基础医学与临床 ›› 2011, Vol. 31 ›› Issue (11): 1242-1246.

• 研究论文 • 上一篇    下一篇

干预离子通道联合密度梯度离心富集胎儿有核红细胞的研究

刘芳1,张丽娜2,徐向红2,程宁2,白亚娜3   

  1. 1. 兰州大学第一医院
    2. 兰州大学生殖健康与出生缺陷研究中心
    3. 兰州大学流行病与卫生统计学研究所
  • 收稿日期:2010-08-27 修回日期:2011-03-08 出版日期:2011-11-05 发布日期:2011-11-02
  • 通讯作者: 程宁 E-mail:chengn@lzu.edu.cn
  • 基金资助:
    01CX-03,02CX-04;省重点公关项目

Enriching the Fetal Nuclear Red Blood Cells by Intervening

  • Received:2010-08-27 Revised:2011-03-08 Online:2011-11-05 Published:2011-11-02

摘要: 摘要:目的 探讨干预膜表面K-Cl 同向转运体(KCC)离子通道联合密度梯度离心对富集脐血中胎儿有核红细胞(NRBC)的效果。方法 应用KCC特异激活剂尿素(Urea)干预细胞膜,建立最大限度缩小细胞体积的干预条件;应用最佳干预条件干预脐血后进行不同密度介质下离心富集NRBC,用流式细胞仪计数观察胎儿NRBC的富集效果。结果 比较传统1.077淋巴细胞分离液离心后的单个核层流式细胞仪计数NRBC8.6%,干预脐血后用1.065密度介质离心后下层白膜层达69.9%,未干预组中用1.099密度介质离心的单个核层达55.9%,分别提高富集量8倍和6.5倍。结论 尿素干预联合密度梯度离心能有效提高NRBC的富集率。 关键词:胎儿有核红细胞;离子通道;尿素;干预;流式细胞仪

关键词: 胎儿有核红细胞, 离子通道, 尿素, 干预, 流式细胞仪

Abstract: Abstract:Objective To investigate the effectiveness of the Nucleated Red Blood Cells’ enrichment by intervening membrance ionic channels- K+/Cl- cotransporter and density gradient centrifugation in umbilical cord blood. Methods Setting up optical Intervening condition of maximal change of cell volume from RBCs on the umbilical cord blood, these rates of cell’s enrichment by different density medium centrifugation were determined by flow cytometry. Results Compared to traditional rich method, the average purity of NRBC enrichment in the umbilical cord blood could take from 8.6% to 69.9% and 55.9% by centrifugation of 1.065g/L medium after intervening and 1.099g/L medium,which means to respectively increase about .8 and 6.5 times enrichment of NRBC. Conclusions Both urea intervention and density centrifugation could effectively promote the rate of the NRBC’enrichment .

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