关键词: 艾司氯胺酮, 缺血/再灌注损伤, 氧化应激, 核因子E2相关因子2(Nrf2)

Abstract: Objective To investigate the role of low dose esketamine in pretreated limb ischemia-reperfusion injury. Methods The rats were divided into sham-operated group (Sham group), ischemia-reperfusion group (I/R group, 3 h of ischemia and 2 h of reperfusion), esketamine group (ESK group, ip, 5 mg/kg). The plasma concentrations of creatine kinase (CK) and lactate dehydrogenase (LDH) were measured. The wet/dry weight ratio of skeletal muscle was immediately detected. The gastrocnemius muscle was harvested and the level of malondialdehyde (MDA) and superoxide dismutase (SOD) was detected by colorimetric assay. The expression of nuclear factor erythroid 2-related factor 2 (Nrf2) and heme oxygenase-1 (HO-1) were detected by Western blot and immunohistochemical staining. Results Compared with Sham group, the index of wet/dry weight ratio, MDA, CK, LDH, Nrf2 and HO-1 were all increased, but SOD was decreased in I/R group(P＜0.05). Compared with I/R group, the index of wet/dry weight ratio, MDA, CK and LDH were significantly lower, but SOD and Nrf2 and HO-1 were significantly higher in ESK group (P＜0.05). Conclusions ESK may increase Nrf2 in the nucleus, thereby increase the HO-1 protein as an antioxidant agent.

Key words: esketamine, ischemia-reperfusion injury, oxidative stress, Nrf2