长链非编码RNA SNORD3A在脑胶质瘤中的表达变化及功能研究

doi:10.3969/j.issn.1672-6731.2019.12.007

中国现代神经疾病杂志 ›› 2019, Vol. 19 ›› Issue (12): 945-953. doi: 10.3969/j.issn.1672-6731.2019.12.007

2 长链非编码RNA SNORD3A在脑胶质瘤中的表达变化及功能研究

慕茂林, 牛万祥, 张晓明, 汤深凤, 胡珊珊, 牛朝诗

230001 合肥, 安徽医科大学附属省立医院神经外科脑功能与脑疾病安徽省重点实验室

收稿日期:2019-12-10 出版日期:2019-12-25 发布日期:2020-01-03
通讯作者: 牛朝诗,Email:niuchaoshi@163.com
基金资助:
脑功能与脑疾病安徽省重点实验室绩效考核补助项目（项目编号：1606c08235）；中央引导地方科技发展专项项目（项目编号：2017070802D144）；安徽省自然科学基金青年科学基金资助项目（项目编号：1908085QH336）

Expression and function of lncRNA SNORD3A in glioma

MU Mao-lin, NIU Wan-xiang, ZHANG Xiao-ming, TANG Shen-feng, HU Shan-shan, NIU Chao-shi

Department of Neurosurgery, Anhui Provincial Hospital Affiliated to Anhui Medical University, Anhui Province Key Laboratory of Brain Function and Brain Disease, Hefei 230001, Anhui, China

Received:2019-12-10 Online:2019-12-25 Published:2020-01-03
Contact: NIU Chao-shi (Email:niuchaoshi@163.com)
Supported by:
This study was supported by Brain Function and Brain Disease Anhui Provincial Key Laboratory Performance Assessment Subsidy Project (No. 1606c08235), the Central Guides Local Science and Technology Development Projects (No. 2017070802D144), and Anhui Natural Science Foundation for Youth Scientists (No. 1908085QH336).

摘要/Abstract

摘要：

目的探讨长链非编码（lncRNA）SNORD3A在胶质瘤组织和胶质瘤细胞系中的表达变化，以及对胶质瘤细胞增殖和侵袭能力的影响。方法采用生物信息学方法分析美国国家生物技术信息中心（NCBI）GEO数据库收录的GSE58276中差异表达的lncRNA，采集2017年6月至2019年8月手术切除的胶质瘤组织标本30例，实时荧光定量聚合酶链反应（PCR）检测lncRNA SNORD3A表达水平；小干扰RNA转染胶质瘤细胞系T98G和U251，CCK-8细胞增殖实验检测胶质瘤细胞增殖能力、Transwell细胞侵袭实验检测胶质瘤细胞侵袭能力、Western blotting法检测胶质瘤细胞c-Myc mRNA和蛋白表达变化。结果与对照组相比，胶质瘤组织lncRNA SNORD3A表达水平升高（P=0.000）；与HEB细胞相比，胶质瘤细胞系T98G、U87、U251和U373 lncRNA SNORD3A表达升高（均P<0.05）。si-SNORD3A-1组和si-SNORD3A-2组T98G细胞（P=0.001，0.007）和U251细胞（P=0.002，0.009）lncRNA SNORD3A表达水平低于对照组；转染后24、48和72 h，si-SNORD3A-1组和si-SNORD3A-2组T98G细胞（均P=0.000）和U251细胞（均P=0.000）增殖能力低于对照组；转染后48 h，si-SNORD3A-1组和si-SNORD3A-2组穿过小室的T98G和U251细胞数目少于对照组（均P=0.000）、c-Myc mRNA和蛋白表达水平低于对照组（均P<0.01）。结论 lncRNA SNORD3A可能通过靶向c-Myc蛋白促进T98G和U251细胞的增殖和侵袭。

关键词: 神经胶质瘤, RNA, 长链非编码, 细胞增殖, 肿瘤侵润, 肿瘤细胞, 培养的

Abstract:

Objective To explore the expression of long non-coding RNA (lncRNA) SNORD3A in glioma tissues and cell lines and its effect on proliferation and invasion of glioma cells. Methods We analyzed the differentially expressed lncRNAs in the National Center for Biotechnology Information (NCBI) GEO (GSE58276) by bioinformatics. A total of 30 glioma tissue specimen were collected from June 2017 to August 2019, lncRNA SNORD3A expression was detected by real-time fluorescence quantitative polymerase chain reaction (PCR). The si-SNORD3A was transfected into T98G and U251 cells. CCK-8 was used to analyze the proliferative capacity of cells. Transwell assay was used to detect cell invasion changes. The expression of c-Myc protein was detected by Western blotting. Results Compared with the control group, the expression level of lncRNA SNORD3A in glioma tissue was increased (P=0.000). Compared with HEB cells, the expression levels of T98G, U87, U251 and U373 lncRNA SNORD3A in glioma cell lines were elevated (P<0.05, for all). The expression levels of lncRNA SNORD3A in T98G cells (P=0.001, 0.007) and U251 cells (P=0.002, 0.009) in si-SNORD3A-1 group and si-SNORD3A-2 group were lower than those in control group. At 24, 48 and 72 h after transfection, the proliferation capacity of T98G cells (P=0.000, for all) and U251 cells (P=0.000, for all) in si-SNORD3A-1 and si-SNORD3A-2 groups were lower than those in control group. At 48 h after transfection, the number of T98G and U251 cells passing through the chamber in si-SNORD3A-1 and si-SNORD3A-2 groups were less than those in control group (P=0.000, for all), and the levels of c-Myc mRNA and protein expression were lower than control group (P<0.01, for all). Conclusions lncRNA SNORD3A promotes proliferation and invasion of T98G and U251 cells by partly targeting c-Myc.

Key words: Glioma, RNA, long noncoding, Cell proliferation, Neoplasm invasiveness, Tumor cells, cultured

慕茂林, 牛万祥, 张晓明, 汤深凤, 胡珊珊, 牛朝诗. 长链非编码RNA SNORD3A在脑胶质瘤中的表达变化及功能研究[J]. 中国现代神经疾病杂志, 2019, 19(12): 945-953.

MU Mao-lin, NIU Wan-xiang, ZHANG Xiao-ming, TANG Shen-feng, HU Shan-shan, NIU Chao-shi. Expression and function of lncRNA SNORD3A in glioma[J]. Chinese Journal of Contemporary Neurology and Neurosurgery, 2019, 19(12): 945-953.

http://journal11.magtechjournal.com/cjcnn/CN/Y2019/V19/I12/945

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Expression and function of lncRNA SNORD3A in glioma

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Expression and function of lncRNA SNORD3A in glioma

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