基础医学与临床 ›› 2023, Vol. 43 ›› Issue (9): 1359-1363.doi: 10.16352/j.issn.1001-6325.2023.09.1359

• 研究论文 • 上一篇    下一篇

培美曲塞抑制人肺腺癌细胞系A549增殖

潘闪*, 林铿强, 陈树兴   

  1. 福建省福州肺科医院 胸外科, 福建 福州 350000
  • 收稿日期:2022-06-06 修回日期:2023-02-20 出版日期:2023-09-05 发布日期:2023-09-01
  • 通讯作者: *panshanfj83@126.com
  • 基金资助:
    福建省科技计划(2018D0007)

Pemetrexed inhibits the proliferation of lung adenocarcinoma cell line A549

PAN Shan*, LIN Kengqiang, CHEN Shuxing   

  1. Department of Thoracic Surgery,Fuzhou Pulmonary Hospital of Fujian Province,Fuzhou 350000, China
  • Received:2022-06-06 Revised:2023-02-20 Online:2023-09-05 Published:2023-09-01
  • Contact: *panshanfj83@126.com

摘要: 目的 探究培美曲塞是否通过抑制PI3K/AKT信号通路调控肺腺癌细胞增殖。方法 选取人肺腺癌细胞系A549进行细胞复苏、培养及传代,分为对照组、低和高剂量培美曲塞干预组。CCK-8法检测A549细胞增殖率;流式细胞仪检测检测细胞周期和凋亡率;Western blot测定凋亡相关蛋白质和PI3K/AKT信号通路蛋白质的差异。结果 随着药物剂量和处理时间的增加,对A549细胞的增殖抑制率明显增高(P<0.05);A549细胞的凋亡率也逐渐上升,(P<0.05或P<0.01);Cleaved caspase-3和Bad蛋白表达水平显著上升,而Bcl-2蛋白表达水平显著降低(P<0.05或P<0.01)。在培养48 h时,随着培美曲塞药物浓度的增加,A549细胞G0/G1期比例明显增加,而S期和G2/M 期比例明显降低(P<0.05或P<0.01);PI3K、AKT和p-AKT蛋白表达水平显著降低(P<0.05)。结论 培美曲塞可抑制肺腺癌细胞系A549增殖,促进凋亡,其机制可能是通过抑制PI3K/AKT信号通路进行调控的。

关键词: 培美曲塞, PI3K/AKT信号通路, 肺腺癌, 细胞增殖

Abstract: Objective To investigate whether pemetrexed can regulate the proliferation of lung adenocarcinoma cells. Methods Human lung adenocarcinoma cell line A549 was selected for cell resuscitation, culture and passage, and was divided into control group, low-dose and high-dose pemetrexed intervention group. CCK-8 assay was used to detect proliferation inhibition rate of A549 cells. Flow cytometry was used to detect cell cycle and apoptosis; Western blot was used to detect the difference between proteins related to apoptosis and to PI3K/AKT signal pathway function. Results With the increase of drug dose and treatment time, both inhibition rate to A549 cells proliferation and apoptosis were significantly increased.The expression of cleaved caspase-3 and Bad protein was also significantly increased while the expression of Bcl-2 protein was significantly decreased (P<0.05 or P<0.01). At 48 h of culture, with the increase of pemetrexed concentration, the proportion of G0/G1 phase of A549 cells increased significantly, while the proportion of S phase and G2/M phase decreased significantly (P<0.05 or P<0.01);The expression of PI3K, AKT and p-AKT wassignificantly decreased (P<0.05 or P<0.01). Conclusions Pemetrexed can inhibit the proliferation and promote apoptosis of lung adenocarcinoma cell line A549, potentially through a mechanism of inhibiting PI3K/AKT signaling pathway.

Key words: pemetrexed, PI3K/AKT signal pathway, lung adenocarcinoma, cell proliferation

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