摘要： 目的 克隆空肠弯曲菌Lulei 株WaaF 基因，并分析其遗传进化关系。方法 聚合酶链反应扩增空肠弯曲菌Lulei 株WaaF 基因，构建pGEM-T-WaaF 质粒，选择正确克隆质粒测序。自美国国家生物技术信息中心Nucleotide 数据库下载5 株吉兰?巴雷综合征相关菌株WaaF 序列，1 株非吉兰-巴雷综合征相关菌株WaaF 序列，利用DNAstar v7.1 软件建立进化树，分析不同菌株间WaaF 基因遗传关系。结果 完成了空肠弯曲菌Lulei 株WaaF 基因的克隆，发现WaaF 基因在吉兰-巴雷综合征相关菌株间存在聚类现象。结论 空肠弯曲菌Lulei株WaaF 基因是807 bp的片段，与Lichang株遗传进化关系最近。
关键词:
弯曲杆菌,
空肠,
基因,
序列分析,
DNA,
进化,
遗传学
Abstract: Objective To clone the WaaF gene of Campylobacter jejuni, and analyse its relationship with WaaF genetic evolution. Methods Amplified WaaF gene of Campylobacter jejuni Lulei by PCR, and constructed pGEM-T-WaaF cloning plasmid. Downloaded five WaaF associated with Guillain-Barré syndrome (GBS) and one WaaF not associated with GBS, and then constructed phylogenetic tree. Results pGEM-T-WaaF cloning plasmid was constructed successfully. WaaF presented cluster phenomenon in Campylobacter jejuni associated with GBS. Conclusion WaaF gene of Campylobacter jejuni Lulei is the fragment of 807 bp, and has the nearest relationship with the genetic evolution of Lichang.
Key words:
Campylobacter jejuni,
Genes,
Sequence analysis, DNA,
Evolution,
Genetics
邢丛丛，徐飞，白欣立，付金生，李文倩，卢晓卫，马海阳，李震中. 空肠弯曲菌Lulei株WaaF 基因克隆与序列分析[J]. 中国现代神经疾病杂志, 2012, 12(2): 158-160.
XING Cong-cong1, XU Fei1, BAI Xin-li2, FU Jin-sheng1, LI Wen-qian1, LU Xiao-wei1, MA Hai-yang1, LI Zhen-zhong1. Cloning and alignment of WaaF gene of Campylobacter jejuni Lulei[J]. Chinese Journal of Contemporary Neurology and Neurosurgery, 2012, 12(2): 158-160.