基础医学与临床 ›› 2011, Vol. 31 ›› Issue (8): 900-904.

• 研究论文 • 上一篇    下一篇

多肿瘤标记物蛋白芯片对胆管癌诊断的临床评价

何江1,丁剑冰1,余伍忠2,邹红云2   

  1. 1. 新疆医科大学基础医学院
    2. 兰州军区乌鲁木齐总医院临床医学研究所
  • 收稿日期:2010-07-02 修回日期:2010-08-24 出版日期:2011-08-05 发布日期:2011-07-13
  • 通讯作者: 丁剑冰 E-mail:djbing002@163.com

Diagnostic value of multiple tumor marker protein biochip for patients with cholangiocarcinoma

Jiang HE1,Jian-bing DING1,Wu-zhong YU2,Hong-yun ZOU2   

  1. 1. Xinjiang Medical University
    2.
  • Received:2010-07-02 Revised:2010-08-24 Online:2011-08-05 Published:2011-07-13
  • Contact: Jian-bing DING E-mail:djbing002@163.com

摘要: 目的 探讨多肿瘤标记物蛋白芯片(C-12)对胆管癌的诊断价值。 方法 用C-12同时检测26例胆管癌初治患者、52例消化系统良性疾病初治患者和65例健康体检者血清中CA199、CEA、AFP、CA125、Ferritin、β-HCG、HGH、CA153、CA242、PSA、f-PSA和NSE共12项标志物的表达,筛选出与胆管癌相关性最强的肿瘤标志物及肿瘤标志物最佳联合检测组合,并盲法验证最佳检测组合的诊断价值。 结果 胆管癌组中CA199、CEA、CA242、Ferritin、AFP、 CA125和CA153检测阳性率显著高于良性疾病组和正常对照组(P<0.01), CA199、NSE、CA242、Ferritin、β-HCG、AFP、CA125、HGH和CA153的表达水平显著高于良性疾病组和正常对照组 (P<0.05~0.01),与胆管癌诊断相关性最强的肿瘤标记物是CA199,检测敏感性、特异性和准确性分别为69.2%、96.9%和89.0%,胆管癌的最佳3项组合与C-12相比,敏感性稍低,特异性和准确性稍高,但差异均无统计学意义,盲法验证最佳3项组合CA199+CA125+CEA对于胆管癌诊断的敏感性、特异性和准确性分别为91.7%、87.5%和89.6%。 结论 C-12对胆管癌具有较高的诊断价值,最佳3项组合CA199+CA125+CEA足以替代C-12的12项肿瘤标志物联合检测,可能是一种既经济又有效的胆管癌诊断标志物组合。

关键词: 肿瘤标记物, 蛋白质阵列分析, 诊断, 胆管癌

Abstract: Objective To evaluate the diagnostic value of multiple tumor markers protein biochip (C-12) for patients with cholangiocarcinoma. Methods The serum levels of 12 tumor markers (TMs), including CA199, NSE, CEA, CA242, Ferritin, β-HCG, AFP, f-PSA, PSA, CA125, CA153 and HGH, were measured in 26 cholangiocarcinoma patients, 52 benign disease patients of digestive system and 65 normal controls. The most relevant TM and the combinations of TMs to the improvement of diagnosis were determined. Results The positive rates of CA199, CEA, CA242, Ferritin, AFP, CA125 and CA153 in the cholangiocarcinoma patients had significantly higher than those of the benign disease patients and normal controls (P<0.01). The serum levels of CA199, NSE, CA242, Ferritin, β-HCG, AFP, CA125, HGH and CA153 in the cholangiocarcinoma patients had significantly higher than those of the benign disease patients and normal controls (P<0.05~0.01). Among all the 12 TMs, the single best TM was CA199 and gave the sensitivity, specificity and validity of 69.2%, 96.9% and 89.0% for the cholangiocarcinoma, respectively.The best combination of 3 TMs (CA199+CA125+CEA) had not statistically significant comparied with C-12 and yielded a sensitivity of 91.7%, a specificity of 87.5% and a validity of 89.6%. Conclusions The multiple tumor markers protein biochip has a relatively high value in the diagnosis of cholangiocarcinoma. The combination detection of 3 TMs (CA199+CA125 +CEA) could replace 12 TMs of C-12 and may be the economical and effective in the diagnosis of cholangiocarcinoma.

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