中国现代神经疾病杂志 ›› 2014, Vol. 14 ›› Issue (7): 615-620. doi: 10.3969/j.issn.1672-6731.2014.07.012

• 临床研究 • 上一篇    下一篇

2 新疆维吾尔自治区维汉两族缺血中风急性期APN基因多态性与中医证候分布特征的关联分析

张震中, 梁晓鹰, 吕光耀, 姚华   

  1. 830054 乌鲁木齐,新疆医科大学公共卫生学院(张震中);830001 新疆医科大学附属中医医院神经内科(梁晓鹰,吕光耀);830054 乌鲁木齐,新疆医科大学第一附属医院党办(姚华)
  • 出版日期:2014-07-25 发布日期:2014-07-27
  • 通讯作者: 姚华 (Email:gzybgzyb@163.com)
  • 基金资助:

    国家自然科学基金资助项目(项目编号:81060289)

Correlation analysis between the APN polymorphism and traditional Chinese medicine syndromes in acute ischemic stroke patients of Xinjiang Uygur and Han nationalities

ZHANG Zhen-zhong1, LIANG Xiao-ying2, LÜ Guang-yao2, YAO Hua3   

  1. 1College of Public Health, Xinjiang Medical University, Urumqi 830054, Xinjiang, China
    2Department of Neurology, the Affiliated Hospital of Xinjiang Medical University of Traditional Chinese Medicine, Urumqi 830001, Xinjiang, China
    3Office of the Party Committee, the First Teaching Hospital of Xinjiang Medical University, Urumqi 830054, Xinjiang, China
  • Online:2014-07-25 Published:2014-07-27
  • Contact: YAO Hua (Email: gzybgzyb@163.com)
  • Supported by:

    This study was supported by National Natural Science Foundation of China (No. 81060289).

摘要:

目的 探讨维吾尔族和汉族缺血中风患者急性期中医证候分布特点及演变规律。方法 采集210 例缺血中风急性期患者(维吾尔族89 例、汉族121 例)中医证候信息,根据缺血中风证候要素诊断量表进行中医证候要素辨证,并观察其证候组合形式和演变规律。采用SNaPshot 技术检测维汉两族患者APN 基因单核苷酸多态性,分析二者中医证候组合形式之基因型,探讨不同位点多态性之间的差异及两族患者脂联素(APN)基因多态性与中医证候间的关联性。结果 (1)维吾尔族缺血中风患者以痰湿证为主体、汉族以气虚证为主体;维吾尔族患者发病急性期之中后期以两证相兼为主,汉族以三证相兼为主。(2)维吾尔族急性缺血中风患者APN 基因多态性位点各基因型与其中医证候间无关联性,而汉族患者APN 基因rs6444175G/A、rs1501296G/A 位点各基因型与中医证候组合相关;维汉两族患者APN 基因各位点等位基因频率与中医证候均无关联性。结论 维汉两族急性缺血中风患者具有不同的中医证候分布,其中医证候组合与APN 基因多态性位点基因型和等位基因频率相关。

关键词: 卒中, 脂联素, 基因, 多态性, 单核苷酸, 证候, 中医学, 维吾尔族

Abstract:

Objective  To analyze the characteristics and variation of traditional Chinese medicine (TCM) syndromes in acute ischemic stroke patients of Xinjiang Uygur and Han nationalities, and to investigate the polymorphism of adiponectin (APN) of loci rs182052, rs6444175, rs1501296 and its correlation with TCM syndromes.  Methods  A total of 210 patients with acute ischemic stroke (89 cases of Uygur nationality and 121 cases of Han nationality) were collected for information of TCM syndromes according to the "Diagnostic Scale for TCM Syndromes of Ischemic Stroke", and the APN single nucleotide polymorphism (SNP) of patients was detected by SNaPshot technology. The TCM syndromes between patients of Uygur and Han nationalities were compared and the correlation between TCM syndromes and APN SNP or allele frequency was analyzed.  Results  1) There were different TCM syndromes during acute ischemic stroke between patients of Uygur and Han nationalities. The TCM syndromes in Uygur patients were based on phlegm, while the syndromes in Han patients were based on Qi deficiency. Most of Uygur patients had 2 accumulated TCM syndromes in the middle and late phase of acute ischemic stroke, which was significantly different from Han patients, who mainly had 3 accumulated TCM syndromes. 2) The genotypes of APN were not correlated with TCM syndromes accumulation in Uygur patients, while the genotypes of APN rs6444175G/A and rs1501296G/A were correlated with TCM syndromes accumulation in Han patients. The allele frequency of all three APN genotypes were not correlated with TCM syndromes accumulation in both Uygur and Han patients.  Conclusions  There are different distributions of TCM syndromes and its accumulation between Uygur and Han patients, and the accumulations of TCM syndromes of acute ischemic stroke in Uygur and Han patients are correlated with different APN genotypes.

Key words: Stroke, Adiponectin, Genes, Polymorphism, single nucleotide, Symptom complex, TCM science, Uygur nationality