大鼠ARDS基因表达谱中信号传导基因的表达

基础医学与临床 ›› 2009, Vol. 29 ›› Issue (8): 835-839.

大鼠ARDS基因表达谱中信号传导基因的表达

刘秀珍刘凤张如意张秀丽张小华栾海云

滨州医学院药学院

收稿日期:2009-02-11 修回日期:2009-05-19 出版日期:2009-08-20 发布日期:2009-08-20

Expression of signal transduction pathway genes in ARDS transcription profiles of rats

Xiu-zhen LIU, Feng LIU, Ru-yi ZHANG, Xiu-li ZHANG, Xiao-hua ZHANG, Hai-yun LUAN

College of Pharmaceutical,Binzhou Medical University

Received:2009-02-11 Revised:2009-05-19 Online:2009-08-20 Published:2009-08-20

摘要/Abstract

摘要： 目的对大鼠急性呼吸窘迫综合征(ARDS)基因表达谱中的信号传导基因进行研究与分析。方法从正常和ARDS大鼠肺组织中提取总RNA，分离纯化mRNA，经反转录合成掺入生物素标记的cDNA探针，然后与基因芯片杂交，扫描芯片荧光信号图像，用Sam3.0进行统计处理，用博奥生物分子功能注释系统V4.0进行功能分析。随机选择3个差异表达基因 ,用荧光定量RT- PCR验证。结果在ARDS大鼠肺组织中，信号传导相关基因上调的有2个，下调的有9个，涉及到的相关信号通路11个。结论大鼠ARDS基因表达谱中涉及到许多信号传导基因和通路的差异表达。

关键词: ARDS, 基因芯片, 信号传导

Abstract: Objective This study aims to study the signal transduction pathway genes in ARDS(Acute respiratory distress syndrome ) of rats by genechip. Methods The total RNAs were isolated from lungs of normal and ARDS rats respectively. The RNAs were purified by oligotex. Both the mRNAs from two kinds of tissues were reversely transcribed to cDNA with the incorporation of fluorescent-labelled dUTP to prepare the hybridization probes. The mixed probes were hybridized to cDNA microarray. Picture signals of fluorescence in gene array were scanned and compared by CapitlBio Molecule Annotation System V4.0. Quantitative fluorescence RT-PCR was used to validate the results of genechip. Results The results showed that there were 2 genes up-regulated and 9 genes down-regulated over 1.5 times in lung tissues of ARDS rats，and these genes were involved in 11 signal transduction pathways. Conclusion: Many genes of signal transduction pathways expressed differentially in ARDS transcription profiles of rats.

Key words: ARDS, Genechip, signal transduction pathway

刘秀珍刘凤张如意张秀丽张小华栾海云. 大鼠ARDS基因表达谱中信号传导基因的表达[J]. 基础医学与临床, 2009, 29(8): 835-839.

Xiu-zhen LIU; Feng LIU; Ru-yi ZHANG; Xiu-li ZHANG; Xiao-hua ZHANG; Hai-yun LUAN. Expression of signal transduction pathway genes in ARDS transcription profiles of rats[J]. Basic & Clinical Medicine, 2009, 29(8): 835-839.

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