摘要： 目的 应用Fluoro-Jade C（FJC）染色方法观察小鼠匹罗卡品癫模型丘脑神经元变性情况，以了解丘脑结构在慢性颞叶癫发生中的病理变化和癫反复发作的神经学基础。方法 以盐酸匹罗卡品腹腔注射（220 mg/kg）制备小鼠癫持续状态模型。脑组织切片经FJC 染色后，荧光显微镜下观察FJC 阳性细胞形态和在丘脑的整体分布情况。结果 匹罗卡品模型组小鼠FJC 阳性细胞呈神经元形态，丘脑结构损害呈连续性，功能不同的丘脑核其细胞损害程度有所不同。结论 采用FJC 染色技术观察匹罗卡品癫持续状态小鼠模型丘脑神经元变性情况，有利于更好地理解颞叶癫的中枢神经系统长期病理变化和自发性反复发作机制。

关键词: 荧光染料, 癫持续状态, 毛果芸香碱, 神经变性, 疾病模型, 动物

Abstract: Objective To detect degenerative neurons by Fluoro-Jade C (FJC) that may occur in the thalamus of pilocarpine-treated mice and to understand the long-term pathologic changes of thalamus and recurrent seizure mechanism in chronic epilepsy. Methods Male adult mice (Kunming stain, n = 10) were divided into pilocarpine-treated group (n = 5) and controls (n = 5). Pilocarpine-treated mice were sacrificed at 12 h after status epilepticus. Brain coronal sections in the thalamus level were subject to FJC staining. Finally, FJC-stained brain sections were examined under fluorescence microscope to identify the shape and overall distribution of the Fluoro-Jade C positive cells in the thalamus. Results The FJC-positive staining cells showed a bright green color in the thalamus tissue and fine processes with neuronal profiles. There was a continuity of thalamic damage and neuronal damage in functionally heterogenous thalamic nuclei. They were not detected in control animals. Conclusion This study successfully applied FJC staining to demonstrate massive neurodegeneration in the thalamus, which will benefit further understanding of long-term brain pathologic changes and recurrent seizure mechanism in temporal lobe epilepsy.

Key words: Fluorescent dyes, Status epilepsy, Pilocarpine, Nerve degeneration, Disease models, animal