基础医学与临床 ›› 2012, Vol. 32 ›› Issue (6): 682-686.

• 研究论文 • 上一篇    下一篇

细胞融合通过PI3K-AKT通路降低黑色素瘤细胞增殖能力

米蕊芳1,潘春晓1,卞晓翠1,田文嘉2,宋利强1   

  1. 1. 中国医学科学院 北京协和医学院 基础医学研究所
    2. 中国医学科学院基础医学研究所
  • 收稿日期:2012-03-21 修回日期:2012-04-25 出版日期:2012-06-05 发布日期:2012-05-25
  • 通讯作者: 米蕊芳 E-mail:mrf5560@gmail.com

Cell fusion reduced melanoma cell proliferation through PI3K-AKT pathway

  • Received:2012-03-21 Revised:2012-04-25 Online:2012-06-05 Published:2012-05-25

摘要: 摘要:目的 研究细胞融合在黑色素瘤细胞增殖中的作用及其分子机制。方法 用聚乙二醇细胞融合的方法将黑色素瘤细胞融合,并通过流式分选获得稳定的融合细胞。体外培养及细胞计数检测融合细胞的增殖速度。Affymatrix基因芯片分析黑色素瘤细胞融合前后差异表达的基因,并用Ingenuity Pathways Analysis (IPA)软件进行基因功能分析。Western blot方法检测细胞蛋白表达。结果 PEG化学融合法成功获得稳定的黑色素瘤融合细胞。融合细胞的体外增殖速度明显减慢。进一步的基因芯片及基因功能分析显示黑色素瘤融合细胞与增殖相关的PI3K-AKT通路明显下调。融合细胞phospho-S6蛋白表达明显降低。 结论 细胞融合通过PI3K-AKT通路降低黑色素瘤细胞增殖能力。

关键词: 细胞融合, 黑色素瘤, 增殖

Abstract: Abstract: Objective To investigate the effect of cancer cell fusion in melanoma proliferation and the underlying mechanism. Methods Stable melanoma fusion cells were obtained by the polyethylene glycol (PEG) fusion method and isolated by the fluorescence activated cell sorting (FACS). Cell proliferation rate was detected by cell culture in vitro and counting the cell number everyday. Differentially expressed genes between the stable melanoma fusion hybrids and the parental cells were detected by the Affymatrix gene chip following the gene function analysis by Ingenuity Pathways Analysis (IPA). Protein expression was analyzed by western blot. Results Stable melanoma fusion cells were successfully obtained by the PEG fusion method. The proliferation rate of fusion hybrids was significantly decreased compared to the parental melanoma cells. Gene chip and the following gene function analyses showed that PI3K-AKT pathway related to cell proliferation was significantly down-regulated in the melanoma fusion hybrids. Moreover, the expression of phospho-S6 in the fusion hybrids significantly decreased. Conclusions Cell fusion reduced melanoma cell proliferation through the PI3K-AKT pathway.

Key words: cell fusion, melanoma, proliferation

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