基础医学与临床 ›› 2011, Vol. 31 ›› Issue (9): 976-979.

• 研究论文 • 上一篇    下一篇

重组腺病毒Ad-ING4和Ad-PTEN对肝癌细胞的抑制作用

姚雷1,曹婧媛1,吴鹏飞1,黄玉洪1,缪竞诚2,王金志3,杨吉成3   

  1. 1. 苏州大学附属第一人民医院
    2. 苏州大学
    3.
  • 收稿日期:2010-09-27 修回日期:2010-12-02 出版日期:2011-09-05 发布日期:2011-09-05
  • 通讯作者: 缪竞诚 E-mail:mjc@suda.edu.cn
  • 基金资助:
    国家级大学生创新性实验计划项目

Investigation of inhibition of adenovirus mediated gene ING4 and PTEN to hepatocarcinoma Cells

  • Received:2010-09-27 Revised:2010-12-02 Online:2011-09-05 Published:2011-09-05

摘要: [摘要] 目的:用构建好的重组腺病毒Ad-ING4和Ad-PTEN感染人肝癌SMMC-7721细胞,检验对细胞的作用。方法:用构建好的重组腺病毒Ad-ING4和Ad-PTEN感染QBI-293A细胞,对病毒进行扩增并测定其效价,荧光显微镜下观察感染效果。用扩增得到的Ad-ING4和Ad-PTEN感染SMMC-7721细胞,流式细胞术及MTT检测作用效果。结果:空腺病毒组的凋亡率为3.4±1.3%,Ad-ING4组和Ad-PTEN组分别为49.7±4.8%和11.4±3.2%,均显著高于空腺病毒组(p<0.01);Ad-ING4组和Ad-PTEN组的细胞增殖在48h~96h时较正常组明显下降(p<0.05)。结论:Ad-ING4和Ad-PTEN对SMMC-7721细胞均有显著抑制作用, 尤其是Ad-ING4。

关键词: 肝癌, Ad-ING4, Ad-PTEN, 基因

Abstract: [Abstract] Purpose: We use recombinant Ad-ING4 and Ad-PTEN to infect human hepatocarcinoma cell line SMMC-7721 in vitro, test and compare the effects of gene expression of the cells. Method: We use recombinant Ad-ING4 and Ad-PTEN and control virus to infect QBI-293A cells for viral propagation and viral titer detection. We confirm the infective effects of Ad-ING4, Ad-PTEN and control virus by fluorescence observation. Use Ad-ING4 and Ad-PTEN to infect SMMC-7721 cells and confirm their effects by their apoptotic rate and the results of MTT. Results: The apoptotic rate of control virus group is 3.4±1.3%, the Ad-ING4 group is 49.7±4.8%(p<0.01)and the Ad-PTEN group is 11.4±3.2%(p<0.01). The cell proliferation of group Ad-ING4 and Ad-PTEN obviously decreased from 48h to 96h(p<0.05).Conclusion: The data analysis showed Ad-ING4 and Ad-PTEN had an inhibitory effect to SMMC-7721 cells, and the effect of Ad-ING4 is more severe.

Key words: hepatocarcinoma, Ad-ING4 , Ad-PTEN, gene

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