 targeting miR-101 inhibits invasion and migration of bladder cancer cell line SW780[J]. Basic & Clinical Medicine, 2022, 42(3): 472-478.

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