摘要:
目的 探讨颅脑创伤后期(第7 天)大鼠脑组织代谢型谷氨酸受体5(mGluR5)表达变化,以及牛磺酸治疗作用。方法 液压脑损伤打击仪制备液压打击颅脑创伤大鼠模型,采用随机数字表法将30 只无特定病原体级Sprague-Dawley 大鼠随机分为对照组、颅脑创伤组和牛磺酸治疗组(各10 只),干湿重法检测大鼠脑组织含水量,实时荧光定量聚合酶链反应和Western blotting 法检测水通道蛋白4(AQP4)和mGluR5 mRNA 和蛋白表达变化。结果 与对照组相比,颅脑创伤组大鼠脑组织含水量(t = 4.893,P = 0.002)、AQP4 mRNA(t = 6.523,P = 0.000)和蛋白(t = 4.366,P = 0.008)表达水平升高,mGluR5 mRNA(t = 5.776,P = 0.001)和蛋白(t = 3.945,P = 0.014)表达水平降低;经牛磺酸治疗后,大鼠脑组织含水量(t = 2.151,P = 0.140)、AQP4 mRNA(t = 1.144,P = 0.432)和蛋白(t = 0.367,P = 0.804)降至正常水平,mGluR5 mRNA(t = 1.824,P = 0.216)和蛋白(t = 1.185,P = 0.414)升至正常水平。相关分析显示,脑组织含水量与mGluR5 mRNA(r = -0.617,P = 0.014)和蛋白(r = -0.665,P = 0.007)呈负相关,与AQP4蛋白呈正相关(r = 0.658,P = 0.008)。结论 牛磺酸可以升高颅脑创伤后期(第7 天)大鼠脑组织mGluR5 表达水平,降低脑水肿和脑组织含水量,具有抑制性神经递质作用。
关键词:
颅脑损伤,
受体, 谷氨酸,
牛磺酸,
疾病模型, 动物
Abstract:
Objective To investigate the change of metabotropic glutamate receptor 5 (mGluR5) expression level in rats with late-stage (the 7th day) traumatic brain injury (TBI) and the role of taurine. Methods The left cerebral TBI rat models were made by using lateral fluid percussion method. A total of 30 specific pathogen free (SPF) male Sprague-Dawley (SD) rats were randomly divided into 3 groups: sham operation group (control group), TBI model group (TBI group) and taurine treatment group (taurine group). Wet and dry weight method was used to measure the brain water content. Real-time fluorescent quantitative polymerase chain reaction (PCR) and Western blotting were used to detect the change of mRNA and protein expression of aquaporin 4 (AQP4) and mGluR5 in each group. Results Compared with control group, the brain water content (t = 4.893, P = 0.002), AQP4 mRNA (t = 6.523, P = 0.000) and protein (t = 4.366, P = 0.008) expression were upregulated, while mGluR5 mRNA (t = 5.776, P = 0.001) and protein (t = 3.945, P = 0.014) expression were downregulated in TBI group. After taurine treatment, the brain water content (t = 2.151, P = 0.140), AQP4 mRNA (t = 1.144,P = 0.432) and protein (t = 0.367, P = 0.804) decreased to normal, while mGluR5 mRNA (t = 1.824, P = 0.216) and protein (t = 1.185, P = 0.414) increased to normal. Correlation analysis showed brain water content was negatively correlated with mGluR5 mRNA (r = -0.617, P = 0.014) and mGluR5 protein (r = -0.665, P = 0.007), while it was positively correlated with AQP4 protein (r = 0.658, P = 0.008). Conclusions Taurine can significantly increase the mGluR5 expression level of brain issue in the late-stage (the 7th day) of TBI and decline brain edema and brain water content. It may be a potential protective agent as an inhibitory neurotransmitter.
Key words:
蔡英, 黄慧玲, 范维佳, 武俏丽. 颅脑创伤后期大鼠代谢型谷氨酸受体5表达变化及牛磺酸治疗作用[J]. 中国现代神经疾病杂志, 2016, 16(8): 503-508.
CAI Ying, HUANG Hui-ling, FAN Wei-jia, WU Qiao-li. The change of metabotropic glutamate receptor 5 expression level in rats with late-stage traumatic brain injury and the therapeutic effect of taurine[J]. Chinese Journal of Contemporary Neurology and Neurosurgery, 2016, 16(8): 503-508.