关键词: 壳聚糖, 硫酸镁, PC12细胞, 生物相容性

Abstract: Objective To prepare the chitosan-Mg membranes and explore the biocompatibility of membranes with PC12 cells and feasibility of its usage as biomaterials in tissue engineering. Methods The appearance of the chitosan-Mg membranes (CM) was observed under scanning electromicroscope (SEM) and the element of the membranes was analyzed by x-ray energy spectromete. PC12 cells were co-cultured with CM in vitro, the morphological changes of PC12 cells on membranes were observed under scanning electron microscope (SEM) and light microscope (LM), and the cell vitality was detected by MTT assay. Results: The surface of chitosan membranes (CS) was smoother than that of CM;but the CM groups were full of pore observed under SEM；The content of Mg element was related with the dose of MgSO4 added into chitosan solution in dosage dependence. Morphology observation showed that PC12 cells grew well on CM compared with CS group. The cells were rich of microvilli and long processes on 7th day, and synapses alike structure was formed in many PC12 cells. In addition, the cell viability of experiment group was higher than that of control group（P＜0.05）after 5 days detected by MTT method. Conclusion Chitosan-Mg membrances has good biocompatibility with PC12 cells.

Key words: chitosan, MgSO4, PC12 cells, biocompatibility